Metabolic Disease: MILLIPLEX® RIas, and ELISa ... - Millipore

Data Sheet
Product Selection Guide
Metabolic Disease: MILLIPLEX ® MaP,
RIas, and ELISa Immunoassays
Luminex ® xMAP ® Technology

MILLIPLEX MAP
and Metabolic Syndrome
With the incidence of type 2 diabetes on the rise even among children
and teens, the study of hormones linked to metabolic syndrome is essential to
both treatment and prevention.
Obesity, diabetes and associated conditions under the umbrella of
Metabolic Syndrome are urgent health issues. Hormones produced
by various organs of the endocrine system, including adipocytes, the
pancreas and the GI tract, play an integrated role in regulating energy
and metabolism.
Inside this guide you’ll find a comprehensive list of MILLIPLEX MAP
adipokines/adipocytes, endocrine hormones, gut hormone panels, and
our new metabolic panels, based on the Luminex xMAP platform.
MILLIPLEX MAP enables you to investigate the modulation and expression
of dozens of analytes simultaneously, giving you the advantage of speed
and sensitivity, and dramatically improving productivity. And now, with
the introduction of our magnetic bead kits, you can choose the assay
type that best fits your research needs.
Metabolic disease research plays a
significant role in achieving a deeper
understanding of disease states such as:
• Metabolic Syndrome
• Diabetes
• Obesity
• Cardiovascular Disease

Available Multiplex Panels:
Adipokine / Adipocyte, page 3
• Human Adipokine Panels A and B,
and Adipocyte
• Mouse Adipokine, Adipocyte,
and Adiponectin Single-Plex
• Rat Adipokine, Adipocyte,
and Adiponectin Single-Plex
• Canine Adipokine
Food
Intake
GIP
GUT
GLP-2
GRP
PYY
Gastrin
Oxyntomodulin
VIP
CCK
Obestatin
Body
Weight
Energy
Expediture
GLP-1
Ghrelin
PANCREAS
Proinsulin
C-Peptide
Amylin
PYY3-36
Glucagon
PP
Somatostatin
Endocrine Hormone, page 8
• Human
• Mouse
• Rat
• Canine
Gut Hormone, page 13
• Human
• Mouse
• Rat
Diabetes and Obesity Biomarkers
HYPOTHALAMUS
NPY
MCH
AgRP
Orexin
Insulin Myostatin
Musclin
Adiponectin
Resistin
Leptin
HUMAN MOUSE RAT
CANINE
IGF-1, page 17
• Human
• Rat/Mouse
Metabolic Disease, page 18
• Human, Non-magnetic
Bead Format
• Human, Magnetic
Bead Format
MUSCLE
IL-6
IL-8
IL-10
TNFα
MCP-1 1
MIF
PAI-1
ASP
Adipsin n
FAT
Cortisol
HGF
NGF
VEGF
Visfatin
Vaspin
RBP4
Chemerin
Available from Millipore
1

2
MILLIPLEX MAP:
Advancing Metabolic Disease Research
Metabolic syndrome is a cluster of conditions that occur together, including
increased blood pressure, elevated insulin levels, excess body fat around the
waist and abnormal cholesterol levels. Key features of metabolic syndrome
include insulin resistance, glucose intolerance, hypertension, dyslipidemia and
central obesity—all of which are risk factors for atherosclerosis, coronary heart
disease, type 2 diabetes, kidney disease, and even premature death. Adults with
metabolic syndrome show a low-grade inflammation, whose link with obesity may
be dysregulated adipocyte production of pro- and anti-inflammatory factors.
Consequently, research done in this area covers multi-faceted fields of cytokines,
acute phase proteins, diabetes and obesity related hormones, as well as other
cardiovascular disease biomarkers.
The study results shown below, published in Physiological Research, 2006,
demonstrate a clear improvement of endothelial dysfunction markers after
rosiglitazone treatment in patients with type 2 diabetes. This finding is in
agreement with previously published studies showing that thiazolidinediones
improve the mechanical properties of the vessel wall.
Effect of PPAR-g Agonist Treatment on Markers of Endothelial Dysfunction in Patients with Type 2 Diabetes Mellitus
Serum concentrations of Plasminogen Activator Inhibitor-1 (PAI-1), soluble VCAM-1 (sVCAM-1), soluble E-Selectin (sE-Selectin),
and adiponectin in normal, diabetic, and diabetic patients treated with rosiglitazone (Avandia) for 5 months as measured using
the human CVD1 cardiovascular MILLIPLEX MAP.
R. Dolezalová, M. Haluzík, L. Bosanská, Z. Lacinová, T. Stulc, M. Haluzík, Physiological Research, November 6 (2006)

MILLIPLEX MAP Adipokine and
Adipocyte Multiplex Panels
In the past the role of white adipose tissue was thought to be limited to energy
storage and internal organ protection. The discovery of leptin secretion from
adipocytes in 1994 led to the recognition that white adipose tissue is involved
in a variety of metabolic and physiological processes. Adipocytes secrete a
number of hormones called adipokines with functions that include appetite and
energy balance, insulin sensitivity, and lipid metabolism. One of these adipokines,
adiponectin, is involved in the regulation of lipid and glucose metabolism,
influencing the body’s response to insulin. Also, its anti-inflammatory effects on
the cellular lining of blood vessel walls may help to explain the association of high
adiponectin levels with a reduced risk of heart attack.
Serum / Plasma
Samples
Cell Culture
Samples
AVAILABLE ANALYTES
Human Adipokine Panel A
Human Adipokine Panel B
Mouse Adipokine
Rat Adipokine
Canine Adipokine
Mouse Single Plex
Rat Single Plex
Human Adipocyte
Mouse Adipocyte
Rat Adipocyte
Adiponectin
HGF
*PAI-1 (active) and PAI-1 (total) cannot be plexed together.
IL-1β
IL-6
IL-8
Insulin
Leptin
MCP-1
NGF
PAI-1 (active)*
PAI-1 (total)*
Resistin
TNFα
3

