Development of a Bioluminescent Cell-Based Assay to ... - Promega
Development of a Bioluminescent Cell-Based Assay to ... - Promega
Development of a Bioluminescent Cell-Based Assay to ... - Promega
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Evaluating passage and cell density at freezing for<br />
WIL2-S (CD20+) Target cells<br />
Establishment <strong>of</strong> optimized<br />
frozen, thaw-and-use Target<br />
cells includes establishment<br />
<strong>of</strong> acceptable passage range<br />
and cell growth conditions<br />
prior <strong>to</strong> freezing.<br />
Important for establishing cell<br />
banks and manufacturing<br />
capabilities<br />
Confidential and Proprietary. Not for Further Disclosure.<br />
Fold induction<br />
30<br />
25<br />
20<br />
15<br />
10<br />
5<br />
EC50<br />
Pass 8<br />
Pass 12<br />
Pass 18<br />
Pass 24<br />
0<br />
0.01 0.1 1 10 100 1000<br />
Final Rituximab (ng/ml)<br />
Pass 8<br />
8.851<br />
Pass 12<br />
7.057<br />
6 hr assay<br />
Effec<strong>to</strong>r:Target ratio = 6<br />
Pass 18<br />
6.520<br />
Pass 24<br />
7.122<br />
14
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