Ipomoea (Sweetpotato/Kumara) Post-Entry Quarantine Testing Manual

Contents
Ipomoea Post-Entry Quarantine Testing Manual
1. SCOPE ....................................................................................................................................................... 1
2. INTRODUCTION ..................................................................................................................................... 1
3. IMPORT REQUIREMENTS................................................................................................................... 3
4. PESTS ........................................................................................................................................................ 3
4.1 Regulated pests for which generic measures are required ........................................................... 3
4.2 Regulated pests for which specific tests are required ................................................................... 4
5. PROPAGATION, CARE AND MAINTENANCE IN POST-ENTRY QUARANTINE .................... 4
5.1 Whole plants..................................................................................................................................... 4
5.2 Plants in tissue culture .................................................................................................................... 5
5.3 Pollen ................................................................................................................................................ 5
6. INSPECTION ............................................................................................................................................ 5
7. TESTING ................................................................................................................................................... 6
7.1 Specific tests for nursery stock ....................................................................................................... 7
7.1.1 Graft inoculation ......................................................................................................................... 8
7.1.2 Herbaceous indexing................................................................................................................. 10
7.1.3 Serological and molecular assays ............................................................................................ 11
7.1.3.1 Enzyme-linked immunosorbent assay (ELISA) .......................................................... 11
7.1.3.2 Polymerase chain reaction (PCR)................................................................................. 12
7.1.3.2.1 Virus reverse transcription-PCR .............................................................................. 14
7.1.3.2.1.1 Sweet potato chlorotic stunt virus ........................................................................ 18
7.1.3.2.1.2 Sweetpotato leaf curl virus ................................................................................... 18
7.1.3.2.1.3 Sweetpotato mild speckling virus ......................................................................... 18
7.1.3.2.1.4 Sweetpotato vein mosaic virus .............................................................................. 18
7.1.3.2.1.5 Tobacco streak virus ............................................................................................. 18
7.1.3.2.2 Phytoplasma PCR ....................................................................................................... 19
7.1.3.2.2.2 Sweetpotato little leaf phytoplasma ................................................................... 21
7.1.3.2.3 Bacteria PCR .............................................................................................................. 21
7.1.3.2.3.1 Dickeya chrysanthemi .......................................................................................... 21
7.1.4 Bacterial isolation on media ..................................................................................................... 22
7.1.4.1 Dickeya chrysanthemi (basonym. Erwinia chrysanthemi) ........................................... 22
7.1.5 Microscopic inspection for mites ............................................................................................. 23
7.1.5.1 Tetranychus evansi ......................................................................................................... 23
8. CONTACT POINT ................................................................................................................................. 24
9. ACKNOWLEDGEMENTS .................................................................................................................... 24
10. REFERENCES ........................................................................................................................................ 24
Appendix 1. Symptoms of significant regulated pests of Ipomoea batatas .................................................. 27
1.1 Meliodogyne incognita ................................................................................................................... 27
1.2 Rotylenchulus reniformis ............................................................................................................... 27
1.3 Tetranychus evansi ......................................................................................................................... 27
1.4 Plant damage caused by mites ...................................................................................................... 27
1.5 Streptomyces ipomoea .................................................................................................................... 28
1.6 Elsinoë batatas ................................................................................................................................ 28
Ipomoea Post-Entry Quarantine Testing Manual · November 2012
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