4
Human Adipokine Panels A, B
(serum/plasma samples)
Human Adipocyte Panel
(cell culture samples)
PERFORMANCE SPECIFICATIONS
Precision
Intra-assay: < 8%
Inter-assay: < 21%
Specificity
Cross-reactivity between the antibodies and any of the
other analytes within each panel is non-detectable or
negligible.
Assay Conditions
• These kits may be used for the analysis of all or any
combination of analytes within a panel in serum/plasma
using the Adipokine Panels A and B and cell/tissue
extract or culture samples using the Adipocyte Panel.
• If using Adipokine Panel A with serum or plasma
samples, a 1:400 dilution is required.
• These are overnight assays requiring 25 µL sample size.
• PAI-1 active and PAI-1 total cannot be plexed together.
Analyte
Standard
Curve Range
(pg/mL)
Serum
Adipokine
Panel A
100,000 100
MILLIPLEX MAP
10,000
10 Human Adipocyte Panel
1,000
0
Standard Curves
100,000
100
0 1 10 100 1,000 10,000 100,000 1,000,000
10,000
10
1,000
0
Concentration (pg/mL)
100 0 1 10 100 1,000 10,000 100,000 1,000,000
Sensitivity (pg/mL)
10
Accuracy (%)
Concentration (pg/mL)
Serum
Adipokine
Panel B
100,000
MILLIPLEX MAP
10,000
Human Serum Adipokine Panel A
1,000 Standard Curves Adiponectin
100,000 100
10,000 10
Resistin
MILLIPLEX MAP
Human Serum Adipokine Panel A
PAI-1
1,0000
100,000
100
0
Standard Curves
100 10,000
Adiponectin
1,000,000 Resistin
10,000
10
Concentration (pg/mL) PAI-1
1,000
0
100
10
0
Adiponectin
100 10,000 1,000,000
Resistin
PAI-1
Concentration (pg/mL)
0
0 1Serum 10 100 1,000 10,000 Serum 100,000 1,000,000
Resistin
PAI-1
Adipocyte Panel Adipokine Adipokine
Concentration (pg/mL)
Panel A
Panel B
Adipocyte NGF Panel
Adiponectin 16 to 250,000 145.4 - 80.2 100.8 - 95.5
HGF 3.2 to 50,000 1.3 19.2 1.6 99.9 96.3 90.6
Insulin 3.2 to 50,000 - 2.5 1.0 - 89.2 89.1
IL-1b 0.64 to 10,000 - 0.1 0.1 - 93.9 92.1
IL-6 0.64 to 10,000 - 0.1 0.1 - 93.9 92.1
IL-8 0.64 to 10,000 - 0.1 0.1 - 93.9 92.1
Leptin 16 to 250,000 - 85.4 27.4 - 99.8 95.7
MCP-1 0.64 to 10,000 - 0.1 0.5 - 90.2 92.0
NGF 0.64 to 10,000 - 2.5 1.0 - 89.2 89.1
PAI-1 3.2 to 50,000 1.3 19.2 1.6 99.9 96.3 90.6
Resistin 3.2 to 50,000 6.7 - 4.5 100.8 - 90.2
TNFa 0.64 to 10,000 - 0.2 0.1 - 91.5 94.9
HUMAN
MFI
MFI
MFI
MFI
MFI
MFI
MFI
MFI
MFI
0
10,000 100
Human Serum Adipokine Panel A
Standard Curves
0 100 10,000 1,000,000
Concentration MILLIPLEX (pg/mL) MAP
Human Serum Adipokine Panel B
Standard Curves
100,000
MILLIPLEX MAP
10,000 Human Serum Adipokine Panel B
Standard Curves
1,000
100,000
MILLIPLEX MAP
100
10,000 Human Serum Adipokine Panel B
Standard Curves
0
100,000 1,000
0 10 100 1,000 10,000 100,000 1,000,000
Concentration (pg/mL)
1,0000
0 10 100 1,000 10,000 100,000 1,000,000
100
Concentration (pg/mL)
0
0 10 100 1,000 10,000 100,000 1,000,000
Concentration MILLIPLEX (pg/mL) MAP
Human Adipocyte Panel
Standard Curves
100,000
10,000
MILLIPLEX MAP
Human Adipocyte Panel
1,000 Standard Curves
IL-1β
IL-6
IL-8
Leptin
IL-1β TNFα
IL-6 MCP-1
IL-8 HGF
Leptin Insulin
IL-1β
TNFα NGF
IL-6
MCP-1
IL-8
HGF
Leptin
Insulin
TNFα
NGF
MCP-1
HGF
Insulin
NGF
IL-1β
IL-6
IL-8
Leptin
IL-1β TNFα
IL-6 MCP-1
IL-8 HGF
Leptin Adiponectin
IL-1β
TNFα Resistin
IL-6
MCP-1 PAI-1
IL-8
HGF NGF
Leptin
Adiponectin
TNFα
Resistin
MCP-1
PAI-1
HGF
NGF
Adiponectin

Mouse Adipokine Panel
(serum/plasma samples)
Mouse Adipocyte Panel
(cell culture samples)
Mouse Adiponectin
Single Plex
PERFORMANCE SPECIFICATIONS
Standard Curve Ranges
Adipokine Panel and
Adiponectin Single Plex: 16 to 50,000 pg/mL
Adipocyte Panel: 3.2 to 10,000 pg/mL
Precision
Intra-assay: < 5%
Inter-assay: < 12%
Specificity
Cross-reactivity between the antibodies and any of the
other analytes within each panel is non-detectable or
negligible.
Assay Conditions
• These kits may be used for the analysis of all or any
combination of analytes within a panel in serum/plasma
using the Adipokine Panel and cell/tissue extract or
culture samples using the Adipocyte Panel.
• The measurement of adiponectin in serum or plasma
requires a 1:5000 dilution because the circulating levels
of this protein are at a µg/mL range.
• This is an overnight assay requiring 10 µL sample size.
Analyte
100,000
100
MILLIPLEX MAP
10,000
10 Mouse Serum Adipokine
1,000
1
100,000
100 10
Standard Curves
100 1,000 10,000 100,000
10,000
10
1,000
1
Concentration (pg/mL)
100 10 100 1,000 10,000 100,000
Sensitivity (pg/mL)
1 10 100 1,000 10,000
Accuracy (%)
100,000
Concentration (pg/mL)
Serum Adipokine Panel Adipocyte Panel Serum Adipokine Panel Adipocyte Panel
Adiponectin - 2.6 - 104
Insulin 42.2 - 95 -
IL-6 1.9 1.9 105 106
Leptin 16.7 16.8 101 109
MCP-1 14.4 9.7 99 106
PAI-1 16.4 10.8 105 106
Resistin 1.8 1.9 104 106
TNFa 4.4 2.8 95 96
MFI
MFI
MFI
100,000
10,000
MFI
MFI
MFI
MFI
MFI
MFI
1,000
10
1
10 100 1,000 10,000 100,000
Concentration MILLIPLEX (pg/mL) MAP
Mouse Adipocyte
Standard Curves
100,000
10,000
MILLIPLEX MAP
Mouse Adipocyte
1,000 Standard Curves
100,000
100 MILLIPLEX MAP
10,000
10
1,000
0
100,000
100
0
Mouse Adipocyte
Standard Curves
10 100 1,000 10,000 100,000
10,000
10
1,000
0
Concentration (pg/mL)
100 0 10 100 1,000 10,000 100,000
10 Concentration (pg/mL)
0
0 10 100 1,000 10,000 100,000
Concentration MILLIPLEX (pg/mL) MAP
Mouse Adiponectin Single Plex
Standard Curves
10,000
1,000
MILLIPLEX MAP
Mouse Adiponectin Single Plex
Standard Curves
10,000
100 MILLIPLEX MAP
Mouse Adiponectin Single Plex
1,000
10
Standard Curves
10,000
1 10 100 1,000 10,000 100,000
100
Concentration (pg/mL)
1,000
10
100
1 10 100 1,000 10,000 100,000
Concentration (pg/mL)
10
Mouse Serum Adipokine
Standard Curves
MILLIPLEX MAP
Mouse Serum Adipokine
Standard Curves
Concentration (pg/mL)
Insulin
MCP-1
Leptin
IL-6
TNFα Insulin
PAI-1 MCP-1
Resistin Leptin
IL-6
Insulin
TNFα
MCP-1
PAI-1
Leptin
Resistin
IL-6
TNFα
PAI-1
Resistin
MCP-1
Leptin
IL-6
TNFα
PAI-1 MCP-1
Resistin Leptin
Adiponectin
IL-6
TNFα
MCP-1
PAI-1
Leptin
Resistin
IL-6
Adiponectin
TNFα
PAI-1
Resistin
Adiponectin
MOUSE
5

