Development of a Bioluminescent Cell-Based Assay to ... - Promega
Development of a Bioluminescent Cell-Based Assay to ... - Promega
Development of a Bioluminescent Cell-Based Assay to ... - Promega
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iological activity <strong>of</strong> therapeutic antibody preps<br />
Differentiation <strong>of</strong> blended % mixes <strong>of</strong> fully N-glycosylated & fully deglycosylated Trastuzumab<br />
RLU<br />
210 06<br />
110 06<br />
510 05<br />
210 06<br />
110 06<br />
510 05<br />
Deglycosylated 50% deglycosylated Herceptin<br />
Deglycosylated 60% deglycosylated Herceptin<br />
Deglycosylated 70% deglycosylated<br />
Herceptin<br />
unt<br />
50%unt/ 50% degly<br />
Confidential and Proprietary. Not for Further Disclosure.<br />
Deglycosylated Herceptin<br />
Deglycosylated Herceptin<br />
Deglycosylated Herceptin<br />
80% deglycosylated 90% deglycosylated 100% deglycosylated<br />
unt<br />
0<br />
-12 -10 -8<br />
log [ab], (g/ml)<br />
-6 -4<br />
EC50<br />
20%unt/80% degly<br />
unt<br />
1.720e-008<br />
20%unt/80% degly<br />
4.626e-008<br />
RLU<br />
RLU<br />
210 06<br />
110 06<br />
510 05<br />
210 06<br />
110 06<br />
510 05<br />
unt<br />
10%unt/ 90% degly<br />
0<br />
-12 -10 -8<br />
log [ab], (g/ml)<br />
-6 -4<br />
EC50<br />
unt<br />
40%unt/ 60% degly<br />
RLU Detection <strong>of</strong> small differences in Fc effec<strong>to</strong>r<br />
0<br />
-12 -10 -8<br />
log [ab], (g/ml)<br />
-6 -4<br />
EC50<br />
unt<br />
2.110e-008<br />
50%unt/ 50% degly<br />
2.990e-008<br />
0<br />
-12 -10 -8<br />
log [ab], (g/ml)<br />
-6 -4<br />
EC50<br />
unt<br />
2.082e-008<br />
unt<br />
2.037e-008<br />
40%unt/ 60% degly<br />
3.486e-008<br />
10%unt/ 90% degly<br />
7.174e-008<br />
Target cells: SKBR3; Unt = 100% glycosylated<br />
RLU<br />
RLU<br />
210 06<br />
110 06<br />
510 05<br />
210 06<br />
110 06<br />
510 05<br />
0<br />
-12 -10 -8<br />
log [ab], (g/ml)<br />
-6 -4<br />
EC50<br />
unt<br />
100% degly<br />
0<br />
-12 -10 -8<br />
log [ab], (g/ml)<br />
-6 -4<br />
EC50<br />
unt<br />
30%unt/ 70% degly<br />
unt<br />
2.002e-008<br />
unt<br />
1.988e-008<br />
30%unt/ 70% degly<br />
4.153e-008<br />
100% degly<br />
3.202e-008
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