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Immobilon-FL Membrane Improves Fluorescence ... - Millipore

Immobilon-FL Membrane Improves Fluorescence ... - Millipore

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ELECTROPHORESIS & PROTEIN TRANSFER<br />

142<br />

<strong>Immobilon</strong>-P SQ <strong>Membrane</strong> Prevents<br />

Small Protein “Blow-Through”<br />

Molecular weight standards (lanes 1, 3) and calf liver<br />

lysates (lanes 2, 4) were transferred to <strong>Immobilon</strong>-P<br />

(0.45 µm) and <strong>Immobilon</strong>-P SQ membrane (0.2 µm).<br />

A sheet of <strong>Immobilon</strong>-P SQ membrane placed behind the<br />

primary membranes captured the small (


Protein Transfer Product Listing<br />

Ordering Information<br />

Type Size Qty/Pk Catalogue No.<br />

Transfer <strong>Membrane</strong>s<br />

<strong>Immobilon</strong>-P Transfer <strong>Membrane</strong> (0.45 µm)<br />

Cut Sheet 7 x 8.4 cm 50 IPVH 078 50<br />

8 x 10 cm 10 IPVH 081 00<br />

8.5 x 13.5 cm 10 IPVH 081 30<br />

9 x 12 cm 10 IPVH 091 20<br />

10 x 10 cm 10 IPVH 101 00<br />

15 x 15 cm 10 IPVH 151 50<br />

20 x 20 cm 10 IPVH 202 00<br />

26 x 26 cm 10 IPVH 304 F0<br />

Roll 26.5 x 375 cm 1 IPVH 000 10<br />

<strong>Immobilon</strong>-P SQ Transfer <strong>Membrane</strong> (0.2 µm)<br />

Cut Sheet 7 x 8.4 cm 10 ISEQ 078 50<br />

8 x 10 cm 10 ISEQ 081 00<br />

8.5 x 13.5 cm 10 ISEQ 081 30<br />

9 x 12 cm 10 ISEQ 091 20<br />

10 x 10 cm 10 ISEQ 101 00<br />

15 x 15 cm 10 ISEQ 151 50<br />

20 x 20 cm 10 ISEQ 202 00<br />

26 x 26 cm 10 ISEQ 262 60<br />

Roll 26.5 x 375 cm 1 ISEQ 000 10<br />

<strong>Immobilon</strong>-<strong>FL</strong> Transfer <strong>Membrane</strong> (0.45 µm)<br />

Sheet 10 x 10 cm 10 IP<strong>FL</strong> 101 00<br />

20 x 20 cm 10 IP<strong>FL</strong> 202 00<br />

Roll 26.5 cm x 3.75 m 1 IP<strong>FL</strong> 000 10<br />

Blotting Sandwiches<br />

<strong>Immobilon</strong>-P membrane interleaved 7 x 8.4 cm 20 IPSN 078 52<br />

with blotting paper 5 cm 8.5 x 13 cm 20 IPSN 081 32<br />

Blotting Filter Paper<br />

Pre-cut sheets of filter paper 7 x 8.4 cm 100 IBFP 078 5C<br />

8.5 x 13.5 cm 100 IBFP 081 3C<br />

Description Volume Qty/Pk Catalogue No.<br />

Western Blot Detection Substrates<br />

<strong>Immobilon</strong> Western Chemilimunescent HRP Substrate 20 mL 20 mL 1 WBKLS0020<br />

<strong>Immobilon</strong> Western Chemilimunescent HRP Substrate 50 mL 50 mL 1 WBKLS0050<br />

<strong>Immobilon</strong> Western Chemilimunescent HRP Substrate 100 mL 100 mL 1 WBKLS0100<br />

<strong>Immobilon</strong> Western Chemilimunescent HRP Substrate 500 mL 500 mL 1 WBKLS0500<br />

<strong>Immobilon</strong> Western Chemilimunescent AP Substrate 25 mL 25 mL 1 WBKDS0025<br />

<strong>Immobilon</strong> Western Chemilimunescent AP Substrate 100 mL 100 mL 1 WBKDS0100<br />

Visualizer Spray & Glow ECL Western Blotting Detection System 100 mL 50 mini-gel-sized<br />

blots for 1000 cm<br />

17-373<br />

2<br />

of membrane<br />

Visualizer Spray & Glow ECL Western Blotting Detection System 40 mL 20 mini-gel-sized<br />

blots for 1000 cm<br />

17-373SP<br />

2<br />

of membrane<br />

Visualizer Spray & Glow ECL Western Blotting Detection System 300 mL 1 17-373BP<br />

ELECTROHORESIS AND PROTIEN TRANSFER ORDERING INFORMATION<br />

Visit www.millipore.com for the most up-to-date product listing 143


ELECTROPHORESIS & PROTEIN TRANSFER<br />

ORDERING INFORMATION<br />

144<br />

Description Volume Qty/Pk Catalogue No.<br />

Western Blot Reagents<br />

Markers<br />

ChemiBlot Molecular Weight Markers 40 µL 5 vials/pk 2230<br />

ChemiBlot Molecular Weight Markers, trial 40 µL 1 2230-S<br />

Phosphotyrosine Molecular Weight Standards 150 blots 12-256<br />

Autorad Orientation Markers 5 markers/pk 20-135<br />

Reversible Stain<br />

BLOT - FastStain 1 kit 2076<br />

Stripping Solutions<br />

ReBlot Plus Kit 1 kit 2500<br />

ReBlot Plus Kit - Trial 1 kit 2500-S<br />

ReBlot Plus Mild Antibody Stripping Solution, 10x 50 mL 1 2502<br />

ReBlot Plus Strong Antibody Stripping Solution, 10x 50 mL 1 2504<br />

ReBlot Western Blot Recycling Kit 1 kit 2060<br />

ReBlot Western Blot Recycling Kit, trial 1 kit 2060-S<br />

Description Applications Qty/Pk Catalogue No.<br />

Pre-made Blots<br />

Endothelial Cell Blot, Normal Human WB 1 blot CB310<br />

Rat Multiple - Brain, Kidney, Liver, Heart, Lung, Pancreas, Spleen, and Testis WB 1 blot TB320<br />

Ready-to-Screen Tissue BLOTS Human Heart WB 1 blot TB032<br />

Ready-to-Screen Tissue BLOTS Human Multiple - Brain, Heart, Liver, Lung, WB 1 blot TB300<br />

Kidney, Placenta, Pancreas, Ovary, and Testis<br />

Ready-to-Screen Tissue BLOTS Mouse Multiple Tissue Blot- Brain, Heart, WB 1 blot TB310<br />

Liver, Lung, Kidney, Spleen, Testis, and Ovary

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