TRIzol Reagent - Invitrogen
TRIzol Reagent - Invitrogen
TRIzol Reagent - Invitrogen
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Kidney 1 mg 3-4 ug<br />
Skeletal muscles and brain 1 mg 1–1.5ug<br />
Placenta 1 mg 1–4 ug<br />
Epithelial cells 1 x 10 6 cells 8–15 ug<br />
Fibroblasts 1 x 10 6 cells 5-7 ug<br />
*Human Blood was from a single individual. RNA was isolated within 30 min. of being drawn from individual.<br />
**Human Leukocytes were commercially prepared and pooled from 50 individuals.<br />
TYPICAL DNA YIELDS:<br />
Starting Material Amount of Sample DNA Yield<br />
Human Leukocytes 7.0x10 8 cells 1.3 mg<br />
Cultured human, rat, and mouse cells 1 x 10 6 5-7 ug<br />
Fibroblasts 1 x 10 6 5-7 ug<br />
Liver and Kidney 1 mg 3-4 ug<br />
Skeletal muscles, brain, and placenta 1 mg 2-3 ug<br />
*Human Leukocytes were commercially prepared and pooled from 50 individuals.<br />
RNA Isolation with <strong>TRIzol</strong> LS<br />
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Quick overview:<br />
Detailed Protocol Steps for RNA Isolation with <strong>TRIzol</strong> LS<br />
Homogenization<br />
Phase Separation<br />
RNA Wash<br />
RNA Precipitation<br />
Redissolving the RNA Pellet<br />
Additional Notes for RNA Isolation with <strong>TRIzol</strong> LS<br />
RNAses<br />
Tubes to use with <strong>TRIzol</strong> LS<br />
Troubleshooting of RNA Isolation with <strong>TRIzol</strong> LS<br />
Low Yield of RNA<br />
A260/280 ratio is
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