Original article - Blood Transfusion

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HPA genotyping in southern ItalyTable VI - Comparison of the frequency of HPA antigens in Caucasian populations and other populationsHPA antigens Studied subjects Dutch USA Finn Japanese(Italians) population 11 white population 12 population 13 population 14,151a 95.5% 97.9% 98% 99% 100%1b 28% 28.8% 20% 26.5% 0.3%2a 97% 100% 97% 99% 99.2%2b 26.5% 13.2% 15% 16.5% 19.7%3a 85.5% 81% 88% 83.5% 85.1%3b 54% 69.8% 54% 66.5% 66.2%4a 100% 100% 100% 100% -4b 0.5% 1% 1% 1% -5a 99.5% 100% 98% 99.5% 99.0%5b 15.5% 19.7% 21% 10% 7.0%6a 100% - - 100% 99.7%6b 0.5% - - < 1% 5.13%visualised horizontally using a molecular weight marker toenable evaluation of the results. The detection of DNAfragments was carried under UV light.The PCR-SSP analysis was performed according to theworking protocol of Domino Protrans HPA System (NuclearLaser, Settala, MI, Italy).Out of 200 genomic DNA donor samples tested, 50 wereexamined again with a direct sequencing as well as PCR–SSP analysis, to validate our results.ResultsGenotypic and antigenic frequencies of HPA-1, -2, -3, -4, -5, -6 in the analysed samples from blood donors aresummarised in table V. Our results from 12 couples of parentsof neonates born with a severe form of FNAITP showedincompatibility only for HPA-1 system. Theseincompatibilities were responsible for the FNAITP andconfirmed by the presence of offending antibodies inmaternal serum (10 anti-HPA-1a, 2 anti-HPA-1b).Figure 2 shows a diagrammatic example of PCR-SSPanalysis of the HPA 1-6 systems.DiscussionEach HPA system consists of two allelic variants, nowknown as “a”, high frequency, and “b”, low frequency.These allelic variants are caused by single point mutations,which result in single amino acid changes between the twodifferent gene products.In our study the frequencies of the alleles in most ofthe HPA systems are comparable to those described byother authors for the European Caucasian population 11 . Inconosciute, rispettivamente, come "a", ad alta frequenza, e"b", a bassa frequenza. Le varianti sono causate da unasola mutazione puntiforme che determina un cambiamentoin un singolo amminoacido in due diversi prodotti genici.Le frequenze per i più importanti sistemi HPA, ottenute nelnostro studio, sono paragonabili a quelle trovate da altriAutori nei caucasici europei 11 . Al contrario, la frequenzadell'antigene HPA-1b è più alta nel nostro campione rispettoa quelle della popolazione bianca statunitense e dellapopolazione giapponese (Tabella VI) 11-15 .Tali dati avvalorano ulteriormente che la frequenza deglialleli HPA-4b e HAP-6b è, nei caucasici, molto bassa (
G Fratellanza et al.contrast, the frequency of the HPA-1b antigen in oursamples was higher than that in Japanese and white USApopulations (Table VI) 11-15 .Our data further corroborate that the frequency of theless common HPA-4b and HPA-6b alleles is extremely low(
Page 3 and 4: G Fratellanza et al.Table I - Seque
Page 5: G Fratellanza et al.Table V - HPA g
Page 9: G Fratellanza et al.References1) Kr

Original article - Blood Transfusion

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