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Medium M9 Potytoma medium (Polyt.) (ii) Extra salts
Medium MIO PPG medium for axenic Acanthamoeba
Amoeba saline: Five stock solutions are prepared as
Make final medium by adding 10 ml of each stock
solution to 950 ml of.glass distilled water.
MARINE ALGAE
The standard medium for marine algae used at CCAP is:
Medium Mil (M)
See also Pennick & Cann (in press)
Stock solutions
(i) Major salts
Sea Salts -
Tris(hydroxymethyl)amino-
methane (NH2.C.(CH2OH)3) 0.5 g
- Supplied by Water Life Research Industries.
Quantities per'litre
5.3
(iii) Vitamins
Biotin 0.2 mg
Calcium pantothenate 20.0 mg
Cyanocobalamin 4.0 mg
Folic acid 0.4 mg
Inositol 1000.0 mg
Nicotinic acid 20.0 mg
Thiamine 100.0 mg
Thymine 600.0 mg
(Vitamin solution may be stored frozen at -20°C)
Preparation: 50 ml of soil extract is added to
950 ml of stock solution (i) and 3.75 ml of stock
solution (ii), then the pH is adjusted to 7.6 - 7.8
using 1N HC1. It is then filtered, dispensed into
appropriate containers and autoclaved. Vitamin
solution (iii) is added after sterilization through
a 0.22 micrometre filter, 0.25% by volume.
Many marine algae grow in Erdschreiber medium:
Medium M12
Natural seawater (or artificial
sea salts, see Medium M11) 1 litre
Soil extract stock solution
(see Medium M2)
NaNO3
Na2HPO4.12H20
33.6 g in this medium.
50.0 ml
0.2 g
0.03 g
'Natura' or 'Synthetica' Some marine ciliates and amoebae are also grown