gHK DETERMINATION OP COUMARIN 301 TOBACCO AND ...

IABLE II COTJMARIN IEVEL IN PARK DRIVE
SAMPLE 1 *
1
2
3
4
MEAN
COEFP, OP
VARIATION
3
CIGARETTES
b. 10 cigarette sample
CODMARIN
14.4
13,0
13.8
16.6
14.4
6.796
Shis measured coumarin level of 14*4 + 2.5p$/oig. agreeB veil
with the calculated level of 18ng/cig. allowing for expected
losses during flavour addition.
(ii)'COLLECTION
The smoke from 25 cigarettes containing coumarin was collected
on a Cambridge filter pad. The cigarettes were smoked to a 20mm
butt on a rotary smoking machine. The efficiency of the
Cambridge filter pad in trapping coumarin was checked by
attaching a dichloromethane trap after the pad when 25
cigarettes spiked at 500ug coumarin per cigarette were smoked.
No coumarin vaB detected in the dichloromethane solution
Indicating complete retention of coumarin on the filter.
(iii) EXTRACTION **
http://legacy.library.ucsf.edu/tid/iyl97h00/pdf
4 .
&e investigation of the extraction and clean-up stages was
undertaken using the smoke from unflavourei. Park Drive cigarettes
spiked at exaggerated levels of coumarin (>10C^g/cig.)
Hot water was found to be the best solvent for preferential
extraction of coumarin from the Cambridge filter pad, A
mixture of 2($ ether and 60% petrol (40-60 ) was found to be
suitable for extraction of coumarin from the aqueous solution
whilst removing a minimum of extraneous material. Recovery of
coumarin from the aqueous layer after 4 solvent extractions
(1 x 150, 2 x 100, 1 x 50ml) was greater than 9596.