Lecture 7 - Genome Tools

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Function, structure and location of gene products •Nature of… genetics v mol biol v biochem
Page 1 and 2: (Chapt 9: Molecular genetic techniq
Page 3: Worm cell biology to molecular biol
Page 7 and 8: Bacterial genetics: original expts
Page 9 and 10: Molecular basis- metabolic pathways
Page 11 and 12: Genetic and molecular basis- metabo
Page 13 and 14: Genetic and molecular basis- metabo
Page 15 and 16: •Very different heart structures
Page 17: Molecular basis- developmental path
Page 22 and 23: From Genome and manipulation of gen
Page 24 and 25: Leads to.......
Page 26 and 27: •Activation of Toll by Spatzel le
Page 28 and 29: Complex, simple- same basis -It’s
Page 30 and 31: ‘Baby’ cell biology: asexual re
Page 34 and 35: •Br/bl x bl/bl •F1: Br allele l
Page 36 and 37: What is a mutation? Molecular level
Page 38 and 39: Identification of gene, gene produc
Page 40 and 41: •cdc28 arrest pre-budding •cdc7
Page 42 and 43: Interacting or redundant proteins,
Page 44 and 45: Molecular biology: recombinant DNA
Page 46 and 47: At the molecular level
Page 48 and 49: Palindromes: 1) 55, 212, 1331, 4565
Page 50 and 51: Restriction enzymes and restriction
Page 52 and 53: Construction of ‘ideal’ vectors
Page 54 and 55:
Cloning into plasmids
Page 58 and 59:
Once you have a recombinant “gene
Page 60 and 61:
Vector and insert size Vector type
Page 63:
Larger inserts, eg, from euk genes:
Page 68 and 69:
Characterization of inserts: Nuclei
Page 70 and 71:
Characterization of inserts: Nuclei
Page 72 and 73:
RE digest, gel electrophoresis reso
Page 74 and 75:
Hybridization results: Comparison o
Page 76 and 77:
•http://www.ncbi.nlm.nih.gov/book
Page 78 and 79:
Zoo blots: Comparative genomics, pr
Page 80 and 81:
Second generation nucleic acids pro
Page 83 and 84:
Biochemistry techniques into molecu
Page 85 and 86:
Northern blot analysis: increased e
Page 87 and 88:
Deletion analysis of binding domain
Page 89 and 90:
Genomics and the ‘new’ Big biol
Page 91 and 92:
Examples of pathway mutations and p
Page 93:
Use of double mutants to order step
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Lecture 7 - Genome Tools

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