(Converted)-4 - Journal of Cell and Molecular Biology - Haliç ...

More documents

Recommendations

Info

28 Canan Can et al. Table 1: Interaction phenotypes of different P. parasitica isolates on the A. thaliana ecotypes. Cand-5, Hind-2 and Hind-4 isolates recognize MRC-B and MRC-C region which present at the second chromosome in the Col-5 ecotype. The isolates regenerated from the oospore populations were inoculated on different A. thaliana ecotypes (Col-5, Ksk-1, Nd-1, Ws-3, Ler-1, Oy-1 and Wei-1) to determine if they were original. The results are shown in Table1. As it could be seen in Table 1, the isolates generated from the oospore populations were found to be as original, and there was no variation (Can, 1997). Therefore these isolates were used to inoculate the mutant lines recovered through inoculation with the Hiks-1 isolate. Interaction phenotypes on different A. thaliana ecotypes* P. parasitica isolates Col-5 Ksk-1 Nd-1 Ws-3 Ler-1 Oy-1 Wei-1 Hiks-1 FN EH PN PN FN DM FDL Ahco-1 DM FN FDL FN FDL FN FDL Ahco-2 DM FN FDL FN FN FN DM Ahco-7 DM FN FDL FN FDL FN EH Wand-1 FN FN EH FN FN FN DM Cand-5 FN EH EH FN CN FDL DL Hind-2 FN FN EH PN FN FN EH Hind-4 FR FN EH PN EH FN EH *Necrotic pits (PN), necrotic flecks (FN), cavities (CN), flecks with delate and moderate pathogen sporulation, 1-20 sporangiophorus per each cotyledon (DM), flecking with delate pathogen sporulation, 5-10 sporangiophorus per each cotyledon (FDL), early and heavy pathogen sporulation, 20> sporangiophores per cotyledon (EH). Phenotypic characterization of mutant lines P. parasitica isolates were used to inoculate the Col-5 lines. The results were shown in Table 2. As indicated in Table 1, DM and EH phenotypes developed, following inoculation of FN3922, FN3928, FN3929 and FN3930 mutant lines with the Hiks-1 isolate. These results revealed that the RPP7 gene is not present in the mutant lines. However, Ahco-1, Ahco-2 and Ahco-7 isolates exhibited the EH phenotype compared to DM in the wild Col-5 ecotype. This result showed that absence of the RPP7 gene increased susceptibility. The important point in here was that mutation of one R-gene could effect the resistance in same plant to other isolate. The result Table 2: Interaction phenotypes exhibited by Col-5 mutant lines following inoculation with different P. parasitica isolates. The interaction phenotypes on wild type and mutant lines* P. parasitica isolates Col-5 Nd-1 Ksk-1 FN3922 FN3928 FN3929 FN3930 Hiks-1 FN PN EH EH DM EH EH Ahco-1 DM FDL FN EH EH EH EH Ahco-2 DM FDL FN EH EH EH EH Ahco-7 DM FDL FN EH EH EH EH Wand-1 FN EH FN EH FN FN FDL Cand-5 FN EH EH EH FN FN FN Hind-2 FN EH FN FN FN FN FN Hind-4 FR EH FN EH FN FN FN *Necrotic pits (PN), necrotic flecks (FN), flecks with moderate and late pathogen sporulation, 1-20 sporangiophorus per each cotyledon (DM), flecking with delate pathogen sporulation, 5-10 sporangiophorus per each cotyledon (FDL), early and heavy pathogen sporulation, 20> sporangiophores per cotyledon (EH).
Figure 1: Microscopic reactions occurred in Col-5 ecotype following inoculation with the Hiks-1 isolate. (A) Six hours after inoculation. a. Haustorium formed in the mesofil cells. b. Cell death and (B) Twelve hours after inoculation. Hypersensitive reaction and cell death. 