Ipomoea (Sweetpotato/Kumara) Post-Entry Quarantine Testing Manual

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3. IMPORT REQUIREMENTS The import requirements for I. batatas and I. setosa nursery stock (whole plant and plants in tissue culture) are set out in MPI’s import health standard “Importation of Nursery Stock” (http://www.biosecurity.govt.nz/files/ihs/155-02-06.pdf). Imported nursery stock must meet the general requirements (sections 1-2) and the specific requirements detailed in the “Ipomoea batatas” schedule. On arrival in New Zealand, the nursery stock must be grown for a minimum period of 3 months in a Level 3 post-entry quarantine facility where it will be inspected, treated and/or tested for regulated pests. The import requirements for Ipomoea seed for sowing are set out in MPI’s import health standard “Importation of Seed for Sowing” (http://www.biosecurity.govt.nz/files/ihs/155-02- 05.pdf). Imported seed is only required to meet the general requirements (sections 1-2) and there are no specific requirements for the genus. An import permit is not required and seed meeting the import requirements is given biosecurity clearance at the border without the need for post-entry quarantine. The import requirements for pollen are stated in section 2.2.3 in MPI’s import health standard “Importation of Nursery Stock” (http://www.biosecurity.govt.nz/files/ihs/155-02-06.pdf ) and further details can be found in section 5.3 of this manual. 4. PESTS The following section lists regulated pests of I. batatas and I. setosa nursery stock that require generic or specific measures. 4.1 Regulated pests for which generic measures are required Insects: Cylas formicarius Cylas puncticollis Euscepes postfasciatus Nematodes: Meliodogyne incognita [Fig. 1.1] Rotylenchulus reniformis [Fig. 1.2] Pratylenchus coffeae Pratylenchus brachyurus Fungi: Elsinoë batatas [Fig. 1.6] Helicobasidium mompa Bacteria: Pseudomonas batatas Streptomyces ipomoea [Fig. 1.5] Xanthomonas batatae Xylella fastidiosa Ipomoea Post-Entry Quarantine Testing Manual · November 2012 3
Viruses: Sweetpotato chlorotic fleck virus Sweetpotato latent virus Sweetpotato ringspot virus Sweetpotato virus C6 [Fig. 1.8] Ipomoea Post-Entry Quarantine Testing Manual · November 2012 [Fig. 1.8, 2.3] 4.2 Regulated pests for which specific tests are required Mites: Tetranychus evansi [Fig. 1.3, 1.4] Bacteria: Dickeya chrysanthemi [Fig. 1.7] Phytoplasma: Sweetpotato little leaf phytoplasma [Fig. 1.11] Viruses: Sweetpotato caulimo-like virus Sweetpotato chlorotic stunt virus [Fig. 1.9, 2.1] Sweetpotato leaf curl virus [Fig. 1.10, 2.4, 2.5, 2.6] Sweetpotato leaf speckling virus Sweetpotato mild speckling virus Sweetpotato vein mosaic virus Sweetpotato yellow dwarf virus Tobacco streak virus 5. PROPAGATION, CARE AND MAINTENANCE IN POST-ENTRY QUARANTINE 5.1 Whole plants Sweetpotato plants can be grown in the glasshouse all year round as long as a day-time temperature between 18-26ºC is maintained. Night-time temperatures should not fall below 12ºC to avoid chilling injury. Supplementary lighting may be required in winter. Sweetpotatoes require free-draining planting which is initially only moistened to avoid development of rots in quiescent storage roots. The plants can be watered more freely when the canopy has established and the plants are actively growing. The sweetpotato is a perennial plant and harvesting takes place when storage roots reach the desired size. Harvesting may be plant destructive, but if larger storage roots are removed with minimal plant disturbance, the remaining storage roots will continue to grow and new plants will form. Harvested roots should be stored in the dark at 13ºC. Storage temperatures should not fall below 12ºC which can cause chilling injury. Relative humidity during storage should be maintained at 80 to 90%. Roots stored in multi-walled paper bags can respire and maintain their own humid environment. Hessian or net bags should be avoided as they can cause abrasive injury and allow moisture and pathogen entry. 4
Page 1 and 2: Ipomoea (Sweetpotato/Kumara) Post-E
Page 3 and 4: 1.7 Dickeya chrysanthemi ..........
Page 5: The per capita income provided by s
Page 9 and 10: 7. TESTING Each of the specific tes
Page 11 and 12: 7.1.1 Graft inoculation Each I. bat
Page 13 and 14: 7.1.2 Herbaceous indexing Each I. b
Page 15 and 16: Table 2: Source of antisera and pos
Page 17 and 18: 7.1.3.2.1 Virus reverse transcripti
Page 19 and 20: Recommended method for RNA viruses:
Page 21 and 22: Positive control material, in the f
Page 23 and 24: The reaction and cycling conditions
Page 25 and 26: (a) Primers recAF/recAR (Waleron et
Page 27 and 28: 8. CONTACT POINT This manual was de
Page 29 and 30: Nassar, A; Darrasse, A; Lemattre, M
Page 31 and 32: . 1.5 Streptomyces ipomoea (a) (b)
Page 33 and 34: Appendix 2. Virus symptoms on graft
Page 35 and 36: Appendix 3. Protocols referenced in

Ipomoea (Sweetpotato/Kumara) Post-Entry Quarantine Testing Manual

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