SATS 2009 Final Program - Scandinavian Association for Thoracic ...

P01:32
RECIPIENT CELLS IN BRONCHIAL ALLOGRAFTS
Vainikka Tiina 1 , Päiväniemi Outi 2 , Musilová Petra 3 , Alho Hanni 1 , Maasilta Paula 1 ,
Aittomäki Kristiina 1 , Salminen Ulla-Stina 1
1) Helsinki University Hospital, 2) Tampere University Hospital, Finland 3) Veterinary Research Institute, Czech Republic
Lung transplantation is accepted therapy for end-stage pulmonary diseases. The main limitation on long-term
survival is obliterative bronchiolits (OB). It is considered a manifestation of chronic allograft rejection. Histologically
OB is manifested as epithelial cell injury, inflammation, fibrosis, and obliteration of the small airways. We studied
recipient cells in bronchial allografts.
29 random-bred pigs were used. Adequate, inadequate or no immunosuppression was given. 2 received control
autografts and 9 male recipients received bronchial allografts from female donors. A series of allografts were
transplanted subcutaneously on the ventral side of donors and were harvested serially during the follow-up.
Histology (H&E) and y-chromosomes (FISH-method) using pig-spesific DNA-label were assessed. Additional 5
female recipients received bronchial allografts from male donors. Samples of lung, liver, kidney and spleen were
taken after 3 months to study y-chromosomes in female recipient organs (FISH).
In male recipients with none or inadequate immunosuppression, rapid epithelial destruction occurred in bronchial
allografts preceding obliteration. Adequate immunosuppression resulted in graft patency until 3 months (p