750 bp → 500 bp → Figure 3: Distribution of g2a markers in mutant lines. M indicates the 1 kb DNA marker. may also show that when the RPP genes are allelic they do function in coordination. Cand-5 isolate recognizes MRC-B region which is present in Col-5 and determined with FN interaction phenotype. FN3922 mutant line exhibited the EH phenotype with Cand-5 and FN with other isolates tested (Table 2). This result may show that FN3922 may be a Col-5 contamination. On the other word it is possible that in this line, many RPP genes present in the MRC-B loci (Can, 1997) could be mutated. Therefore, FN3922 may not be a specific mutant of the RPP7 gene. As it is known the Fast Neutron application results point mutations, so that at MRC-B region in FN3922, many point mutations or deletions may have been occurred. Inoculations of the FN3928, FN3929 and FN3930 mutant lines with Wand-1, Hind-2, Hind-4 resulted FN phenotype. This results shows that the RPP7 gene has no correlation with the RPP genes present in seconder chromosome at the MRC-C region (Table 2). Microscopic characterization of mutant lines The fungal development in plant tissue was examined. Following inoculations at the first, third and seven days the cotyledons from the mutant lines and the wild type Col-5 were taken and prepared as described before. At the first six hour of inoculation of Col-5 with the Hiks-1 isolate cell death was observed. The susceptible genotypes and the mutant lines allowed the penetration and mycelial development without any cell (Figure 1, 2). Fungal development in the mutant lines supported the macroscopic results (Mc Dowell et al., 2000). DNA analyses of mutant lines Characterisation of RPP7 gene 29 Figure 2: Microscopic reactions occured the FN3929 mutant line following inoculation with the Hiks-1 isolate. Formation of fungal haustorium in the mesophyl cell (a) and hif development (b), twelve hours after inoculation. nga280 and g2a markers present at the lower arm of the first chromosome of A. thaliana were detected to give less recombination with the RPP7 loci (Can, 1997). Therefore the mutant lines were subjected to PCR analyses with these markers. Results are given in Figure 3. The distributions of the bands in mutant lines were similar to those in the wild type Col-5. This result may suggest that no deletions may occurred in the mutant lines and that the loss of function could be due to a point mutation.
Page 1 and 2: ‹STANBUL 1998 VOLUME 2 • NO. 1
Page 3 and 4: Journal of Cell and Molecular Biolo
Page 7 and 8: Arthur W. Galston, Curriculum Vitae
Page 9 and 10: 2 Ravindar Kaur-Sawhney et al. 1. I
Page 11 and 12: 4 Ravindar Kaur-Sawhney et al. and
Page 13 and 14: 6 Ravindar Kaur-Sawhney et al. been
Page 15 and 16: 8 Ravindar Kaur-Sawhney et al. incr
Page 17 and 18: 10 Ravindar Kaur-Sawhney et al. Pro
Page 19 and 20: 12 Ravindar Kaur-Sawhney et al. phe
Page 21 and 22: 14 Valentine I. Kefeli et al. molec
Page 23 and 24: 16 Valentine I. Kefeli et al. Hence
Page 25 and 26: 18 Valentine I. Kefeli et al. is an
Page 27 and 28: 20 Tülin Aktaç et al. their econo
Page 29 and 30: 22 Tülin Aktaç et al. Table 1: Ef
Page 33: little plastic pots containing 4:1:
Page 37 and 38: Introduction Journal of Cell and Mo
Page 39 and 40: Figure 2: The effect of meta-topoli
Page 43 and 44: 1 2 3 4 5 6 7 8 9 10 11 12 Figure 1
Page 47 and 48: Figure 1: The partial karyotype of
Page 51 and 52: Mitotic index analysis Mitotic inde
Page 53 and 54: seem to be concordant with the abov
Page 55 and 56: Letter to editor P rostate cancer a
Page 57 and 58: circulating tHcy can be detected in
Page 59 and 60: Instructions for authors General 1.
Page 61: Journal of Cell and Molecular Biolo

(Converted)-4 - Journal of Cell and Molecular Biology - Haliç ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?