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Development of a Bioluminescent Cell-Based Assay to ... - Promega

Development of a Bioluminescent Cell-Based Assay to ... - Promega

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Target cells can also be frozen, thaw-and-use format<br />

WIL2-S target cells can be used in frozen, thaw-and-use format<br />

Direct comparison <strong>of</strong> target cell format: continuously-cultured vs. frozen,<br />

thaw-and-use cells<br />

RLU<br />

150,000<br />

125,000<br />

100,000<br />

75,000<br />

50,000<br />

25,000<br />

Confidential and Proprietary. Not for Further Disclosure.<br />

Frozen cells<br />

Continuosly cultured cells<br />

0<br />

0.01 0.1 1 10 100 1000<br />

Final Rituximab (ng/ml)<br />

Effec<strong>to</strong>r cells: FcgRIIIa/NFAT-luc Jurkat (frozen, thaw-and use); Ab: Rituximab<br />

13

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