B12 METABOLISM IN HUMANS By NICOLE AURORA LEAL A ...

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130 Guest, J. R., E. L. Smith, D. D. Woods and S. Friedman. 1962b. A methyl analogue of cobamide coenzyme in relation to methionine synthesis by bacteria. Nature 195:340-342. Hall, D. A., T. C. Jordan-Starck, R. O. Loo, M. L. Ludwig and R. G. Matthews. 2000. Interaction of flavodoxin with cobalamin-dependent methionine synthase. Biochemistry 39:10711-9. Halpern, J. 1985. Mechanisms of coenzyme B12-dependent rearrangements. Science 227:869-75. Harder, J., R. Eliasson, E. Pontis, M. D. Ballinger and P. Reichard. 1992. Activation of the anaerobic ribonucleotide reductase from Escherichia coli by Sadenosylmethionine. J. Biol. Chem. 267:25548-52. Harlow, E. and D. Lane. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. Havemann, G. D. and T. A. Bobik. 2003. Protein content of polyhedral organelles involved in coenzyme B12-dependent degradation of 1,2-propanediol in Salmonella enterica serovar Typhimurium LT2. J. Bacteriol. 185:5086-95. Havemann, G. D., E. M. Sampson and T. A. Bobik. 2002. PduA is a shell protein of polyhedral organelles involved in coenzyme B12-dependent degradation of 1,2propanediol in Salmonella enterica serovar Typhimurium LT2. J. Bacteriol. 184:1253-61. Heithoff, D. M., C. P. Conner, U. Hentschel, F. Govantes, P. C. Hanna and M. J. Mahan. 1999. Coordinate intracellular expression of Salmonella genes induced during infection. J. Bacteriol. 181:799-807. Hodgkin, D. C., J. Pickworth, J. H. Robertson, K. N. Trueblood, R. J. Prosen and J. G. White. 1955. Crystal structure of the hexacarboxylic acid derived from B12 and the molecular structure of the vitamin. Nature 176:325-328. Horswill, A. R. and J. C. Escalante-Semerena. 1997. Propionate catabolism in Salmonella typhimurium LT2: two divergently transcribed units comprise the prp locus at 8.5 centisomes, prpR encodes a member of the sigma-54 family of activators, and the prpBCDE genes constitute an operon. J. Bacteriol. 179:928-40. Huennekens, F. M., K. S. Vitols, K. Fujii and D. W. Jacobsen. 1982. Biosynthesis of the cobalamin coenzymes, p. 145-164. In D. Dolphin (ed.), B12, vol. volume 1. John Wiley & Sons, Inc., New York. Jarrett, J. T., D. M. Hoover, M. L. Ludwig and R. G. Matthews. 1998. The mechanism of adenosylmethionine-dependent activation of methionine synthase: a rapid kinetic analysis of intermediates in reductive methylation of cob(II)alamin enzyme. Biochemistry 37:12649-58.
131 Johnson, C. L. V. J., E. Pechonick, S. D. Park, G. D. Havemann, N. A. Leal and T. A. Bobik. 2001. Functional genomic, biochemical, and genetic characterization of the Salmonella pduO gene, an ATP:cob(I)alamin adenosyltransferase gene. J. Bacteriol. 183:1577-1584. Jordan, A., E. Pontis, M. Atta, M. Krook, I. Gibert, J. Barbe and P. Reichard. 1994. A second class I ribonucleotide reductase in Enterobacteriaceae: characterization of the Salmonella typhimurium enzyme. Proc. Natl. Acad. Sci. U S A 91:12892-6. Kapadia, C. R. 1995. Vitamin B12 in health and disease: part I--inherited disorders of function, absorption, and transport. Gastroenterologist 3:329-44. Kass, L. 1978. William B. Castle and intrinsic factor. Ann. Intern. Med. 89:983-91. Kessler, D., I. Leibrecht and J. Knappe. 1991. Pyruvate formate-lyase-deactivase and acetyl-CoA reductase activities of Escherichia coli reside on a polymeric protein particle encoded by adhE. FEBS Lett. 281:59-63. Kofoid, E., C. Rappleye, I. Stojiljkovic and J. R. Roth. 1999. The 17-gene ethanolamine (eut) operon of Salmonella typhimurium encodes five homologues of carboxysome shell proteins. J. Bacteriol. 181:5317-5329. Kolhouse, J. F. and R. H. Allen. 1977. Recognition of two intracellular cobalamin binding proteins and their identification as methylmalonyl-CoA mutase and methionine synthetase. Proc. Natl. Acad. Sci. U S A 74:921-5. Korotkova, N., L. Chistoserdova, V. Kuksa and M. E. Lidstrom. 2002. Glyoxylate regeneration pathway in the methylotroph Methylobacterium extorquens AM1. J. Bacteriol. 184:1750-8. Korotkova, N. and M. E. Lidstrom. 2004. MeaB is a component of the methylmalonyl- CoA mutase complex required for protection of the enzyme from inactivation. J. Biol. Chem. 279:13652-8. Leal, N. A., S. D. Park, P. E. Kima and T. A. Bobik. 2003. Identification of the human and bovine ATP:Cob(I)alamin adenosyltransferase cDNAs based on complementation of a bacterial mutant. J. Biol. Chem. 278:9227-34. Leclerc, D., E. Campeau, P. Goyette, C. E. Adjalla, B. Christensen, M. Ross, P. Eydoux, D. S. Rosenblatt, R. Rozen and R. A. Gravel. 1996. Human methionine synthase: cDNA cloning and identification of mutations in patients of the cblG complementation group of folate/cobalamin disorders. Hum. Mol. Genet. 5:1867- 74. Leclerc, D., M. Odievre, Q. Wu, A. Wilson, J. J. Huizenga, R. Rozen, S. W. Scherer and R. A. Gravel. 1999. Molecular cloning, expression, and physical mapping of the human methionine synthase reductase gene. Gene 240:75-88.
