FACSAria SORP Standard Operation Protocol Basic Operation

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LKS Faculty of Medicine Version 1.1 University of Hong Kong Log In 4.Log into FACSDiva software with your own login name and password. Please contact the administrator to establish a new user account. 5.When connected, click ‘Use CST Settings’ when a mismatch was located. BD FACSAria SORP Operation Manual 4
LKS Faculty of Medicine Version 1.1 University of Hong Kong Stream Break-Off 6.The following steps are prepared for droplet formation using 100µm nozzle. 7.Turn ON the Stream (if not yet done), and allow time for the stream to pressurize and settle. 8.Adjust the Frequency (Freq) between 29 and 32 to identify the HIGHEST break-off it can generate, where the end-portion of the breaking stream can still be observed. 9.Adjust the Amplitude (Ampl) to introduce DROP 1 measured between 120 and 180; but repeat the search using a different Frequency if the finishing Amplitude value is greater than 20. 10.Fine tune the Amplitude value to stabilize the GAP measured between 8 and 12. 11.Ensure that the satellite droplet has completely merged with the trailing drop (before disappearing). 12.Match the input number with the measured value in DROP 1. 13.Allow the stream to stabilize for a minimum of 3 minutes before the activation of Sweet Spot. BD FACSAria SORP Operation Manual 5
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FACSAria SORP Standard Operation Protocol Basic Operation

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