Ipomoea (Sweetpotato/Kumara) Post-Entry Quarantine Testing Manual

Bacterial isolation on media
Isolation of regulated bacteria testing must be carried out using the original I. batatas and I.
setosa plants and should be done in summer (or under summer-like conditions). For each
plant, at least two fully expanded leaves must be sampled from each of two different branches
of the main stem, one a younger leaf and one an older leaf from a mid-way position.
Detection of bacteria requires plating vascular tissue from petioles mid-veins. Each plant in
the glasshouse must be tested individually. See section 7.1.4 for further details.
Table 1: Summary of the regulated pests for I. batatas and I. setosa indicating the
specific tests that are required (■), alternative (□) or optional ()
Organism Type Graft
Inoculation 1
Herbaceous
Indexing
Ipomoea Post-Entry Quarantine Testing Manual · November 2012
ELISA PCR
Isolation
on media
Inspection
Mites
Tetranychus evansi ■
Bacterium
Dickeya chrysanthemi □ □
Phytoplasma
Sweetpotato little leaf
phytoplasma
Viruses
■
Sweetpotato caulimo-like virus ■
Sweetpotato chlorotic stunt
virus
■ ■
Sweetpotato leaf curl virus 2 ■ ■
Sweetpotato leaf speckling
virus
■
Sweetpotato mild speckling ■
virus
Sweetpotato vein mosaic virus ■ □ □
Sweetpotato yellow dwarf virus ■ ■
Tobacco streak virus ■ □ □
1 2
Not required for I. setosa; ssDNA Geminivirus
7.1 Specific tests for nursery stock
Each plant must be tested separately with the following exceptions, samples from up to 5
plants may be bulked for testing provided that either:
(a) the plants are derived from a single imported plant or plant established from a storage
root from which separate cuttings have been taken upon arrival in New Zealand, in
the presence of a MPI inspector; or
(b) in the case of tissue culture where plants are clonal, and this is confirmed by evidence
from the national plant protection organisation in the exporting country.
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