6
Rat Adipokine Panel
(serum/plasma samples)
Rat Adipocyte Panel
(cell culture samples)
Rat Adiponectin Single Plex
PERFORMANCE SPECIFICATIONS
Precision
Adipocyte Panel (in buffer)
Intra-assay: < 8% Inter-assay: < 20%
Adipokine Panel (in matrix)
Intra-assay: < 4% Inter-assay: < 14%
Adiponectin Single Plex (in buffer)
Intra-assay: < 5% Inter-assay: < 13%
Specificity
Cross-reactivity between the antibodies and any of the
other analytes within each panel is non-detectable or
negligible.
Assay Conditions
• These kits may be used for the analysis of all or any
combination of analytes within a panel in serum/plasma
using the Adipokine Panel and cell/tissue extract or
culture samples using the Adipocyte Panel.
• The measurement of adiponectin in serum or plasma
requires a 1:500 dilution because the circulating levels
of this protein are at a µg/mL range.
• This is an overnight assay requiring 25 µL sample size.
• PAI-1 active and PAI-1 total cannot be plexed together.
10 100 1,000 10,000 100,000 1,000,000
Standard
Sensitivity* (pg/mL) Concentration (pg/mL) Accuracy (%)
Analyte Curve Range
MILLIPLEX MAP
(pg/mL)
Adipocyte Adipokine Adiponectin Adipocyte Canine Adipokine Adipokine Panel Adiponectin
Adiponectin
IL-1b
IL-6
Insulin
Leptin
MCP-1
PAI-1 (active)
PAI-1 (total)
TNFa
122 to 50,000
4.9 to 20,000
12.2 to 50,000
12.2 to 50,000
12.2 to 50,000
4.9 to 20,000
12.2 to 50,000
12.2 to 50,000
4.9 to 20,000
(in buffer)
32.7
0.9
3.4
-
2.5
10.5
14.7
12.5
1.1
(in serum)
-
1.2
8.8
52.5
9.7
25.8
31.6
30.7
3.2
(in buffer)
100,000
40.7
10,000
-
100,000
1,000
-
10,000
100
-
100,000 1,000
10
-
10,000 100
1
-
1,000 10
-
1001
-
10
-
1
1
(in buffer) Standard Curves (in serum) (in buffer)
MILLIPLEX MAP
Canine
99
Adipokine Panel
- 95
Standard Curves
96MILLIPLEX MAP 103 -
Canine Adipokine Panel
89 106 -
Standard Curves
Resistin
- 107 Adiponectin -
97 103 Resistin -
99 106 Adiponectin -
10100 1,000 10,000 100,000 1,000,000
92 110 Resistin -
Concentration (pg/mL)
117 98 Adiponectin -
10100 1,000 10,000 100,000 1,000,000
Concentration 99 (pg/mL) 116 -
1
* Minimum Detectable Concentrations
1 10100 1,000 10,000 100,000 1,000,000
RAT
MFI
MFI
MFI
100,000
10,000
100,000
1,000
10,000
100,000 100
1,000
10,000 10
100
1
1,000
MFI
MFI
MFI
MFI
MFI
MFI
MFI
MFI
MFI
10
100 1
10
100,000
1
10,000
100,000
1,000
10,000
100,000 100
1,000
10,000 10
100
1
1,000
10
100 1
10
1
MILLIPLEX MAP
Rat Adipokine Panel
Standard Curves
MILLIPLEX MAP
Insulin
Rat Adipokine Panel
MCP-1
Standard Curves
Leptin
MILLIPLEX MAP
Insulin
Rat Adipokine Panel
IL-1β
MCP-1
Standard Curves
IL-6
Leptin
Insulin TNFα
IL-1β
MCP-1 PAI-1
IL-6
Leptin
TNFα
10 100 1,000 10,000 100,000 IL-1β
PAI-1
Concentration (pg/mL)
IL-6
TNFα
10 100 1,000 10,000 100,000
PAI-1
Concentration (pg/mL)
10 100 1,000 10,000 100,000
Concentration (pg/mL)
MILLIPLEX MAP
Rat Adipocyte Panel
Standard Curves
MILLIPLEX MAP
MCP-1
Rat Adipocyte Panel
Leptin
Standard Curves
IL-1β
MILLIPLEX MAP
MCP-1
IL-6
Rat Adipocyte Panel
Leptin
TNFα
Standard Curves
IL-1β
MCP-1 PAI-1
IL-6
Leptin Adiponectin
TNFα
IL-1β
PAI-1
IL-6
10 100 1,000 10,000 100,000 1,000,000 Adiponectin
TNFα
Concentration (pg/mL)
PAI-1
10 100 1,000 10,000 100,000 1,000,000
Adiponectin
Concentration (pg/mL)
10 100 1,000 10,000 100,000 1,000,000
Concentration (pg/mL)
MILLIPLEX MAP
Rat Adiponectin Single Plex
Standard Curves
MILLIPLEX MAP
10,000
Rat Adiponectin Single Plex
Standard Curves
1,000
10,000
100
1,000
10,000 10
100
1,000 1
MILLIPLEX MAP
Rat Adiponectin Single Plex
Standard Curves
10 10 100 1,000 10,000 100,000 1,000,000
100
1
Concentration (pg/mL)
10 10 100 1,000 10,000 100,000 1,000,000
Concentration (pg/mL)
1
Concentration (pg/mL)

Canine Adipokine Panel
PERFORMANCE SPECIFICATIONS
Precision
Intra-assay: < 10% Inter-assay: < 17%
Specificity
Cross-reactivity between the antibodies is non-detectable
or negligible.
Assay Conditions
• These kits may be used for the analysis of all or any
combination of analytes within a panel in serum/plasma
using the Adipokine Panel and cell/tissue extract or
culture samples using the Adipocyte Panel.
• The measurement of adiponectin and resistin in serum
or plasma requires a 1:500 dilution.
• The user must determine the appropriate dilutions of
cell culture supernatant.
• This is an overnight assay requiring 25 µL sample size
Analytes Standard Curve Range (pg/mL) Sensitivity* (pg/mL) Accuracy (%)
Adiponectin 32 to 500,000 22.1 115
Resistin 3.2 to 50,000 1.5 116
* Minimum Detectable Concentrations
MFI
100,000
10,000
1,000
100
10
Concentration (pg/mL)
MILLIPLEX MAP
Canine Adipokine Panel
Standard Curves
1
1 10100 1,000 10,000 100,000 1,000,000
Concentration (pg/mL)
Resistin
Adiponectin
CANINE
7

8
MILLIPLEX MAP
Endocrine Multiplex Panels
Hormones produced by various organs of the endocrine system, including
adipocytes, the pancreas and the GI tract, play an integrated role in regulating
energy and metabolism. These key hormones are linked to obesity and
diabetes and may be involved in the pathogenesis of metabolic disorders.
Leptin, a gatekeeper hormone produced by adipocytes, regulates appetite
and metabolism. Further downstream, amylin, glucagon and insulin are key
glycemic control hormones produced by the pancreas, and are regulated by
gut hormones such as GLP-1. C-peptide, a byproduct of insulin production,
converts metabolic signals to nitric oxide signaling, and is a marker of diabetes.
AVAILABLE ANALYTES
Human
Mouse
Rat
Canine
Amylin* (active)
Amylin* (total)
C-Peptide
GLP-1 (active)
Glucagon
*Amylin (active) and Amylin (total) cannot be plexed together.
Insulin
Leptin

