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Jungermanniales MUM Fossombronia Haplomitrium Lophocolea Sphagnales Sphagnum Buzbaumiales Burbaumia Dicranales Ceratodon Pottiales Phascum Funariales Aplodon Funaria Physcomitrella Splachnum Schistostegales Schistostega Tetraphidales Tetraphis Eubryales Aulacomnium Bryum Leptobryum Hypnobryales Hypnum (Amblystegium) ANGIOSPERMAE ARALES Lemnaceae Wolffia 4.6
LIST OF CULTURE MEDIA Details of some of the more useful maintenance media, referred to by number in the list of strains, are given below. Special media and methods for algae are given by Venkataraman (1969) and Stein (1973); for parasitic protozoa by Taylor and Baker (1978); for free living protozoa by Page (1981), and for various microorganisms in a CRC Handbook by Rechcigl (1978). The water used should always be distilled or deionised (unless the contrary is stated). Sterilization is by autoclaving at 15 lb/in2 (ca 1 bar or 101 kPa) for 15 min unless stated other— wise. Mention of a particular supplier does not imply that other products are necessarily less satisfactory. Medium M1 This medium is satisfactory for many algae; it quickly reveals the presence of most contaminants. For agar cultures with bacteria, a less rich medium is necessary such as soil extract agar (e + s). Medium M2 (e + s) KNO3 K2HPO4 MgSO4.7H20 Agar Soil extract stock solution g per 100 ml water 0.02 0.002 0.002 1.0 10% by volume The soil extract stock solution is made by heating in a steamer a calcareous garden loam with twice its volume of supernatant water for 2 hours, or by autoclaving for 15 min. It is convenient to make up and sterilize a number of small containers of stock solution each of a size appropriate to making a batch of media, as repeated autoclaving is deleterious. 5.1 SOIL AND WATER MEDIA These simple media have great advantages for many purposes, so long as axenic culture is not required, and may produce excellent growth of almost any organism apart from the more exacting planktonic and parasitic forms. Medium M3 Basic biphasic medium Put a layer about 1 cm deep of good calcareous garden loam into a test tube or jar. (The use of mud from rivers or ponds is seldom satisfactory.) Carefully add water to a depth of 7 to 10 cm, plug or cover, and steam for one hour (longer for larger vessels) on each of 2 consecutive days; further sterilization is not needed. Allow to stand for a further day before inoculating, when the pH should be between 7 and 8. Many variations of this basic medium are possible. The garden soil can be replaced by calcareous clay (Medium M3i). The addition of a little (about 3% of the volume of the soil) calcium carbonate or ammonium magnesium phosphate beneath the soil is recommended, the former (Medium M3ii) for many eutrophic Chlorophyceae, the latter (Medium M3iii) for many green euglenids. Sphagnum peat may be added or may replace the soil when growing forms from acid habitats (Medium M3iv). The addition of a little starch below the soil stimulates growth of many saprophytes like Polytoma and Astasia (Medium M3v). A grain of pearl barley, rice or wheat produces a bacterial flora forming suitable food for many ciliates (Medium M3vi). When selecting soils, it is advisable to take a fair—sized sample, about one cubic foot or 30 litres, pass this through a sieve of about 1 cm mesh and then make up media and test them with appropriate organisms. If the sample is satisfactory, sufficient stock is then available for many batches of medium.
Page 1: . ' stLx ;AL: le f It A
Page 4 and 5: NERC Copyright 1982 Printed in Grea
Page 7 and 8: INTRODUCTION The Culture Centre of
Page 9: LIST OF OTHER CULTURE COLLECTIONS F
Page 12 and 13: DINOPHYCEAE Amphidinium (marine) Cl
Page 15 and 16: LIST OF GENERA IN THE COLLECTION 4.
Page 17 and 18: Rhizochromulina Spume Ua Syncr-ypta
Page 19: SARCODINA Schizoplasmodium Cymnamoe
Page 23 and 24: Medium M9 Potytoma medium (Polyt.)
Page 25 and 26: Medium 1118 Cerophyl—Prescott liq
Page 27 and 28: LIST OF ALGAE. PROTOZOA AND ROTIFER
Page 29 and 30: CCAP 1463/13c CCAP 1403/13d CCAP 14
Page 31 and 32: Ankistrodesmus CCAP 202/13 Ankistro
Page 33 and 34: ASTEROCYTIS Gobi Audouinella newton
Page 35 and 36: BRACHIOMONAS Bohlin Bumileria sicul
Page 37 and 38: CHARACIOSIPHON lyengar Chlamydomona
Page 39 and 40: Chlamydomonas CCAP 11/63 Chlamydomo
Page 41 and 42: CHLORELLA Beijerinck It has long be
Page 43 and 44: CCAP 211/11p CCAP 211111q CCAP 211/
Page 45 and 46: Chloromonas sp. indet. CCAP 11/50 C
Page 47 and 48: Chrysochromulina kappa Parke a Mant
Page 49 and 50: Coccomyza subellipsoidea Acton CCAP
Page 51 and 52: CRUCICENIA Morren Cryptomonas calce
Page 53 and 54: 627 CCAP 1415/1 Fogg; 1945; LB; fre
Page 55 and 56: Duna Henn minuta Lerche Dunaliella
Page 57 and 58: Euglena caudata Hubner CCAP 1224/24
Page 59 and 60: Euglena tristella Chu CCAP 1224134
Page 61 and 62: FREMYELLA J. de Toni Fremyella dipl
Page 63 and 64: HALOSPHAERA Schmitz Halosphaera rus
Page 65 and 66: Hymenomonas pringsheimii Parke a Gr
Page 67 and 68: MENOIDIUM Perty Menoidium bibacilla
Page 69 and 70: Myrmecia reticulata Tschermak-Woess
Page 71 and 72:
CCAP 252/1 Belcher; 1961; N; Englan
Page 73 and 74:
CCAP 933/13d Butcher; LB; England;
Page 75 and 76:
Paramecium primaurelia Sonneborn CC
Page 77 and 78:
CCAP 1261/2c Pringsheim; 1940; LB;
Page 79 and 80:
PLEODORINA Shaw Pleodorina illinois
Page 81 and 82:
PRO7'OSTELIUM Olive a Stoianovitch
Page 83 and 84:
Pseudotrebouxia decolorans (Ahmadji
Page 85 and 86:
RHODELLA Evans Scenedesrnus acutifo
Page 87 and 88:
6.61 SELENASTRUM Reinsch SPHAEROCYS
Page 89 and 90:
STAURAS7'RUM Ralfs Staurastrum chae
Page 91 and 92:
SYNCRYPTA Ehr. Syncrypta glomerifer
Page 93 and 94:
Tetrahym.ena rostrata (Kahl) CCAP 1
Page 95 and 96:
Thecamoeba terricola (Greeff) Trach
Page 97 and 98:
CCAP 1981/4 Trypanosoma hedricki Bo
Page 99 and 100:
Vischeria punctata Vischer Vorticel
Page 101 and 102:
LIST OF BRYOPHYTES AND HIGHER PLANT
Page 103 and 104:
REFERENCES Bischoff, H.W. s Bold, H
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