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MERG - Universitetet i Oslo

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<strong>MERG</strong><br />

A molecular phylogeny of the brown-spored agarics<br />

(Basidiomycota)<br />

Objectives of the project<br />

• Re-analyse the current classifications schemes of the brown-spored agarics and define<br />

monophyletic groups.<br />

• Track and analyse the evolution of various mycorrhizal and saprotrophic life-forms.<br />

• Analyse infrageneric molecular phylogeny in Galerina, and test species boundaries and character<br />

evolution, using a multi-locus phylogenetic approach.<br />

• Analyse infrageneric molecular phylogeny in Cortinarius, and track the evolution of chemical,<br />

morphological and ecological characters in light of the molecular results.<br />

• Perform a critical examination of the G. atkinsoniana and G. marginata species complexes, using a<br />

combination of multi-locus sequencing and AFLP fingerprints.<br />

Galerina marginata, Photo: Klaus Høiland<br />

Project summary<br />

The brown-spored agarics include saprotrophic and mycorrhizal fungi belonging to the families<br />

Bolbitiaceae, Cortinariaceae, Crepidotaceae, and Strophariaceae. The taxonomy is largely based on<br />

morphological and ecological characters, as in most groups of fungi. In this PhD project, our main goal<br />

is to support species and genus level distinction by using DNA sequence data to infer phylogenetic<br />

relatedness and character evolution, and subsequently, to approach a phylogenetic classification of the<br />

brown-spored agarics. Multilocus sequence data sets will be analyzed using parsimony and maximum<br />

likelihood based phylogenetic methods. A special emphasis is put on the genus Galerina, which<br />

includes saprotrophic and bryophilous species producing small, mycenoid and yellow to brownish<br />

basidiocarps.<br />

The team<br />

Øyvind Stensrud (PhD), Trond Schumacher, Håvard Kauserud, Klaus Høiland + external collaborators.

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