Human Endocrine Panel
PERFORMANCE SPECIFICATIONS
Standard Curve Range
6.2 to 4,500 pM for all analytes
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
Assay Conditions
• Amylin (active) and Amylin (total) cannot be plexed
together.
• This kit may be used for the analysis of either active
amylin or total amylin with any or all of the analytes in
the panel in serum, plasma, other body fluids, and cell/
tissue extract or culture samples.
• This is an overnight assay requiring 25 µL sample size.
STANDARD UNIT COMPARISON
Hormone Molecular Weight 1,000 pM
Glucagon
ng/mLInsulin
Amylin 3,903
100
100
GLP-1 (active)
0.39
C-Peptide 3,617 100 0
0.36
GLP-1 (active) 3,297
1
100
10 100 1,000 10,000
0.33
Glucagon 3,483 100
Concentration (pM)
0.34
Insulin 5,808 100 0.58
Leptin 16,027 100 1.60
Precision
Hormone Sensitivity MinDC (pM)
MILLIPLEX MAP Accuracy (%)
Intra-Assay (%CV) Inter-Assay Rat Endocrine (%CV) Panel
Standard Curves
Amylin* (active) 1.5 3.8 100,000 8.4 80
Amylin* (total) 2.3 4.7 10,000 9.3
Amylin (active)
94
Leptin
C-Peptide 2.7 7.3 1,000 4.2 Glucagon 84
GLP-1 (active) 3.4 5.1 100 7.4 136 Insulin
Glucagon 2.2 4.9
10
7.1
GLP-1 (active)
93
Insulin 3.0 2.6
1
7.2 83
Leptin 8.7 5.1 1 7.4 10 100 1,000 10,000 103
* Amylin (active) and Amylin (total) cannot be plexed together.
Concentration (pM)
MFI
MFI
MFI
MFI
100,000
10,000
1,000
100
10,000
1 10 100 1,000 10,000
Concentration (pM)
MILLIPLEX MAP
Mouse Endocrine Panel
Standard Curves in Serum Matrix
100,000
100,000
10,000
1,000
100
10
MILLIPLEX MAP
Human Endocrine 6-plex Assay
Standard Curves
MILLIPLEX MAP
Canine Endocrine Panel
Standard Curves
Amylin (active)
Leptin
Glucagon
GLP-1 (active)
C-Peptide
Insulin
Amylin (active)
Leptin
Leptin
HUMAN
Glucagon
Insulin
GLP-1 (active)
9

10
Mouse Endocrine Panel
PERFORMANCE SPECIFICATIONS
Standard Curve Range
6.2 to 4,500 pM for all analytes
Precision
Intra-assay: 3.8 – 10.6%
Inter-assay: 4.8 – 20.7%
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
Assay Conditions
• This kit may be used for the analysis of all or any
combination of the analytes in this panel in serum,
plasma, other body fluids, and cell/tissue extract or
culture sample.
• This is an overnight assay requiring 10 µL sample size.
STANDARD UNIT COMPARISON
1,000
Glucagon
Hormone Molecular Weight pM
100
ng/mLInsulin
GLP-1 (active)
Amylin 3,921 10 100 0.58
GLP-1 (active) 3,297 100 1
0.33
Glucagon 3,483 1001 10 100 1,000 10,0000.35
Insulin 5,800 100
Concentration (pM)
0.58
Leptin 16,162 100 1.60
Hormone Sensitivity MinDC (pM)
Accuracy (%)
Serum Matrix (%) MILLIPLEX MAP Serum Samples (%)
Amylin (active) 6.2 101
Canine Endocrine Panel
Standard Curves 80
GLP-1 (active) 6.2 100,000 101 115
Leptin
Glucagon 6.2 10,000 99.7 106
Glucagon
Insulin 55.6 1,000 100 106Insulin
Leptin 6.2 100 101 97GLP-1
(active)
MOUSE
MFI
MFI
MFI
MFI
10,000
1,000
100
10,000
0
1 10 100 1,000 10,000
Concentration (pM)
MILLIPLEX MAP
Mouse Endocrine Panel
Standard Curves in Serum Matrix
100,000
1,000
100
100,000
10,000
10
1 10 100 1,000 10,000
Concentration (pM)
MILLIPLEX MAP
Rat Endocrine Panel
Standard Curves
1
1 10 100 1,000 10,000 100,000
Concentration (pM)
Glucagon
GLP-1 (active)
C-Peptide
Insulin
Amylin (active)
Leptin
Glucagon
Insulin
GLP-1 (active)
Amylin (active)
Leptin

Rat Endocrine Panel
PERFORMANCE SPECIFICATIONS
Standard Curve Range
Amylin (active): 17 to 18,000 pM
GLP-1 (active): 23 to 23,000 pM
Glucagon: 15 to 6,000 pM
Insulin: 7 to 10,000 pM
Leptin: 98 to 7,000 pM
Precision
Intra-assay: 3.8 – 10.6%
Inter-assay: 4.8 – 20.7%
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
Assay Conditions
• This kit may be used for the analysis of all or any
combination of the analytes in this panel in serum,
plasma, other body fluids, and cell/tissue extract or
culture samples.
• This is an overnight assay requiring 10 µL sample size.
STANDARD UNIT COMPARISON
Concentration (pM)
Hormone Molecular Weight pM ng/mL
Amylin 3,921 100 0.58
GLP-1 (active) 3,297 100 0.33
Glucagon 3,483 100 0.35
Insulin 5,800 100 0.58
Leptin 16,162 100 1.60
Hormone
Sensitivity Accuracy (%)
MinDC (pM) Serum Matrix (%)
Amylin (active) 6.2 101
GLP-1 (active) 6.2 101
Glucagon 6.2 99.7
Insulin 55.6 100
Leptin 6.2 101
MFI
MFI
100
0
100,000
10,000
1,000
100
10
1
100,000
10,000
1,000
100
10
1 10 100 1,000 10,000
Concentration (pM)
MILLIPLEX MAP
Rat Endocrine Panel
Standard Curves
1 10 100 1,000 10,000
Concentration (pM)
MILLIPLEX MAP
Canine Endocrine Panel
Standard Curves
1
1 10 100 1,000 10,000 100,000
GLP-1 (active)
Amylin (active)
Leptin
Glucagon
Insulin
GLP-1 (active)
Leptin
Glucagon
Insulin
GLP-1 (active)
RAT
11

12
Canine Endocrine Panel
PERFORMANCE SPECIFICATIONS
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
Assay Conditions
• This kit may be used for the analysis of all or any
combination of the analytes in this panel in serum,
plasma, other body fluids, and cell/tissue extract or
culture samples.
• The assay requires a 1:3 dilution in serum or plasma
using assay buffer.
• Users will need to determine the appropriate dilution
when using cell or tissue culture supernatants.
• This is an overnight assay requiring 25 µL sample size.
Hormone
Standard Curve
Range (pg/mL)
Sensitivity MinDC
(pg/mL)
Precision
Intra-Assay (%CV) Inter-Assay (%CV)
Accuracy (%)
GLP-1 (active) 20.6 to 15,000 12.1 5.6 14.6 73.7
Glucagon 5.5 to 4,000 5.1 10.2 17.8 96.4
Insulin 137.2 to 100,000 73.0 4.7 13.4 81.4
Leptin 137.2 to 100,000 148.0 4.6 17.6 55.1
CANINE
MFI
10
1
100,000
10,000
1,000
100
10
1 10 100 1,000 10,000
Concentration (pM)
MILLIPLEX MAP
Canine Endocrine Panel
Standard Curves
1
1 10 100 1,000 10,000 100,000
Concentration (pM)
GLP-1 (active)
Leptin
Glucagon
Insulin
GLP-1 (active)

