Program ve Bildiri Özetleri Kitabı - Ankara Mikrobiyoloji Derneği

KNF3
SEPTIFAST - A MULTIPLEX REAL TIME PCR METHOD FOR THE DETECTION AND
IDENTIFICATION OF BACTERIAL AND FUNGAL PATHOGENS IN THE BLOODSTREAM
Roche Diagnostics katkılarıyla
Gorm LISBY, MD, PhD
Department of Clinical Microbiology 445, Hvidovre Hospital, Kettegård Alle 30, DK-2650
Hvidovre, Denmark.
KONFERANSLAR
Worldwide, severe sepsis is increasingly a cause of death, and rapid, correct initial antimicrobial
treatment reduces mortality. Although blood culture is considered to be the gold standard, the etiological
agent(s) cannot always be found by this technology. A novel multiplex PCR test (SeptiFast),
that can be performed in less than six hours and can identify a panel of 20 bacterial and fungal species
directly from blood, was compared with blood culture in a multicenter trial in patients with
suspected bacterial or fungal sepsis. Five hundred and fi ft y-eight paired samples of blood culture
and SeptiFast from 359 patients were evaluated. Blood culture found 96 isolates and SeptiFast identifi
ed 186 microorgansims. Of the 96 blood culture isolates, 50 were also detected by SeptiFast and 22
blood culture isolates were identifi ed as contaminant isolates. Eighteen of the remaining 24 blood
culture clinical isolates were not detected by SeptiFast and six blood culture isolates were not included
in the SeptiFast masterlist. SeptiFast detected 186 microorganisms, of which 12 were considered
to be contaminants. Of the 174 clinical microorganisms identifi ed by SeptiFast, 50 were also detected
by blood culture. 67 of the remaining 124 SeptiFast positive and blood culture negative microorganisms
were also identifi ed in routine cultures from clinical relevant anatomical sites from the patient.
Future clinical utility studies will reveal if SeptiFast can provide a rapid, signifi cant add-on advantage
to blood culture in the detection of blood stream pathogens.
11