Universiteit van die Oranje-Vrystaat - SAMJ Archive Browser

%I VEBGELYKING TUSSEN DIE MAAGDEURGANG VAN
VEBSKKLEMlE SUBSPANSE
SAW VOL (W 7 DEC I991 6Ol
RESUCTAA'E
Die gemiddelde l (f 1 standaardafwyking)m: am-
54 minute (-c 24 mnrute lemoemap - l uur 33 minute (k 26
minute), aqelpg - l usm 45 &ute (f 32 minute), oolk - 2 urn 39
minute (a 2 mulute) en die ligte ontbyt - 3 uur en 6 minute (+ 42
minute).
Daar was geen beduideneje vesldl ~ t n
appelen
lemmmp nie, roaarroaar daat tussen die
kQ&stp van ai die ander stowwe (p < 0,05).
PRODUCTION OFA MONOCLONAL ANTIBODY
AGAINST Tome SHOCK -ROME TOXIN-1
(TSST-1)
~~~truwi~l L El3 Dowdle2 PL Bothal
'Department of Medical Microbiology, UOFS
2~epartment of Qinical Science and Immunology, UCT
Toxic shock syndrome ('ES) is a severe illness affecting
mainly young menstruating females. It presents with a
sudden onset of diverse symptoms and signs, including shock
and multisystem involvement. The major causative agent of
the disease is toxic shock syndrome toxin-l (TSST-l)
produced by 30% of all clinical isolates of Stuphylococcus
aureus.
P
The aim of this study was to roduce a monodonal antibody
against TSST-1 and to app y the antibody in an enzyme
linked immunosorbent assay (=SA) developed' for
detection d the toxin.
TSST-1 was purified from culture supematant by ethanol
precipitation and ion exchange chromtoj$raphy. Balb/c
mice were immmised with the toxin. AnQbody producing
hybridomas were developed by fusing mouse spleen
lymphocytes and Sp2/0 myelorna cells with addition of
polyethylene glycol (PEG) to the cell suspe11sion. A
standard EUSA technique was developed for screening of
hybridoma cultures for anti-TSST-l antibodies, and also for
detection of TSST-1 production by clinical isolates of
Swells
A total of 1% clones developed from two fusion procedures.
Ten of these clones were anti-TSST-l producers. One
positive clone was selected for subclonin by limiting
d
dilution, resulting in five anti-TSST-l pr ucing clones.
Forty persent of S. mrem clinical isolates examined in this
study produced TSST-1.
Although the diagnosis of.TSS is made mainly on the
clinical evaluation of the patient, the use of a monoclonal
antibody against the toxin my have diagnostic v ah~