MERG - Universitetet i Oslo
MERG - Universitetet i Oslo
MERG - Universitetet i Oslo
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<strong>MERG</strong><br />
Regulatory RNA and the origin of animal multicellularity<br />
This is a project in the field of evolutionary developmental (evo-devo) biology. The overall aim is to<br />
greatly improve our understanding of the transition from unicellular eukaryotes to multicellular animals.<br />
This represents a mega-leap in the evolution of eukaryote genome and cell organisation that hence of<br />
fundamental importance in biology and medicine.<br />
Project summary<br />
We will in this project focus on the unicellular relatives of animals termed Choanozoa. On the basis of<br />
current status in the field we aim toward bringing the field of evo-devo a step further by rigorous<br />
examination of the role of non-coding and regulatory RNA in the evolution of animal multicellularity. This<br />
will be achieved by studying selected Choanozoa species and using bioinformatics and highthroughput<br />
sequencing approaches.<br />
Objectives of the project<br />
In this project we will investigate:<br />
• RNAi machinery losses and gains in the genomes of single-celled ancestors of animals<br />
• The miRNA pathway evolution in animals compared to other eukaryotic supergroups<br />
• Whether the miRNA and piRNA pathways have been essential for development of multicellularity<br />
• Regulatory RNAs in single celled Choanoza that are not derived from RNAi precursors<br />
• Evolution and function of the RNAi protein machinery<br />
• Whether genetic processes, such as gene duplications, lineage-specific gene loss or horizontal gene<br />
transfer took part in shaping the regulatory RNA machinery in Choanozoa<br />
The team<br />
Jon Bråte (Post Doc), Ralf Neumann (PhD), Tom Kristensen, Paul Grini, Dag Klaveness, Kamran<br />
Shalchian-Tabrizi, Maja Adamska (SARS, Bergen), Inaki Ruiz (Univ Barcelona, Spain), Yves Van de<br />
Peer (Univ. of Gent, Belgium)<br />
23<br />
Sphaeroeca, a colony of<br />
choanoflagellates (aproximately 230<br />
individuals). Photo: Dhzanette.<br />
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