Pełny tekst (PDF) - Progress in Plant Protection/Postępy w Ochronie ...

Nicienie i ich identyfikacja 1775
STEFAN WOLNY, GRAŻYNA WINISZEWSKA,
EWA DMOWSKA, TADEUSZ MALEWSKI
NEMATODES CAUSING LOSS OF PRODUCTION PLANT IN POLAND
AND METHODS OF THEIR RAPID IDENTIFICATION
SUMMARY
In Poland, it has been found so far about 260 species of plant and fungi parasitic nematodes.
Among the nematodes feeding on plants there are species that have the status of quarantine pests.
Before taking the decision to control nematodes, it is necessary to identify the pest species. The
identification of nematodes is difficult because of the paucity of easily scorable diagnostic morphological
characters. Consequently, molecular identification tools could solve this problem. For
molecular identification of species are suitable different PCR-based methods, DNA barcoding and
DNA microarrays. The mitochondrial cytochrome oxidase c subunit 1 (COI) gene is one of the
most popular markers for species identification across the animal kingdom. Species identification
based on COI sequence received name DNA barcoding. DNA microarrays, first created 20 years
ago, are well established and able to differentiate hundreds of specimens simultaneously. Microarray
technologies, which involve hybridization of short specific probes to target DNA and
subsequent detection of the hybridization signal, have been widely used as diagnostic tools.
Key words: plant parasitic nematodes, taxonomy, quarantine, rapid identification, DNA