Test af membran bioreaktor-teknologier - Naturstyrelsen
Test af membran bioreaktor-teknologier - Naturstyrelsen
Test af membran bioreaktor-teknologier - Naturstyrelsen
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<strong>Test</strong> <strong>af</strong> Membran<strong>bioreaktor</strong>-<strong>teknologier</strong> – Bilag 4<br />
Bilag 4:<br />
Fouling <strong>af</strong> <strong>bioreaktor</strong><strong>membran</strong>er-<br />
Introduktion til foulingfænomenet og metodeoversigt til karakterisering<br />
<strong>af</strong> founlinglaget<br />
Udarbejdet <strong>af</strong> TI<br />
INDHOLDSFORTEGNELSE<br />
1 INTRODUKTION .............................................................................................................2<br />
1.1 Introduktion til foulingfænomenet og teorien herom ........................................................2<br />
1.2 Introduktion til metoder til karakterisering <strong>af</strong> foulinglaget ................................................4<br />
2 KEMISKE/FYSISKE METODER......................................................................................4<br />
2.1 Prøveforberedelse ...........................................................................................................4<br />
2.2 Ekstraktion <strong>af</strong> ekstracellulær polymerisk substans (EPS-ekstraktion).............................5<br />
2.3 Kulhydratbestemmelse ....................................................................................................5<br />
2.4 Protein- og humusforbindelsebestemmelse ....................................................................6<br />
2.5 Blandet mikstur suspenderet tørstof (MLSS)...................................................................6<br />
2.6 Størrelseseksklusionkromatogr<strong>af</strong>i (SEC) (HPLC-SEC) ...................................................6<br />
2.7 Bestemmelse <strong>af</strong> kemisk oxygenforbrug (COD) ...............................................................7<br />
2.8 Bestemmelse <strong>af</strong> total organisk kulstof (TOC) ..................................................................8<br />
2.9 Opløst organisk kulstof (DOC).........................................................................................8<br />
2.10 Total fosfor-, nitrat- og ammoniakbestemmelse ..............................................................8<br />
2.11 (Miljø) scanning elektron microscopi (ESEM/SEM).........................................................8<br />
2.12 Farvning <strong>af</strong> organisk foulinglag og konfokal laserskanningmikroskopi............................9<br />
2.13 3D fluorescens excitations/emissions-matrix (3D fluorescence EEM) spelroskopi .......10<br />
2.14 Energi dispersiv røngten (EDX) spektroskopi................................................................11<br />
2.15 Induktivt koblet plasma-massespektroskopi (ICP-MS) ..................................................12<br />
3 MIKROBIOLOGISKE METODER..................................................................................12<br />
3.1 PCR ...............................................................................................................................12<br />
3.2 Kloning og 16S rDNA sekventering ...............................................................................12<br />
3.3 FISH (Fluorescens In Situ Hybridisering) ......................................................................13<br />
3.4 DGGE (Denaturing Gradient Gel Electrophoresis)........................................................13<br />
3.5 T-RFLP (Terminal Restriction Fragment Length Polymorphism) ..................................13<br />
4 UDSTYRS- OG METODEOVERSIGT...........................................................................14<br />
5 LITTERATURLISTE.......................................................................................................15<br />
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