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18
Midori Green, Midori Green Advance and Midori Green Direct
Three non carcinogenic alternative dyes for staining of nucleic acids
All Midori Green dyes can be used as a safer alternative to the traditional Ethidium Bromide for detecting nucleic acid fragments in agarose gels.
These newly developed dyes are non carcinogenic and according to the Ames test they cause significantly lower mutation rates than Ethidium Bromide.
Midori Green and Midori Green Advance can be used for in gel staining as well as for post staining, whereas Midori Green Direct will be mixed just with the samples.
Midori Green
The most cost effective non Ethidium Bromide based DNA stain: Just 1,5 -2µl are
enough for staining of 100 ml agarose gel.
Fig. 1: Summary of Ames test results: Mutagenicity ratio of Midori Green DNA Stain and Ethidium Bromide with
metabolic activation (S9) in TA98 mutagenicity assay. According to the results of the Ames test, Midori Green DNA
Stain is significantly less mutagenic compared to Ethidium Bromide..
Ordering information
Midori Green 1 ml MG02
Midori Green Advance
Besides the positive effect for your health this dye shows a very high sensitivity even
for small fragments as well as a superior signal to noise ratio. An unspecific background
because of non specific binding of dye molecules to agarose is avoided. 4-6 µl are
enough for the staining of 100 ml agarose gel.
Ordering information
Midori Green Advance 1 ml MG04
Midori Green Direct
Midori Green Direct stain represent a new and safe class of nucleic acid stains for
visualization of double-stranded DNA, single-stranded DNA and RNA in agarose gels.
In contrast to most other non Ethidium Bromide based dyes this product is just added to
your samples. Midori Green Direct includes a 10x sample loading dye. You do not need
to add any other dyes to both gel matrix and running buffers.
The DNA sample and Midori Green Direct will be mixed in a ratio of 10:1. Because the
agarose gel won‘t be stained, the signal noise ratio is excellent.
Fig. 4: HeLa cells were incubated at 37°C with 1X of SYBR Green I and Midori Green Direct dyes.
Images were taken following incubation for 30 min. SYBR Green I entered into cells rapidly as evident from
the bright green nuclear staining. However Midori Green Direct was unable to cross cell membranes as
shown by the lack of any fluorescence staining.
Ordering information
Midori Green Direct 1 ml MG06
1 2 3 4 5 6 1 2 3 4 5 6
Fig. 2: FastGene ® 100 bp DNA ladder (MWD 100) stained with Midori Green
(left side, lanes 1 & 6) and Ethidium Bromide (right side, lanes 1 & 6).
Ethidium Bromide Midori Green Advance
Phone: ++49/2421/2084690 | Fax: ++49/02421/2084691 | Email: info@nippongenetics.eu
20µl 10µl 20µl 10µl 20µl 10µl 20µl 10µl
1,5kb
1,5kb
Fig 3: Comparison of sensitivity between Ethidium Bromide (left) and Midori
Green Advance (right) using an UV-Transilluminator (invers projection). Data
kindly provided by Ms. Abel, Heinrich-Heine-University Düsseldorf; Germany.
4µl 3µl 2µl 1µl 0.8µl 0.7µl
1:2 1:5 1:10 1:25
-2,8ng
1 2 3 4 5 6
1kb
500bp
100bp
Fig.5: Agarose gel analysis of different volumes of
FastGene ® 1 kb DNA ladder. Even 2 µl of the DNA
ladder can be detected easily, although 5 µl (0.5 µg) is
recommended.