TRIzol Reagent - Invitrogen

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Vacuum dry the protein pellet for 5-10 min. Dissolve in 1% SDS by pipetting (may need to heat at 50oC). Sediment insoluble
material by centrifugation at 10000 x g for 10 min. at 4ºC. Transfer the supernatant to a clean tube.
For other recommendations for solubilizing the protein pellet, please see Step 3 of Protein Isolation with TRIzol
above.
Additional Notes for Protein Isolation with TRIzol LS
Interference of SDS with protein quantitation
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(back to Protein Isolation with TRIzol LS)
The SDS in the solubilization buffer can interfere with Bradford and Lowry Assay.Methods that do not have detergent-interface
problems, and that do not rely on A260/A280 measurements may be used (traces of phenol may cause overestimation of protein
concentrations).
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REFERENCES
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TRIzol Reagent Key References:
Focus 15.4, 99; TRIzol: A New Reagent for Optimal Single Step Isolation of RNA.
Focus 17:3, 98; Isolation and Long-Term Storage of Proteins From Tissues and Cells Using TRIZOL Reagent.
Focus 20:2, 36 RNA Isolation with TRIzol Reagent (Helpful Tips and Stopping Points)
Focus 20:2, 37; Measuring Absorbance of RNA Samples
Focus 20:3, 80; Modified TRIzol Reagent Protocol for Large mRNA
Focus 20:3, 82; Isolation and Long Term Storage of RNA from Ribonuclease rich Pancreas Tissue.
Focus 21:2, 38; Isolation of Total RNA from Small Samples
Focus 21:3, 66; One-Step RT-PCR to Detect Cytokine/Chemokine Induction in Macrophages
Focus 21:1, 3; One-Step Analysis and Quantification of RNA by RT-PCR: Use of High Temp RT
Focus 21:1, 8; Improved SUPERSCRIPT One-Step RT-PCR Systems
Focus 22:1, 13 Answers to Frequently Asked Questions about RT-PCR
Chomczynski, et. al. (1987) Single Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform
Extraction. Anal. Bioch. 162, 156.
Subhasis Banerjee, Alan Smallwood, Anne E. Chambers and Kypros Nicolaides, 2003. Quantitative recovery of immunoreactive
proteins from clinical samples following isolation of RNA and DNA. BioTechniques 35(3) 450-456).
TRIzol LS Reagent Key References:
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