SATS 2009 Final Program - Scandinavian Association for Thoracic ...

S10
SATS BASIC SCIENCE ORAL ABSTRACT SESSION
S10:1
INJECTION OF MESENCHYMAL STEM CELLS MODIFIED WITH VEGF GENE IN ISCHEMIC
MYOCARDIUM IMPROVES CARDIAC FUNCTION IN RATS
Hua Ping 1 , Yang Yanqi 2 , Chen Ju 1 , Peng Jiangzhou 1 , Chen Bosheng 1 , Han Jie 1 , Wang Youyu 1
1) The 2nd hospital Sun Yat-Sen University, China, 2) University Hospital, Linköping, Sweden
Objective
To observe effect of implantation of mesenchymal stem cells (MSCs) transfected by vascular endothelial growth
factor (VEGF) on myocardium regeneration and angiogenesis in ischemic region, and consequent cardiac function in
rats with acute myocardial infarction (AMI).
Methods
MSCs of Sprague Dawley rat were isolated, cultured, and labeled then with bromodeoxyuridine (BrdU). Some
of them were transfected by adenovirus vector encoding VEGF (Ad.VEGF) gene. AMI was created by ligation of
LAD artery in 40 rats. Four weeks after the ligation, left ventricle ejections fraction (LVEF) was measured with
echocardiography. The rats were divided into four groups with 10 rats in each. Group I: implantation of MSCs
transfected by Ad.VEGF; Group II: implantation of MSCs; Group III: injection of Ad.VEGF; and Group IV: untreated.
Four weeks thereafter, myocardium regeneration and angiogenesis were evaluated with immunohistochemistry.
LVEF measurement was repeated.
Results
MSCs labeled with BrdU were found in the ischemic region in group I and II with incorporation into capillaries, and
stained by TnT antibody. The capillary density of 14.4±1.3/high power field (HPF) in group I and 13.6±1.1/HPF
in group III were significantly higher than that of 5.9±1.7/HPF in group II and 0.00/HPF in group IV (P