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Medium M4 Bold's basal medium (Bischciff a Bold, 1963) HC1 (1N) 0.25 ml
This is very useful for many algae including those EDTA.Na2 2.0 mg dissolved)
from more or less eutrophic waters or from soils. It together)
may be supplemented by soil extract (see MediuM M2) FeC13 1.0 mg in 1 litre)'
and/or vitamins (see Medium M11).
Six stock solutions (i - vi) are used, each
H3B03 2.48 mg
containing one of the following salts dissolved MnC12.4H20 1.39 mg
in 400 ml distilled water:
NaNO3 10.0 g
(ii) CaC12.2H20 1.0 g
(iii) MgSO4.7H20 3.0 g
(iv) K2HPO4 3.0 g
(NH4)8Mo2024.4H20 1.00 rng
vitamin B12 0.01 mg
vitamin B1 0.001 mg
Biotin 0.001 mg
(v) KH2PO4 7.0 g Many strains require more specialized media; some of
(vi) NaCI 1.0 g
Four trace-element solutions (vii-x) are prepared
as follows, all dissolved in 1 litre of distilled
water:
(vii) EDTA
KOH
50 g
31 g
(viii) FeSO4.7H20 4.98 g
(use acidified H20 : 1.0 ml of H2SO4
added to 999 ml distilled water)
(ix) H3B03
(x) ZnSO4.7H20
MnC12.4H20
Mo03
CuSO4.5H20
Co(NO3)2
11.42 g
8.82 g
1.44 g
0.71 g
1.57 g
0.49 g
10 ml of each stock solution (i) - (vi) and 1.0 ml
each of solutions (vii) - (x) are added to 940 ml
distilled water.
Medium M5 Modified Chu 10 (EVT) (Chu, 1942)
Amounts per litre
52
the most important are given below:
Medium M6 Euglena gracilis medium (E.g.)
Sodium acetate (hydrated) 0.1
Beef extract 0.1
Yeast extraCt 0.2
Bacto tryptone 0.2
g per 100 ml
Ca Cl2 0.001
Agar (optional) 1.0
Medium M7 Ochromonas medium (Ochr)
(for axenic freshwater Ochromonas spp. only)
Liver infusion (dehydrated) Oxoid 0.1
Glucose 0.1
Bacto tryptone 0.1
Ca(NO3)2.4H20 20.0 mg Medium M8 Tetrahymena m.edium (P.Y.)
g per 100 ml
KH2PO4 6.2 mg g per 100 ml
MgSO4.7H20 25.0 mg Proteose peptone 1.0 or 2.0
Na2CO3 20.0 mg Yeast extract 0.25
Na2SiO3 25.0 mg