Inducción de muestras de esputo para el estudio citológico y de ...

Núm. 5 Inducción de muestras de esputo para el estudio citológico (III) 265
17. Hamid Q, Azzawi M, Ying S, Moqbel R, Wardlaw
AJ, Corrigan CJ, et al. Expression of mRNA for
interleukin-5 in mucosal biopsies from asthma. J
Clin Invest 1991; 87: 1541-6.
18. Lai CKW, Ho ASS, Chan CHS, Tang J, Leung
JCK, Lai KN. Interleukin-5 messenger RNA
expression in peripheral blood CD4+ cells in asthma.
J Allergy Clin Immunol 1996; 97: 1320-8.
19. Gelder CM, Thomas PS, Yates DH, Adcock MI,
Morrison JFJ, Barnes PJ. Cytokine expression in
normal, atopic, and asthmatic subjects using the
S. Quirce Gancedo
Servicio de Alergia
Fundación Jiménez Díaz
Avda. Reyes Católicos, 2
28040 Madrid
37
combination of sputum induction and the polymerase
chain reaction. Thorax 1995; 50: 1033-7.
20. Chomczynski P, Sacchi N. Single step method of
RNA isolation by acid guanidinium thyociantephenol-chloroform
extraction. Anal Biochem 1987;
162: 156-9.
21. Pizzichini MMM, Pizzichini E, Clelland L, Efthimiadis
A, Mahony J, Dolovich J, Hargreave FE.
Sputum in severe exacerbations of asthma. Kinetics
of inflammatory indices after prednisone treatment.
Am J Respir Crit Care Med 1997; 155: 1501-8.