MILLIPLEX MAP Gut
Hormone Multiplex Panels
In the study of obesity, advances are unfolding at a rapid pace, providing
new hope for unraveling and potentially eradicating this disease. What we
know about obesity has been transformed by the identification of several
gut hormones or peptide hormones. While much remains unknown about
the specific role they play in the cause of obesity, we do know that these
peptides play a significant role in the regulation of gut motility and secretion,
pancreatic islet hormone secretion, food intake and energy expenditure.
In addition evidence of their presence and synthesis may be detectable in
other organs of the body, including the brain where they may function as
neuropeptides, taking part in both neurotransmission and neuromodulation.
AVAILABLE ANALYTES
Human
Mouse
Rat
Amylin* (active)
Amylin* (total)
Ghrelin (active)
GIP (total)
GLP-1 (active)
*Amylin (active) and Amylin (total) cannot be plexed together.
Insulin
Leptin
PP
PYY (total)
13

Ghrelin (pg/mL) Ghrelin (pg/mL)
Leptin (pg/mL Leptin x 1000) (pg/mL x 1000)
Amylin (pg/mL) Amylin (pg/mL)
PP (pg/mL)
PP (pg/mL)
300
250
200
300
150
250
100
200
50
150
0
100
250
200
14
50
0
16
14
12
10
16
14
8
6
12
4
10
2
8
0
6
150
250
100 200
10
150
0
100
10
140
120
100
0
140 80
120 60
100 40
20 80
60 0
40
20
0
4
2
0
120
14
Serum Ghrelin Serum Ghrelin
100
300
80
250
60
200 Serum Ghrelin Serum Ghrelin
40
300
15020
250
1000
200 Fast 1
50
150
0
Fast 100 1Fast
Post 1
Serum 12 AmylinSerum
GIPSerum
GIP
Figure 1. 140 Gut Hormone 140Levels
Before and After Meal
10
120
250
120 8 120
100
200
100 6 100Serum
GIP Serum GIP
80
150
140 80 4 80 140
250
2
60
120 60
60 120
100
0
200 40
100
Post
40
40 100
Fast 1 Post
10
20 80
2080
150 20
0
60 0
060
0
100
Post Fast Fast Fast 1
1
40
1
40
Post Post Post
50
20
20
10
Ghrelin (pg/mL)
Ghrelin (pg/mL) GIP (pg/mL)
Fast
Leptin (pg/mL
GLP-1
x 1000)
Leptin (pg/mL x 1000)
(pg/mL)
40
Serum Leptin Serum Leptin
16
30
14
12 20
Serum Leptin Serum Leptin 100
10
10
16
80
8
14
60
60
12
40
4 Fast 1
Post
10
2
20
8
0
0
6
Fast Fast 1 Post 1 Post
4
Amylin (pg/mL)
Amylin (pg/mL) PYY (pg/mL)
PP (pg/mL)
Insulin (pg/mL x 1000)
140
Fast
0
50
120
100
Serum Amylin Serum Amylin
80
250
60
200
Serum 40 Amylin Serum Amylin
150
250 20
PP (pg/mL)
100 2000
10
150
10
0
2.5
2
0
Serum GIP
1 Fast Post 1
Serum GLP-1
1Fast
Post 1
Serum PYY
Fast
0
100
Fast 1Fast
Fast
Post 1
1 Fast Post 1
Serum GIP Correlation Insulin
Serum 2 PP Serum PP
140
1.5
120
100
1
Serum PP
80 140
.5
60 120
Serum PP
0
40 100
2080
Fast 1
060
Fast 40
Fast
20
0
1
Post
Post
Post
Post
Leptin Correlation
Fast 1 Post 1 Post
1Fast
Post 1
Amylin (pg/mL)
PP (pg/mL)
140
120
Post
GIP (pg/mL)
GIP (pg/mL)
0
Fast 1Fast
Serum Amylin
200 Serum PPSerum
GLP-1 Serum GLP-1
50
50
150
GLP-1 (pg/mL)
GLP-1 (pg/mL)
40
Serum GLP-1 Serum GLP-1
3050
2040
Fast
1030
020
Post 1Fast
Post 1
80 Serum PYYSerum
PYY
120
60
100
40
Fast 80
20
60
0
1 Post
Fast 40 1Fast
Post 1
PYY (pg/mL)
PYY (pg/mL)
Insulin (pg/mL x 1000)
Insulin (pg/mL x 1000)
Serum PYYSerum
PYY
120
100
10
Serum Insulin Serum Insulin
2.5
2
Serum Insulin Serum Insulin
1.52.5
1 2
.51.5
Ghrelin 0 1Correlation
Fast 1Fast
Post 1
Post 1
Post 1
Post
Post
0
Post
Post
20
20
Figure 2. Gut Hormone Correlations MILLIPLEX MAP vs. ELISA
0
0
Post
Fast 1 Fast Post 1
Post
Insulin Correlation
Post
GIP (pg/mL) GIP (pg/mL)
Leptin (pg/mL x 1000)
GLP-1 (pg/mL) GLP-1 (pg/mL) Amylin (pg/mL)
Fast
PYY (pg/mL) PP (pg/mL)
PYY (pg/mL)
Insulin (pg/mL Insulin x 1000) (pg/mL x 1000)
0
250
16
40
100
30
10 50
20 40 0
10 30
20 0
10
140
120
0
100
120
80
100
60
80
40
120
20 60
100
40 0
80
20
60
0
40
2.5
2
1.5 2.5
12
1.5 .5
01
.5
0
1 Fast
Serum Leptin
.5
0
1
0
Fast 1 Fast
Serum PP
Fast
1 Fast
Post
Post 1
Amylin (pg/mL) PYY (pg/mL)
PP (pg/mL) Insulin (pg/mL x 1000)
Lep
Post
Post
0
2.5
2
140 1.5
120
1
100
.5
80
600
40
20
2
0
Fast
GLP-1 (pg/mL)
Fast
Fast
50
40
Serum PYY
30
20
Serum Amylin
10
0
Fast
100
Serum Insulin
80
PYY (pg/mL)
Fast
Fast
Insulin (pg/mL x 1000)
120
60
Serum 40 PP
20
0
2.5
2
1.5
1
.5
0
1
1
1
1
1
PP Correlation
PYY Correlation
Fast
Fast
Post
Serum GLP-1
1
Post
Post
Serum PYY
1
Post
Post
Serum Insulin
Figures 1 and 2. Assay was validated with fasting and postprandial in normal human serum and plasma samples (n=20). Our data showed
significant meal-stimulated responses of circulating gut hormones as expected. The sample values generated with the multiplex assay
correlated well with those generated using commercially available ELISA kits.
1
Post
Post
Post

