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8. Sažetak i naslov na engleskom jezikuROLE OF NITRIC OXIDE ON BLOOD-BRAINPERMEABILITY IN COMMON CARP (Cyprinus carpio L.)The aim of this study was to determine the effect of nitric oxide (NO) on blood-brain barrierpermeability in vivo in common carp by using glyceryltrinitrate (GTN) as an NO donor. Wehave established the reversibility and duration of increased permeability of the blood-brainbarrier; we have observed, described and determined the duration of brain edema formation,and defined the similarities of the mechanism of NO regulated increase of the blood-brainbarrier permeability in lower vertebrates and the species investigated so far.We experimentally treated two-year-old carp (Cyprinus carpio L.) with 1 mg glyceryltrinitrate/kgbody weight. Total nitrate and nitrite concentrations in the carp sera were determined15 minutes, 1 hour, 3 hours, 6 hours, 8 hours, 12 hours, and 24 hours after treatment by usingthe commercial kit. In both control and treated groups blood-brain barrier permeabilitywas analyzed at 6, 12, and 24 hours after GTN application based on Evans blue leakage inthe brain specimens; the specimens were also observed under a fluorescent microscope.Brain edema was determined by using the wet/dry method and described on hemalaun-eosinstained preparations under a light microscope 6 and 24 hours after GTN treatment.The total concentration of nitrite/nitrate showed a biphasic pattern with maximum concentrations15 minutes after GTN application, mean values ± SEM: 188.1 ± 23.82 μmol/l, and after6 hours (236.4 ± 64.67 μmol/l) and 8 hours (255.2 ± 25.81 μmol/l). The dynamic changes ofnitrate concentrations followed that of total nitrite/nitrate concentrations. Maximum valueswere observed 15 minutes after GTN application (180.2 ± 25.76 μmol/l), and after 6 (232.4 ±58