TRIzol Reagent - Invitrogen
TRIzol Reagent - Invitrogen
TRIzol Reagent - Invitrogen
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Final pH 0.1M HEPES (µL) Final pH 1 M HEPES (µL)<br />
8.4 86 7.2 23<br />
8.2 93 7.0 32<br />
8.0 101<br />
7.8 117<br />
7.5 159<br />
Additional Notes for DNA Isolation with <strong>TRIzol</strong> LS<br />
Alternative protocol for DNA isolation<br />
(back to Table of Contents)<br />
(back to Protocol and Application Notes)<br />
(back to DNA Isolation with <strong>TRIzol</strong> LS)<br />
For an alternative protocol for DNA isolation, see the <strong>TRIzol</strong> Additional Notes for DNA Isolation section above under Alternative<br />
procedure for DNA isolation with <strong>TRIzol</strong> <strong>Reagent</strong>.<br />
Troubleshooting DNA Isolation with <strong>TRIzol</strong> LS<br />
For additional information see Troubleshooting DNA Isolation with <strong>TRIzol</strong> Notes Section Above.<br />
Low Yield of DNA<br />
(back to Table of Contents)<br />
(back to Protocol and Application Notes)<br />
(back to DNA Isolation with <strong>TRIzol</strong> LS)<br />
Incomplete homogenization or lysis of samples.<br />
Final DNA pellet incompletely redissolved.<br />
Increase precipitation centrifugation to 5000 x g. May increase yield but may also make pellet more difficult to<br />
solubilize. Confirm centrifugation at 2000-5000 x g, not rpm.<br />
OD reading may vary due to solution the sample is stored in and what it was diluted in prior to quantitation. Wilfinger, et.<br />
al., in (1997) BioTechniques 22.3, 478 report that increases in ionic strength may significantly reduce nucleic acid<br />
absorbance, thereby affecting quantitative determinations based on A260.<br />
A260/280 ratio is
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