FACSAria SORP Standard Operation Protocol Basic Operation

LKS Faculty of Medicine Version 1.1
University of Hong Kong
# If required to change the collecting tubes, click Waste Drawer prior to the
retrieval of the device. Users are reminded to return the waste drawer to
‘SORT’ position following the replacement.
BD FACSAria SORP Operation Manual
During the retrieval of the device
‘SORT’ position following the collecting tube replacement
70.Upon completion, click Sort on the Sort Layout Window and click OK
on the Confirm Prompt; then click ‘Unload’ on Acquisition Dashboard to
unload the current sample tube.
Cleaning the System
71.To 3 x 5mL tubes add 2 ml of FACSClean® Solution, FACSRinse®
Solution, and Milli-Q H2O.
72. Load each tube into the loading chamber.
73.Increase the flow rate to 11.0
74.Clean the tubing by running the three solutions for no less than 5 minutes
each. Repeat the same with FACSRinse® Solution for 10 min, if propidium
iodide (PI) is involved.
26