TRIzol Reagent - Invitrogen
TRIzol Reagent - Invitrogen
TRIzol Reagent - Invitrogen
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A260/A280 Ratio < 1.70 for DNA<br />
Large pellet after initial ethanol precipitation<br />
Aqueous layer appeared after addition of 100% ethanol<br />
Protein Isolation using <strong>TRIzol</strong><br />
Detailed Protocol Steps for Protein Isolation with <strong>TRIzol</strong><br />
Protein Precipitation<br />
Protein Wash<br />
Redissolving the Protein Pellet<br />
Additional Notes for Protein Isolation using <strong>TRIzol</strong><br />
Recovering phosphorylated proteins<br />
Membrane proteins<br />
Analysis of proteins before precipitation<br />
Interference of SDS with protein quantitation<br />
Analyzing proteins by RIA<br />
Precipitation of SDS during dialysis<br />
<strong>TRIzol</strong> LS<br />
Applications and Yields with <strong>TRIzol</strong> LS<br />
RNA Isolation with <strong>TRIzol</strong> LS<br />
Detailed Protocol Steps for RNA Isolation with <strong>TRIzol</strong> LS<br />
Homogenization<br />
Phase Separation<br />
RNA Wash<br />
RNA Precipitation<br />
Redissolving the RNA Pellet<br />
Additional Notes for RNA Isolation with <strong>TRIzol</strong> LS<br />
RNAses<br />
Tubes to use with <strong>TRIzol</strong> LS<br />
Troubleshooting of RNA Isolation with <strong>TRIzol</strong> LS<br />
Low Yield of RNA<br />
A260/280 ratio is
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