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Rocket: a Mediterranean crop for the world - Bioversity International

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transplanted plants. In 1995 both trials were planted on 5 May, while in 1996<br />

transplanting took place on 22 April and sowing on 29 April.<br />

Plant density trials were based on three plant populations (33, 50 and 100<br />

plants/m 2 ) grown in single rows 10 cm apart and with 10, 20 and 30 cm within-row<br />

spacing, fertilized as side-dress with 100 kg/ha of N.<br />

Fertilization studies were carried out with 100 plants/m and were based on<br />

three rates of nitrogen (0, 100 and 200 kg/ha). During <strong>the</strong> first year, urea was <strong>the</strong> N<br />

source, while in 1996 N was provided as ammonium nitrate and ammonium<br />

sulphate. Nitrogen was applied as side-dress on two occasions each year – 15 May<br />

and 6 June 1995, and 6 May and 5 June 1996 – <strong>for</strong> <strong>the</strong> transplanted and seeded<br />

fields, respectively.<br />

All experiments received a broadcast of preplant soil-incorporated application of<br />

100 kg/ha of P O . The experimental design was always a randomized block using<br />

2 5<br />

four replications in 1995 and three replications in 1996.<br />

Irrigation was provided as needed by overhead sprinklers. During 1995 and<br />

1996 trials <strong>the</strong> total water volume supplied was about 2000 and 2500 m 3<br />

/ha,<br />

respectively.<br />

Harvest was made by hand with knives. There were four and two cuttings in<br />

1995 and 1996, respectively. Plant material from each harvest was dried in a <strong>for</strong>cedair<br />

oven at 65°C <strong>for</strong> 48 hours to determine dry weight, fibre and nitrate content.<br />

7EPMRI [EXIV IJJIGX<br />

The experiment on salinity level and seed germination was carried out in 1995 in a<br />

controlled environment (25°C germinator, 90% relative humidity, 16/8 hours<br />

day/night photoperiod).<br />

Germination of seeds was done in embedded substrates with different salinity<br />

levels of water solution. Solutions were obtained with commercial salt (NaCl) and<br />

tapwater. Eleven treatments with ECw corresponding to 0.5, 2, 4, 6, 8, 10, 12, 14, 16,<br />

18 and 20 dS/m were made.<br />

Seeds, which had been kept in aluminium sealed envelopes at room<br />

temperature, were placed in Petri dishes, sealed with parafilm and covered with<br />

Whatman filter paper imbibed <strong>for</strong> 5 minutes with <strong>the</strong> different saline solutions.<br />

'Beginning germination' was recorded as soon as seedlings had evident<br />

cotyledons (generally <strong>the</strong> 4th day after <strong>the</strong>ir inhibition). Germination was<br />

monitored every 2 days <strong>for</strong> 2 weeks and maximum germination percentage,<br />

average time of germination (ATG, days), germination precocity (days) – 10 (T 10 ), 25<br />

(T 25 ), 50 (T 50 ), 75(T 75 ) and 90 % (T 90 ) – of total germinated seeds with normal<br />

seedling was calculated.<br />

An experiment on <strong>the</strong> influence of salinity levels on some morphologic<br />

characteristics and yield of wild rocket was conducted during 1995-96 in pots at <strong>the</strong><br />

greenhouses of <strong>the</strong> Agricultural Faculty of <strong>the</strong> University of Bari.<br />

Wild rocket seedlings were transplanted, one in each pot, with a randomized<br />

block design replicated four times and six treatments were carried out: ECw equal<br />

to 0.5 (T 1 ), 4 (T 2 ), 8 (T 3 ), 12 (T 4 ), 16 (T 5 ) and 20 dS/m (T 6 ). Transplanting took place<br />

at <strong>the</strong> beginning of December.<br />

Tapwater was used to irrigate <strong>the</strong> pots up to <strong>the</strong> end of February and later on<br />

water with <strong>the</strong> different salinity levels was used, obtained by adding commercial<br />

salt to tapwater. Watering volume was higher to meet <strong>the</strong> leaching requirement.<br />

Five harvests were made between <strong>the</strong> end of March and <strong>the</strong> beginning of July.

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