Ipomoea (Sweetpotato/Kumara) Post-Entry Quarantine Testing Manual
Ipomoea (Sweetpotato/Kumara) Post-Entry Quarantine Testing Manual
Ipomoea (Sweetpotato/Kumara) Post-Entry Quarantine Testing Manual
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17. Wash DNA pellets with 500 µl ice-cold 70% (v/v) ethanol, centrifuge at 4 o C or at room<br />
temperature for 10 min at 13000 rpm.<br />
18. Dry DNA pellets in the DNA concentrator or air-dry.<br />
19. Resuspend in 20 µl sterile distilled water. Incubating the tubes at 55 o C for 10 min can aid<br />
DNA resuspension.<br />
20. Store DNA at -20ºC for short-term storage or -80ºC for long-term storage.<br />
<strong>Ipomoea</strong> <strong>Post</strong>-<strong>Entry</strong> <strong>Quarantine</strong> <strong>Testing</strong> <strong>Manual</strong> · November 2012<br />
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