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B12 METABOLISM IN HUMANS By NICOLE
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The work in this dissertation is de
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TABLE OF CONTENTS Page ACKNOWLEDGME
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General Molecular and Protein Metho
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LIST OF TABLES Table page 1-1. Aden
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3-7. Stoichiometry of the MSR-ATR s
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DTT dithiothreitol E. coli Escheric
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Abstract of Dissertation Presented
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CHAPTER 1 INTRODUCTION History of C
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3 (DMB). The DMB moiety is covalent
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5 and deoxyadenosine. After hydroge
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7 homocysteine recycling and methio
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methylmalonic acid. Methylmalonic a
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folate-dependent reactions includin
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tetanomorphum, P. shermanii, Euglen
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enzyme. This suggests that cob(II)a
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at the 5’-C of ATP by cob(I)alami
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iochemical studies and complementat
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1997). Both cases of the cblD disor
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23 The cblB complementation group i
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(Watkins et al. 2002). Some patient
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Chapter 2 of this dissertation repo
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Corrin Ring Dimethyl benzimidazole
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Classes Eliminases Dehydratases OH
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A) B) CH 3THF 33 MS-cob(I)alamin Me
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propanol dehydrogenase (PduQ) propa
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or from mutations that impair the a
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1989). 5-bromo-4-chloro-3-indolyl-
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41 5’-GCCGCCGGTACCGATGACGACGACAAG
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43 Growth of Adenosyltransferase Ex
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anti-rabbit IgG (γ-chain specific)
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sequence similarity to a known ATR
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ATRs were produced as fusion protei
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51 of the lacI repressor (not shown
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in which proteins bind B12, the "ba
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libraries may be particularly usefu
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57 Figure 2-1. Multiple sequence al
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Table 2-2. Specific activities of b
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61 1 2 3 4 5 25 kDa Figure 2-4. Wes
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63 (cyanocobalamin, CNCbl) and hydr
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65 5’- triphosphate (ATP), and di
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extracts of ATR 239K (160 mg protei
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Milford, MA). Samples (200 µl) wer
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Linearity of the ATR reaction 71 Th
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73 reactions was characteristic of
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75 MSR Produces little Cob(I)alamin
Page 93 and 94:
Johnson et al. 2001, Saridakis et a
Page 95 and 96: AdoCbl by the MSR-ATR system. Exper
Page 97 and 98: propionyl-CoA PCC (2S)-methylmalony
Page 99 and 100: kDa 97 66 45 31 21 14 7 83 1 2 3 4
Page 101 and 102: 85 Table 3-3. The MSR-ATR system se
Page 103 and 104: Absorbance 0.5 0.4 0.3 0.2 0.1 0.0
Page 105 and 106: Wavelength, (525 nm) 0.24 0.23 0.22
Page 107 and 108: Activity, (nmol min-1 Activity, (nm
Page 109 and 110: al. 1988). The aldehyde is then dis
Page 111 and 112: CoA-acylating propionaldehyde dehyd
Page 113 and 114: 97 previously published DNA sequenc
Page 115 and 116: mM CHES (2-[N-cyclohexylamino]ethan
Page 117 and 118: 101 For the conjugation step, strai
Page 119 and 120: 103 from New England Biolabs (Bever
Page 121 and 122: 105 Propionaldehyde Dehydrogenase A
Page 123 and 124: 107 fraction. Following centrifugat
Page 125 and 126: 109 antiserum obtained was specific
Page 127 and 128: 111 biochemical evidence was presen
Page 129 and 130: 113 Table 4-1. Bacterial strains Sp
Page 131 and 132: 115 Table 4-3. Propionaldehyde dehy
Page 133 and 134: kDa 209 80 49 35 29 117 1 2 3 4 Fig
Page 135 and 136: CHAPTER 5 CONCLUSIONS In humans, co
Page 137 and 138: 121 substitutions that are common p
Page 139 and 140: 123 Studies also indicated that hum
Page 141 and 142: LIST OF REFERENCES Aberg, A., S. Ha
Page 143 and 144: 127 Bradford, M. 1976. A rapid and
Page 145: 129 Fenton, W. A. and L. E. Rosenbe
Page 149 and 150: 133 Mellman, I., H. F. Willard and
Page 151 and 152: 135 Rossi, M., J. P. Glusker, L. Ra
Page 153 and 154: 137 Vlasie, M., S. Chowdhury and R.
Page 155 and 156: BIOGRAPHICAL SKETCH Nicole Aurora L
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