Molecular characterisation of odontoblast during primary, secondary ...

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University of Birmingham Research Archive e-theses repository This unpublished thesis/dissertation is copyright of the author and/or third parties. The intellectual property rights of the author or third parties in respect of this work are as defined by The Copyright Designs and Patents Act 1988 or as modified by any successor legislation. Any use made of information contained in this thesis/dissertation must be in accordance with that legislation and must be properly acknowledged. Further distribution or reproduction in any format is prohibited without the permission of the copyright holder.
ACKNOWLEDGEMENTS I wish to express my deepest thanks to my supervisors, Pr Ariane Berdal, Dr Paul Cooper and Professor Tony Smith, for their advice, support and invaluable knowledge throughout my studies. I wish to express my thanks and respects to Pr G. Bergenholtz and Pr H.Magloire for having accepted to juge this work with indulgence. I wish to express my thanks and respects to Pr P. Machtou, Pr D.Maquin, Dr H. Lesot and Dr D. Shelton for their participation as members of the jury. This work was supported by grants from the European Society of Endodontics (ESE) (Annual Research Grant 2004), from the International Federation of Endodontic Association (IFEA) (Annual Research Grant 2005) and from the British Endodontic Society (BES) (Annual research grant 2009) We would like to thank the School of Dentistry of Birmingham and his Dean, Professor P.J Lumley for the financial support. We would like to thank Dr Larry Fisher (National Institute of health) for freely providing Anti Mouse Dentin SialoPhosphoProtein Antibody (ref LR153). 1
Page 1: ECOLE DOCTORALE : Génétique, Cell
Page 5 and 6: Abstract : This research aimed to i
Page 7 and 8: CONTENTS List of Figures ………
Page 9 and 10: 4.5.2 MSX2 and pulp repair ........
Page 11 and 12: Figure 11 : Dentine bridge formatio
Page 13 and 14: minutes. (DMP = dentine matrix prot
Page 15 and 16: lipofectamin ® vector only (MDPC v
Page 17 and 18: List of Tables: Table 1: Primer det
Page 19 and 20: 1.1 Towards a biological approach I
Page 21 and 22: Introduction odontoblast processes
Page 23 and 24: Introduction Secondary dentine: thi
Page 25 and 26: Introduction Understanding the dist
Page 27 and 28: Introduction terms of the communica
Page 29 and 30: Figure 5 : There is a differential
Page 31 and 32: Introduction Movement of the dentin
Page 33 and 34: 1.2.3.3 Consequences of new technol
Page 35 and 36: Introduction an apical nucleus, whe
Page 37 and 38: Introduction creates a physical bar
Page 39 and 40: Introduction polarisation and the d
Page 41 and 42: Introduction variations in pressure
Page 43 and 44: Introduction also express neurogeni
Page 45 and 46: 1.4.1 Reactionary dentinogenesis In
Page 47 and 48: Introduction responses, including a
Page 49 and 50: Introduction superficial cells move
Page 51 and 52: Introduction induce dentine bridge
Page 53 and 54:
Introduction Because of its highly
Page 55 and 56:
Introduction (Hayashi 1982). At 11
Page 57 and 58:
Introduction To date, involvement o
Page 59 and 60:
Introduction In deeper cavities, wh
Page 61 and 62:
Introduction dental papilla cells.
Page 63 and 64:
2 Materials and Methods Material an
Page 65 and 66:
Material and methods containing; 12
Page 67 and 68:
2.1.2.3 Microarray analysis Materia
Page 69 and 70:
Material and methods 2.2 Part Two:
Page 71 and 72:
Material and methods a Modulyo free
Page 73 and 74:
Material and methods Figure 16: Pri
Page 75 and 76:
Material and methods To date, sever
Page 77 and 78:
Material and methods (pH=6.8) with
Page 79 and 80:
Material and methods (P), and calci
Page 81 and 82:
Material and methods (Sigma, la Ver
Page 83 and 84:
Material and methods visualized by
Page 85 and 86:
2.4.2.6 MSX2 over-expression in imm
Page 87 and 88:
Material and methods channel. Data
Page 89 and 90:
Results Amelogenin, IBSP, DCN, Alka
Page 91 and 92:
Results data previously reported fo
Page 93 and 94:
91 MAPK PATHWAY probe set GENE ID b
Page 95 and 96:
Results Alkaline phosphatase and am
Page 97 and 98:
Figure 24: Mouse upper incisor (A)
Page 99 and 100:
Figure 25: Phosphorylated p38 prote
Page 101 and 102:
3.3 MSX2 proteins and dentinogenesi
Page 103 and 104:
Figure 29: First upper molar in +/+
Page 105 and 106:
3.3.1.3 At 21days post natal : Resu
Page 107 and 108:
Results expression, obtained using
Page 109 and 110:
Figure 37: Immunohistochemical anal
Page 111 and 112:
3.3.4 BrdU IHC Results BrdU was inj
Page 113 and 114:
3.3.6 Quantitative RT-PCR (Q-RT-PCR
Page 115 and 116:
Results 3.4 Design and use of a new
Page 117 and 118:
Results Figure 46: Histology at 2 w
Page 119 and 120:
Results cells is also characteristi
Page 121 and 122:
4 Discussion Discussion 4.1 Regulat
Page 123 and 124:
Discussion odontoblast secretory be
Page 125 and 126:
Discussion 4.2 Molecular characteri
Page 127 and 128:
Discussion al. 2004; Magloire, Coub
Page 129 and 130:
Discussion differences. Bone cells
Page 131 and 132:
Discussion we can conclude that the
Page 133 and 134:
Discussion pathway is complex and i
Page 135 and 136:
Discussion odontoblast physiologica
Page 137 and 138:
Discussion dentinogenesis. By perfo
Page 139 and 140:
Discussion cellular and molecular s
Page 141 and 142:
Discussion Notably, our results are
Page 143 and 144:
Discussion contribute to reparative
Page 145 and 146:
Discussion other species (Abedi, To
Page 147 and 148:
Discussion dentinogenesis, some cel
Page 149 and 150:
Discussion morphology. It was not p
Page 151 and 152:
5 Conclusion Discussion Research on
Page 153 and 154:
Discussion Figure 50: 14 months pos
Page 155 and 156:
References during normal and in vit
Page 157 and 158:
References Faraco, I. M., Jr. and R
Page 159 and 160:
References Kidd, E. A., A. Banerjee
Page 161 and 162:
References antigen-expressing cells
Page 163 and 164:
References Smith, A. J. and H. Leso
Page 165 and 166:
References Yamashiro, T., M. Tummer
Page 167 and 168:
References Appendix 1: Full list of
Page 169 and 170:
probe set GENE ID baseline mean (LS
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Appendix 3: Molecular characterizat
Page 193:
Appendix 5: Evaluation of a new lab
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