Human Gut Hormone Panel
PERFORMANCE SPECIFICATIONS
Precision
Intra-assay: < 11%
Inter-assay: < 19%
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
Assay Conditions
• Amylin (active) and Amylin (total) cannot be plexed
together.
• This kit may be used for the analysis of either active
amylin or total amylin with any or all of the analytes in
the panel in serum, plasma, other body fluids, and cell/
tissue extract or culture samples.
• This is an overnight assay requiring 25 µL sample size.
MILLIPLEX MAP
Mouse Gut Hormone Panel
Standard Curves
Ghrelin (active)
Hormone Standard Curve Range (pg/mL)
100,000
Sensitivity MinDC (pg/mL)
10,000
Leptin
Accuracy (%)
GIP (total)
Amylin* (active) 27.4 to 20,000 31.5 90 GLP-1 (active)
Amylin* (total) 27.4 to 20,000
1,000
3.2
Amylin (active)
93
PP
Ghrelin (active) 13.7 to 10,000 1001.8
85 PYY (total)
GIP (total) 2.7 to 2,000 0.2
10
89 Insulin
GLP-1 (active) 13.7 to 10,000 5.2 1 10 100 1,000 10,000 100,000 83
Insulin 137 to 100,000 44.5 Concentration (pg/mL)
85
Leptin 137 to 100,000 2.4 102
PP 13.7 to 10,000 2.4 88
PYY (total) 13.7 to 10,000 8.4 107
ANALYTES
Human
Mouse
Rat
Canine**
Amylin* (active)
Amylin* (total)
Ghrelin (active)
GIP (total)
GLP-1 (active)
*Amylin (active) and Amylin (total) cannot be plexed together.
**Coming Soon
Insulin
Leptin
MFI
MFI
MFI
PP
100,000
10,000
1,000
100
10
100,000
PYY (total)
10,000
1,000
100
MILLIPLEX MAP
Human Gut Hormone Panel
Standard Curves
1 10 100 1,000 10,000 100,000 1,000,000
Concentration (pg/mL)
MILLIPLEX MAP
Rat Gut Hormone Panel
Standard Curves
Glucagon
10
1 10 100 1,000 10,000 100,000
Concentration (pg/mL)
Ghrelin (active)
Leptin
GIP (total)
GLP-1 (active)
Amylin (active)
PP
PYY (total)
Insulin
Ghrelin (active)
Leptin
GIP (total)
GLP-1 (active)
Amylin (active)
PP
PYY (total)
Insulin
HUMAN
15

16
Mouse Gut Hormone Panel
Rat Gut Hormone Panel
PERFORMANCE SPECIFICATIONS
Precision
Intra-assay: < 7%
Inter-assay: < 23%
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
Assay Conditions
• These kits may be used for the analysis of all or any
combination of the analytes in this panel in serum,
plasma, other body fluids, and cell/tissue extract or
culture samples.
• These overnight assays requiring 25 µL sample size.
MOUSE
Hormone
RAT
Standard Curve
Range (pg/mL)
Sensitivity MinDC
(pg/mL)
MFI
MFI
MFI
MFI
100,000
10,000 10,000
1,000 1,000
100 100
Concentration (pg/mL)
10 10
1 1 10 10 100 100 1,000 1,000 10,000 10,000 100,000
Concentration (pg/mL)
100,000
10,000 10,000
1,000 1,000
100 100
MILLIPLEX MAP MAP
Mouse Gut Gut Hormone Panel
Standard Curves
MILLIPLEX MAP MAP
Rat Rat Gut Gut Hormone Panel
Standard Curves
10 10
1 1 10 10 100 100 1,000 1,000 10,000 10,000 100,000
Concentration (pg/mL)
Accuracy (%)
Ghrelin (active)
Leptin
GIP GIP (total)
GLP-1 (active)
Amylin (active)
PP PP
PYY PYY (total)
Insulin
Ghrelin (active)
Leptin
GIP GIP (total)
GLP-1 (active)
Amylin (active)
PP PP
PYY PYY (total)
Insulin
Mouse Rat Mouse Rat
Amylin (active) 41 to 30,000 7 20 96 92
Ghrelin (active) 7 to 5,000 2 2 91 90
GIP (total) 3 to 2,000 1 1 91 90
GLP-1 (active) 41 to 30,000 28 28 85 84
Insulin 69 to 50,000 25 28 92 93
Leptin 69 to 50,000 22 27 90 87
PP 7 to 5,000 17 17 84 88
PYY (total) 7 to 5,000 16 16 84 86

MILLIPLEX MAP Human and
Rat/Mouse IGF-1 Single Plexes
Insulin-like Growth Factor (IGF-1) is a polypeptide endocrine
hormone similar in structure to insulin. Stimulated by growth
hormone and produced primarily in the liver, it binds to the
IGF-1 receptor with a high affinity and to the insulin receptor
with a much lower affinity. The tyrosine kinase IGF-1 receptor
is a potent activator of the Akt signaling pathway, which drives
a variety of processes including cell growth and division and
apoptosis inhibition. Because approximately 98% of IGF-1 is
always bound to one of six binding proteins (IGF-BP), extraction
is required to measure extracellular protein concentrations.
Increased levels of IGF-1 have been correlated with an increased
risk of cancer. In addition, while involved in reducing hemoglobin
A1C levels and daily insulin consumption in types 1 and 2
diabetes, these increased levels have been also implicated in the
development of diabetic retinopathy. Drugs have been developed
as replacement therapy for severe primary IGF-1 deficiency, a
group of diseases in which patients do not respond to growth
hormone therapy.
PERFORMANCE SPECIFICATIONS
Standard Curve Range
Human: 137 to 100,000 pg/mL
Rat/Mouse: 3.2 to 10,000 pg/mL
Sensitivity
Human: 60 pg/mL
Rat/Mouse: 3.2 pg/mL
Accuracy
Human: 84%
Rat/Mouse: 99%
Precision
Human
Intra-assay: < 5% Inter-assay: < 12%
Rat/Mouse
Intra-assay: < 10% Inter-assay: < 5%
Specificity
The Ab pair used in each assay is specific and displays
no cross-reactivity to insulin or IGF-2.
Assay Conditions
Human
MFI
MFI
MFI
MFI
10,000
1,000
10,000 100
1,000 10
100
10
100,000
10,000
1,000
100,000
100
100 1,000
Concentration (pg/mL)
10,000 100,000
10,000
10
1,000 1 10 100 1,000 10,000 100,000
100
MILLIPLEX MAP
Human IGF-1 Single Plex
Standard Curve
MILLIPLEX MAP
Human IGF-1 Single Plex
Standard Curve
100 1,000
Concentration (pg/mL)
MILLIPLEX MAP
Rat/Mouse IGF-1 Single Plex
Standard Curve
10
1 10 100 1,000 10,000 100,000
Concentration (pg/mL)
10,000 100,000
MILLIPLEX MAP
Rat/Mouse IGF-1 Single Plex
Standard Curve
Concentration (pg/mL)
• This assay may be used for the analysis human IGF-1 in
serum, plasma, other body fluids, and cell/tissue extract
or culture samples.
• Sample extraction is required for the measurement of
IGF-1 in serum or plasma.
• This is an overnight assay requiring 25 µL sample size.
Rat/Mouse
• This assay may be used for the analysis of rat/mouse
IGF-1 in serum, plasma, other body fluids, and cell/tissue
extract or culture samples.
• This single plex requires a 1:200 dilution of 25 µL of
serum or plasma in assay buffer. Users need to
determine appropriate dilutions in cell/tissue extracts or
culture samples.
• This is an overnight assay requiring 25 µL sample size.
17

18
MILLIPLEX MAP Metabolic
Multiplex Panels
Key features of metabolic syndrome include insulin resistance, glucose
intolerance, hypertension, dyslipidemia, central obesity and low-grade inflammation.
These features are risk factors for atherosclerosis, coronary heart disease, type
2 diabetes, kidney disease and premature death. Given the diverse manifestations
and long-term effects of metabolic syndrome, a comprehensive study of this disease
category requires reliable quantitation of cytokines, acute phase proteins, diabetes
and obesity related hormones, as well as other cardiovascular disease biomarkers.
The MILLIPLEX MAP Metabolic Multiplex Panels contain the most comprehensive range
of analytes for studying metabolic pathways across multiple organ systems.
AVAILABLE ANALYTES
Human Non-Magnetic Bead Format
Human Magnetic Bead Format
Amylin* (active)
Amylin* (total)
*Amylin (active) and Amylin (total) cannot be plexed together.
C-peptide
Ghrelin (active)
GIP (total)
GLP-1 (active)
Glucagon
IL-6
Insulin
Leptin
MCP-1
PP
PYY
TNFα

