Utveckling av metodik för mätning av pO2 i vävnad Development of ...

Innehållsförteckning
1 INLEDNING........................................................................................................................ 10
1.1 PROBLEM ........................................................................................................................ 10
1.2 UPPDRAG ........................................................................................................................ 10
1.3 UPPDELNING.................................................................................................................... 10
2 FYSIOLOGI ........................................................................................................................ 12
2.1 GASUTBYTE I KROPPEN.................................................................................................... 12
2.1.3 Partialtryck.............................................................................................................. 12
2.1.1 Lungorna ................................................................................................................. 13
2.1.2 Inre gasutbyte.......................................................................................................... 14
2.2 SYRETRANSPORTEN I BLODET.......................................................................................... 14
3 ELEKTROKEMISKA PROCESSER............................................................................... 15
3.1 ELEKTRODREAKTIONER................................................................................................... 15
3.2 DUBBELLAGERSKAPACITANS .......................................................................................... 16
4 SYRGASMÄTNING........................................................................................................... 17
4.1 BIOSENSORER.................................................................................................................. 17
4.1.1 Elektrokemiska sensorer ......................................................................................... 17
4.2 POLAROGRAFI ................................................................................................................. 17
4.2.1 Clark-elektroden...................................................................................................... 19
4.2.2 Pulsad polarografi .................................................................................................. 29
5 ANVÄNDNINGSOMRÅDEN............................................................................................ 20
5.1 HYPERBAR OXYGENTERAPI, HBO ................................................................................... 20
5.2 CANCERFORSKNING......................................................................................................... 20
5.3 HJÄRNISCEMI................................................................................................................... 21
5.4 SÅRLÄKNINGSPROCESSES................................................................................................ 21
6 MATERIAL OCH METODER ......................................................................................... 22
6.1 ELEKTRODSYSTEM .......................................................................................................... 22
6.1.1 Biokompatibla material för elektroder.................................................................... 22
6.1.2 Temperaturberoende ............................................................................................... 23
6.1.3 Syreförbrukning....................................................................................................... 23
6.2 UTVECKLING AV ARBETSELEKTRODEN............................................................................ 23
6.2.1 Guld......................................................................................................................... 23
6.2.2 Isolering .................................................................................................................. 24
6.2.3 Design...................................................................................................................... 26
6.3 UTVECKLING AV REFERENSELEKTRODEN ........................................................................ 28
6.3.1 Gelatin..................................................................................................................... 28
6.4 PO 2-MONITOR.................................................................................................................. 30
7.1 IN VITRO .......................................................................................................................... 33
7.1.1 Utrustning................................................................................................................ 33
7.2 IN VIVO............................................................................................................................ 37
7.2.1 Utrustning................................................................................................................ 37
7.2.2 Utförande och resultat ............................................................................................ 37
8 DISKUSSION OCH SLUTSATSER ................................................................................. 38
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