B12 METABOLISM IN HUMANS By NICOLE AURORA LEAL A ...
B12 METABOLISM IN HUMANS By NICOLE AURORA LEAL A ...
B12 METABOLISM IN HUMANS By NICOLE AURORA LEAL A ...
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Absorbance<br />
0.5<br />
0.4<br />
0.3<br />
0.2<br />
0.1<br />
0.0<br />
400<br />
87<br />
440 480 520 560 600<br />
Wavelength, (nm)<br />
Figure 3-4. Absorbance spectra of an MSR-ATR assay. Prior to the addition of NADPH<br />
the spectrum is that of cob(I)alamin (············); 1 hour after the addition of<br />
NADPH and incubation at 37°C, the spectrum is that of AdoCbl (——); after<br />
photolysis, the spectrum is characteristic of cob(II)alamin (⎯ ⎯ ⎯).