Human Metabolic Hormone Panel (Non-Magnetic Bead)
PERFORMANCE SPECIFICATIONS
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
Assay Conditions
• Overnight assay requiring 25 µL sample size.
• No sample dilution is required for serum and plasma.
• See kit protocol for instructions regarding magnetic
bead applications.
Analyte
Standard Curve
Range (pg/mL)
Sensitivity
(pg/mL)
Precision
Intra-Assay (%CV) Inter-Assay (%CV)
Accuracy (%)
Amylin (active) 27 to 20,000 11.0 < 7 < 13 69
C-Peptide 69 to 50,000 20.0 < 4 < 8 94
Ghrelin (active) 14 to 10,000 3.0 < 4 < 7 92
GIP (total) 3 to 2,000 0.7 < 5 < 7 87
GLP-1 (active) 14 to 10,000 20.0 < 3 < 11 79
Glucagon 14 to 10,000 10.0 < 5 < 10 70
IL-6 14 to 10,000 2.4 < 7 < 13 82
Insulin 137 to 100,000 50.0 < 4 < 8 97
Leptin 137 to 100,000 160.0 < 9 < 8 74
MCP-1 14 to 10,000 2.4 < 5 < 11 85
PP 14 to 10,000 5.0 < 4 < 9 91
PYY 14 to 10,000 19.0 < 4 < 12 91
TNFa 3 to 2,000 0.6 < 5 < 13 86
MFI
100,000
10,000
1,000
100
10
MILLIPLEX MAP
Human Metabolic Hormone
Non-Magnetic Beads Panel
Standard Curves
1 10 100 1,000 10,000 100,000
Concentration (pg/mL)
Amylin (active)
C-Peptide
Ghrelin (active)
GIP (total)
GLP-1 (active)
Glucagon
IL-6
Insulin
Leptin
MCP-1
PP
PYY
TNFα
HUMAN
19

20
Human Metabolic Hormone Panel (Magnetic Bead)
PERFORMANCE SPECIFICATIONS
Specificity
Cross-reactivity between the antibodies and any of the
other analytes in this panel is non-detectable or negligible.
Assay Conditions
• Overnight assay requiring 25 µL sample size.
• No sample dilution is required for serum and plasma.
• Assay requires a magnetic bead plate washer
(Alternatively, you can use Millipore filter plates with a
vacuum manifold, Catalogue No. MSVMHTS00)
Analyte
Standard Curve
Range (pg/mL)
Sensitivity (pg/mL)
Precision
Intra-Assay (%CV) Inter-Assay (%CV)
Accuracy (%)
Amylin (active) 27 to 20,000 8.0 < 3 < 21 81
C-Peptide 69 to 50,000 4.0 < 8 < 16 83
Ghrelin (active) 14 to 10,000 2.0 < 3 < 7 92
GIP (total) 3 to 2,000 0.4 < 4 < 10 91
GLP-1 (active) 14 to 10,000 12.0 < 2 < 13 81
Glucagon 14 to 10,000 5.0 < 3 < 11 79
IL-6 14 to 10,000 1.6 < 7 < 17 90
Insulin 137 to 100,000 35.0 < 3 < 10 90
Leptin 137 to 100,000 138.0 < 7 < 10 78
MCP-1 14 to 10,000 2.0 < 3 < 11 84
PP 14 to 10,000 3.0 < 6 < 9 92
PYY 14 to 10,000 22.0 < 7 < 8 94
TNFa 3 to 2,000 0.2 < 3 < 12 97
HUMAN
MFI
100,000
10,000
1,000
100
10
MILLIPLEX MAP
Human Metabolic Hormone
Magnetic Beads Panel
Standard Curves
1 10 100 1,000 10,000 100,000
Concentration (pg/mL)
Amylin (active)
C-Peptide
Ghrelin (active)
GIP (total)
GLP-1 (active)
Glucagon
IL-6
Insulin
Leptin
MCP-1
PP
PYY
TNFα

Comparison of Human Metabolic Hormone Panel
(Non-Magnetic Beads) and Human Metabolic
Hormone Panel (Magnetic beads)
Millipore now offers two different types of Human
Metabolic Hormone Panels for use with Luminex xMAP
technology. The kits contain identical components, except
for the magnetic beads. Our regular polystyrene bead kit
contains fluorescently dyed polymer beads with an
antibody attached to surface carboxyl groups, creating
covalent bonds on the bead surface. Magnetic polystyrene
beads contain encapsulated magnetite, and bead surface
chemistries are identical to non-magnetic beads.
The advantage of the magnetic bead is that it allows more
rapid and consistent wash steps. Millipore provides both
magnetic and non-magnetic kits so that you can decide
which format is best for your laboratory. Our studies have
shown that the two kits perform identically (see data
below), so you can be assured that your magnetic bead
assay results will be identical to results from nonmagnetic
bead kits! To order our kits or speak to a
technical service representative, call 1-866-441-8400 or
visit us online at: www.millipore.com/milliplex_ordering.
MFI
Comparison of Human Metabolic Hormone
Panels (Magnetic and Non-Magnetic Beads)
Insulin
100,000
10,000
1,000
100
10
1
10,000
1,000
MFI 100,000
100
10
1
1 10 100
pM
Magnetic
Non-Magnetic
1,000 10,000
Comparison of Human Metabolic Hormone
Panels (Magnetic and Non-Magnetic Beads)
C-Peptide
Magnetic
Non-Magnetic
ADVANTAGES OF MAGNETIC BEAD kITS
• Walk-away washing
• Lower and more consistent coefficients of variation (CVs)
• High throughput
Comparison of Human Metabolic Hormone
Panels (Magnetic and Non-Magnetic Beads)
• Improved performance with Insulin “sticky” samples
(e.g. 100,000 samples with high nonspecific binding)
10,000
• Flexible – can be washed manually with filter plates
MFI
MFI
1,000
100
10
1
10,000
1,000
100
10
1
1 10 100
pM
1 10100 pM
Magnetic
Non-Magnetic
1,000 10,000
Comparison of Human Metabolic Hormone
Panels (Magnetic and Non-Magnetic Beads)
C-Peptide
100,000
Magnetic
Non-Magnetic
1,000 10,000
HUMAN
21

22
Luminex xMAP Technology
With xMAP technology, you will benefit from
the ability to simultaneously quantitate up to
100 analytes in a single sample, using as little
as 25 µL or less.
• Speed/High-Throughput: Simultaneously measure
the concentration of a large number of analytes,
enabling you to do more faster, gaining early and
comprehensive data so critical to your work.
xMAP TECHNOLOGY PROCESS FLOW
Bead #56, TNF-α
Bead #29, IFN-γ
Bead #12, IL-6
1 Luminex uses proprietary techniques
to internally color-code microspheres
with two fluorescent dyes. Through
precise concentrations of these dyes,
100 distinctly colored bead sets can be
created, each of which is coated with a
specific capture antibody.
2 After an analyte from a test sample is
captured by the bead, a biotinylated
detection antibody is introduced. The
reaction mixture is then incubated with
Streptavidin PE conjugate, the reporter
molecule, to complete the reaction on
the surface of each microsphere.
COMPARISON OF THE xMAP & ELISA TECHNOLOGIES
• Accuracy: xMAP technology generates real-time
analysis and accurate quantification of biological
interactions.
• Reproducibility: The high-volume production of
xMAP beads allows assay standardization that
solid-phased flat arrays cannot provide.
Side-by-side comparison of Multiplexing vs. ELISAs (Study of 10 different analytes in 80 test samples).
xMAP Technology ELISA
Number of plates required 1 10
Results per plate 800 80
Total sample used per panel 50 µL 4 mL
Internal controls possible Yes No
Dynamic Range 1-10,000 pg/mL 10-2500 pg/mL
Lower limit of detection ~1 pg/mL ~1 pg/mL
3 The microspheres are allowed to
pass rapidly through a laser, which
excites the internal dyes marking the
microsphere set. A second laser excites
PE, the fluorescent dye on the reporter
molecule.

