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Effet de la sonication sur les rendements d'extraction ... - Sciencelib

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Résumé<br />

Quatre algues marines Cystoseira fimbriata et Cystoseira mediterranea, <strong>de</strong>ux phéophyceés et <strong>de</strong>ux<br />

chlorophycées : Enteromoprpha intestinalis et Ulva <strong>la</strong>ctuca sont soumises à <strong>de</strong>ux métho<strong>de</strong>s<br />

d’extractions <strong>de</strong> leurs pigments, l’extraction soli<strong>de</strong>-liqui<strong>de</strong> (SLE) utilisant l’acétone ou le méthanol<br />

comme solvant et l’extraction SLE assistée par <strong>de</strong>s ultrasons (UAE).<br />

La <strong>sonication</strong> améliore nettement <strong>les</strong> taux d’extraction <strong>de</strong>s pigments, notamment celui <strong>de</strong>s<br />

caroténoï<strong>de</strong>s qui augmente en moyenne <strong>de</strong> 57.06 % chez l’espèce C.fimbriata.<br />

L’optimisation <strong>de</strong> l’extraction <strong>de</strong>s chlorophyl<strong>les</strong> après passage <strong>de</strong>s extraits aux ultrasons a permis<br />

d’augmenter <strong>les</strong> taux d’extraction <strong>de</strong>s chlorophyl<strong>les</strong> a, <strong>de</strong> 19.09% (C.mediterranea) à 55.12 %<br />

(C.fimbriata) dans <strong>les</strong> extraits d’acétone, et <strong>de</strong> 21.88% (C.fimbriata) à 64.43 % (U.<strong>la</strong>ctuca) dans<br />

<strong>les</strong> extraits du méthanol.<br />

Les teneurs <strong>de</strong>s chlorophyl<strong>les</strong> b, ont également connu <strong>de</strong>s augmentations sous l’effet <strong>de</strong>s ultrasons,<br />

el<strong>les</strong> sont augmentées <strong>de</strong> 29.51% (E.intestinalis) à 67.41% (U.<strong>la</strong>ctuca) dans <strong>les</strong> extraits d’acétone,<br />

et, <strong>de</strong> 5.13% (U.<strong>la</strong>ctuca) à 54% (E.intestinalis) dans l’extraction au méthanol.<br />

Mots clés : Algues marines ; Béjaïa ; UAE ; SLE ; caroténoï<strong>de</strong>s ; chlorophyl<strong>les</strong>.<br />

Abstract<br />

In this study, pigments of four species of marine algae, Cystoseira fimbriata et Cystoseira<br />

mediterranea, two Pheophyceae) and two Chlorophyceae: Enteromoprpha intestinalis et Ulva<br />

<strong>la</strong>ctuca are extracted using two methods : SLE or Solid-liquid extraction with methanol or acetone<br />

solvent and ultrasound-assisted extraction or UAE .<br />

The <strong>sonication</strong> improves sharply the rates of extraction of pigments, notably that of the carotenoïds<br />

which increases on average by 57.06 % in C.fimbriata.<br />

The optimization of the extraction of chlorophylls after passage of extracts in the ultrasounds<br />

allowed to increase the rates of extraction of chlorophylls a, of 19.09 % ( C.mediterranea) in 55.12<br />

% ( C.fimbriata) in the extracts of acetone, and 21.88 % (C.fimbriata) in 64.43 % (U.<strong>la</strong>ctuca) in the<br />

extracts of the methanol.<br />

The contents of chlorophylls b, also knew increases un<strong>de</strong>r the influence of the ultrasounds, they are<br />

increased by 29.51 % ( E.intestinalis) in 67.41 % ( U.<strong>la</strong>ctuca) in the extracts of acetone, and, by<br />

5.13 % ( U.<strong>la</strong>ctuca) in 54 % ( E.intestinalis) in the extraction in the methanol.<br />

Keywords : Seaweeds ; Béjaïa ; UEA ; SLE ; carotenoids ; chlorophylls.<br />

ScienceLib Editions Mersenne : Volume 5 , N ° 130106<br />

ISSN 2111-4706<br />

SADI Hamza<br />

2

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