Lecture 7 - Genome Tools

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Fly genetics to fly molecular biology •WT •Dominant mutation (ey) •Suppressor mutation •Nature of… genetics v mol biol v biochem
Worm cell biology to molecular biology •RNAi can silence genes •Transgenic worm (right) with GFP reporter in head neurons •Expresses dsRNA to muscle gene unc-15 •Degrades mRNA •Causes complete paralysis •Nature of… genetics v mol biol v biochem
Page 1: (Chapt 9: Molecular genetic techniq
Page 6 and 7: Bacterial genetics: original expts
Page 8 and 9: into minimal medium---> PREVIEW: Mo
Page 10 and 11: Molecular basis- metabolic pathways
Page 12 and 13: Chemical basis- metabolic pathways
Page 14 and 15: •Epigenetic •Environmental •E
Page 16 and 17: Molecular basis- developmental path
Page 21 and 22: Genomics/bioinformatics…. complex
Page 23 and 24: Leads to....... (‘The good, the b
Page 25 and 26: Genome and manipulation of genome d
Page 27 and 28: [visually] Flower development fn of
Page 29 and 30: ‘Baby’ genetics •terminology
Page 31: ‘Baby’ cell biology: sexual rep
Page 35 and 36: Forces that cause populations and s
Page 37 and 38: Segregation of alleles in yeast
Page 39 and 40: Tools for genetics: conditional mut
Page 41 and 42: Complementation: temperature sensit
Page 43 and 44: Recombinant DNA and molecular biolo
Page 45 and 46: Host restriction (and modification)
Page 47 and 48: Host modification
Page 49 and 50: •Lambda repressor •EcoRV •htt
Page 51 and 52: Construction of ‘ideal’ vectors
Page 53 and 54:
RE, use 2: molecular cloning
Page 55:
Variations: cloning into eukaryotic
Page 59 and 60:
Plasmid (Recombinant DNA) Phage Con
Page 61:
Need for bigger inserts: Lambda pha
Page 67 and 68:
cDNA libraries in plasmids •cDNA
Page 69 and 70:
Hybridization to inserts within lam
Page 71 and 72:
Variations on Southern blotting •
Page 73 and 74:
Gel electrophoresis, blot and probe
Page 75 and 76:
End-labeling- using biochemistry of
Page 77 and 78:
•http://www.ncbi.nlm.nih.gov/book
Page 79 and 80:
Zoo blots: Comparative genomics, pr
Page 81:
•Synthesis of poliovirus from oli
Page 84 and 85:
Analysis of DNA-binding protein Ide
Page 86 and 87:
Over-expression in E. coli Western
Page 88 and 89:
Over-expression in S. cerevisiae Ga
Page 90 and 91:
Complementation: metabolic intermed
Page 92 and 93:
Use of double mutants to order step
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Lecture 7 - Genome Tools

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