MILLIPLEX MAP: Everything You Need –
All In One Place
MILLIPLEX MAP
MILLIPLEX Analyzer
MILLIPLEX Analyst
BioMarker Services
PRODUCTS
• Higher Quality – Industry-leading QC, QA and Six Sigma Principles
• More Efficient – Analytically validated panels for consistency and reproducibility
• All-In-One Kit – A single kit with a single catalog number
• More Flexible – Select only the analytes needed or choose a pre-mixed kit
• Continuity of Knowledge Is the Key – Millipore “owns” every step of the process,
from R&D to manufacturing
• More Choices – The broadest range of multiplex analytes for the Luminex xMAP platform
INSTRUMENTS
• Speed/High-Throughput – Simultaneously measure the concentration of a large number
of different analytes, allowing you to do more faster, gaining early and comprehensive
data so critical to your work.
• Accuracy – xMAP technology generates real-time analysis and accurate quantification of
the biological interactions.
• Reproducibility – High-volume production of xMAP microspheres allows assay
standardization that solid-phased flat arrays cannot provide.
• Low Sample Volume – With minimal hands-on time, you can screen more than 40
analytes in a single sample using as little as 25 µL or less.
SOFTWARE
• The Luminex xPONENT ® software helps you manage and track all of your multiplex
assays by combining ease of use and flexibility to perform both protein and nucleic acid
testing on one system. It’s also compatible with the upcoming magnetic bead (MagPlex beads) assays, and will be moving forward with compatible future instrument platforms.
• NEW! Millipore now offers MILLIPLEX Analyst software, developed exclusively for
Millipore by VigeneTech ® . This data analysis package helps you do your best work with
increased speed and efficiency. MILLIPLEX Analyst software provides a whole new level
of data analysis by allowing you to import large data sets and benefit from the ability to
calculate your results in seconds. The user-friendly plate setup feature lets you work
with speed and efficiency while the unique algorithms on even the most challenging data
sets give you the performance you need for multiplexing analysis.
SERVICES
• Millipore’s BMS (a part of BioPharma Services) has been performing immunoassays for
over 25 years.
• Assay services are available for RIAs, ELISAs, IRMAs and multiplex assays, using both
Millipore kits as well as any commercially available assays.
• Quality control, reproducible results, and confidentiality are of paramount importance
to our BMS team.
• Millipore’s Regulatory Compliant Laboratory is uniquely positioned to provide
bioanalytical services to support your preclinical and clinical studies.
For a complete and up-to-date list of MILLIPLEX MAP analytes, visit www.millipore.com/analytes.
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ORDERING INFORMATION
Description Quantity Catalogue No.
adipocyte /
adipokine Panels
Human Serum Adipokine Panel A 96-well plate HADK1-61K-A
Human Serum Adipokine Panel B 96-well plate HADK2-61K-B
Human Adipocyte Panel 96-well plate HADCYT-61K
Mouse Serum Adipokine Panel 96-well plate MADPK-71K
Mouse Adipocyte Panel 96-well plate MADPCYT-72K
Mouse Adiponectin Single Plex 96-well plate MADPK-71K-ADPN
Rat Adipokine Panel 96-well plate RADPK-81K
Rat Adipocyte Panel 96-well plate RADPCYT-82K
Rat Adiponectin Single Plex 96-well plate RADPK-81K-ADPN
Canine Adipokine Panel 96-well plate CADPK-91K
Endocrine Panels Human Endocrine Panel 96-well plate HENDO-65K
Gut Hormone
Panels
Mouse Endocrine Panel 96-well plate MENDO-75K
Rat Endocrine Panel 96-well plate RENDO-85K
Canine Endocrine Panel 96-well plate CENDO-95K
Human IGF-1 Single Plex 96-well plate HIGF1-52K-01
Rat/Mouse IGF-1 Single Plex 96-well plate RMIGF187K
Human Gut Hormone Panel 96-well plate HGT-68K
Mouse Gut Hormone Panel 96-well plate MGT-78K
Rat Gut Hormone Panel 96-well plate RGT-88K
IGF-1 Human IGF-1 Single Plex 96-well plate HIGF1-52K
Metabolic Disease
Panels
Rat/Mouse IGF-1 Single Plex 96-well plate MRIGF187K
Human, Non-Magnetic Panel 96-well plate HMH-34K
Human, Magnetic Panel 96-well plate HMHMAG-34K
OTHER RELATED PRODUCTS
• MILLIPLEX m a p Multiplex Panels
- Cardiovascular Disease (CVD): Human, Mouse, Rat
- Apolipoprotein: Human
- Thyroid: Rat
- Pituitary: Human, Rat
- Stress Hormone: Rat
- Coming Soon! Human Neuroendocrine
(brain-derived protein) Panel.
• And, more … visit: millipore.com/bmia
GPCR
• GPCR Receptors
- GLP-1 receptor: ChemiScreen membrane preps
(Cat. No. HTS0163M)
- Glucagon receptors: ChemiScreen membrane preps
(Cat. No. HTS112M)
- Ready-to-Assay frozen cells (Cat. No. HTS112F)
• GIP receptors
- ChemiScreen membrane preps (Cat. No. HTS134M)
- Ready-to-Assay frozen cells (Cat. No. HTS134F)
• And, more … visit: www.millipore.com/gpcr
antibodies
• Anti-Adiponectin, clone RADI 06 (Cat. No. MAB3854)
• Anti-Adiponectin, clone MADI 14 (Cat. No. MAB3832)
• Anti-Adiponectin, Globular Domain Region (Cat. No. MAB3608)
• Anti-Adiponectin, clone ADI773 (Cat. No. MAB3604)
• Anti-Adiponectin (Cat. No. AB3267P)
• Anti-IGF-1 (Cat. No. MAB1120)
• Anti-IGF-1 (rabbit polyclonal (Cat. No. 07-1411))
• Anti-phospho-IR/IGF1R (Tyr1158/Tyr1162/T (Cat. No. 07-841))
• Anti-phospho-IR/IGF1R (Tyr1158 (Cat. No. 07-839))
• Anti-phospho-IRS1 (Ser307 (Cat. No. 07-247))
• Anti-Insulin Receptor (b-Subunit), clone CT-3 (Cat. No. 05-1104)
• Anti-phospho-IRS1 (Ser302 mouse / Ser307 human), clone 8.1.2
(Cat. No. 05-1086)
• Anti-IRS1, agarose conjugate (Cat. No. 16-146)
• Anti-phospho-IRS1 (Y162 (Cat. No. 09-432))
• Anti-phospho-IRS1 (Ser636/Ser639 (Cat. No. 09-430))
• Anti-phospho-IRS1 (Tyr941 (Cat. No. 07-848))
• Anti-phospho-IRS1 (Tyr1229 (Cat. No. 07-845))
• Anti-phospho-IRS1 (Tyr1179 (Cat. No. 07-844))
• And, more … visit: www.millipore.com/antibodies

VigeneTech