DNA & RNA TRANSFECTION REAGENTS - BioNova
DNA & RNA TRANSFECTION REAGENTS - BioNova
DNA & RNA TRANSFECTION REAGENTS - BioNova
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<strong>DNA</strong> & <strong>RNA</strong> <strong>TRANSFECTION</strong> <strong>REAGENTS</strong><br />
Broad Spectrum Delivery<br />
TransIT ® -LT1 Reagent<br />
TransIT ® -LT2 Reagent<br />
TransIT ® -Express Reagent<br />
<strong>DNA</strong> si<strong>RNA</strong> <strong>RNA</strong> OLIGOS<br />
Cell Line Specific Delivery<br />
HEK 293<br />
3T3<br />
CHO<br />
COS<br />
HeLa<br />
Insect (Sf9)<br />
Jurkat<br />
Keratinocyte<br />
Neural<br />
Prostate<br />
si<strong>RNA</strong> Delivery Viral <strong>RNA</strong> and m<strong>RNA</strong> <strong>DNA</strong> and <strong>RNA</strong><br />
TransIT ® -TKO Reagent<br />
TransIT-siQUEST ® Reagent<br />
NEW<br />
TransIT ® -siPAK Kits<br />
si<strong>RNA</strong> Localization<br />
Label IT ® si<strong>RNA</strong> Tracker Kits<br />
Label IT ® <strong>RNA</strong>i Delivery Controls<br />
TransIT ® -m<strong>RNA</strong> Kit<br />
TransIT ® -Oligo Reagent<br />
mirusbio.com
Plasmid Transfection: Broad Spectrum & Cell Line Specific<br />
Plasmid transfection into mammalian cells is a crucial step in many experiements and requires<br />
transfection reagents that deliver plasmids without perturbing cell physiology. To<br />
meet these needs, Mirus Bio developed two different types of plasmid transfection reagents.<br />
TransIT ® -LT1 Reagent is a broad spectrum plasmid transfection reagent that enables high efficiency<br />
transfection into a wide variety of cells. The other TransIT Cell Line Specific Reagents<br />
are optimized to produce increased transfection efficiency compared to TransIT-LT1 Reagent in<br />
popular or hard-to-transfect cell lines.<br />
Benefits of the TransIT Transfection Reagents Include:<br />
Ease-of-Use: Simple protocols with easy optimization mean you spend less time optimizing<br />
and more time doing the important experiments.<br />
Low Cellular Toxicity: Maintains healthy cell density while reducing potential experimental<br />
biases due to toxicity.<br />
High Efficiency Transfection: High percentage of the cells expressing transfected plasmid ensures<br />
experimental success.<br />
Transfects Single or Multiple Plasmids: Enables use of reagents in a variety of applications including<br />
viral production, transcriptional regulation, and si<strong>RNA</strong>/sh<strong>RNA</strong> expression.<br />
Easy-to-Use and Serum Compatible<br />
1. Dilute TransIT ®<br />
Transfection<br />
Reagent in serumfree<br />
media, mix<br />
Protocol Overview for TransIT Transfection Reagents. TransIT Transfection Kits contain two components and require<br />
an additional mixing step when using these kits.<br />
Low Cellular Toxicity<br />
2. Add purified<br />
nucleic acid,<br />
mix and incubate.<br />
3. Add complexes<br />
directly to cells<br />
in serum<br />
containing media.<br />
4. Incubate.<br />
and incubate. 24-48 hours Perform<br />
Assay<br />
Comparative Toxicity of TransIT-LT1 Transfection<br />
Reagent and Another Leading<br />
Transfection Reagent in COS-7 Cells. In<br />
a transfection experiment, cells that are<br />
rounding up and peeling off the surface of<br />
the plate characterize cellular toxicity. The<br />
photos compare toxicity among nontransfected<br />
cells, cells transfected with<br />
Nontransfected TransIT<br />
TransIT-LT1 Reagent, and cells transfected with another leading transfection reagent. Each plate was treated following<br />
the manufacturers’ recommended protocol: the only variable was the type of transfection reagent used.<br />
TransIT-LT1 Reagent achieves high transfection efficiency while maintaining healthy cells.<br />
® -LT1 Reagent Competitor<br />
www.mirusbio.com<br />
BROAD SPECTRUM & CELL LINE SPECIFIC <strong>REAGENTS</strong> <strong>TRANSFECTION</strong>
<strong>RNA</strong> Transfection: The Broad Spectrum TransIT-m<strong>RNA</strong> Kit<br />
The transfection of <strong>RNA</strong> molecules into mammalian cells can be used for a variety of experimental<br />
purposes such as rapid, short-term protein expression after m<strong>RNA</strong> transfection or production<br />
of infectious <strong>RNA</strong> virus after transfection of the viral genomic <strong>RNA</strong>. Both applications require a<br />
transfection reagent that delivers <strong>RNA</strong> molecules with high efficiency and low cellular toxicity.<br />
Mirus Bio developed the TransIT-m<strong>RNA</strong> Transfection Kit for the high efficiency, low toxicity transfection<br />
of m<strong>RNA</strong> and viral <strong>RNA</strong>s.<br />
Benefits of TransIT-m<strong>RNA</strong> Transfection Kit Over Other Commercially Available Reagents:<br />
Ease-of-Use: A simple protocol with no required media changes saves valuable time.<br />
Low Cellular Toxicity: Maintains cell density and reduces the introduction of experimental<br />
biases.<br />
High Efficiency Transfection: Transfection of a large percentage of the cell population ensures<br />
experimental success.<br />
Serum Compatible: Transfections are performed in the presence of serum, which eliminates<br />
media changes and enhances cell viability.<br />
Transfects Various Sizes of <strong>RNA</strong>: Ideal for applications such as <strong>RNA</strong> virus production and<br />
short-term protein expression from transfected m<strong>RNA</strong>.<br />
High Efficiency Transfection Using the TransIT-m<strong>RNA</strong> Kit<br />
Percent EGFP Expressing Cells<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
CHO-K1<br />
COS-7<br />
HEK 293<br />
HeLa<br />
The TransIT-m<strong>RNA</strong> Transfection<br />
Kit Efficiently Delivers m<strong>RNA</strong> to a<br />
Variety of Cell Lines. The indicated<br />
cell lines were transfected with<br />
0.5 µg of a capped, polyadenylated<br />
in vitro transcript encoding EGFP<br />
using the TransIT-m<strong>RNA</strong> Transfection<br />
Kit. Eighteen hours post-transfection,<br />
the cells were analyzed by<br />
flow cytometry to identify the EGFP<br />
expressing cells.<br />
Nontransfected lacZ m<strong>RNA</strong> Transfected<br />
The TransIT-m<strong>RNA</strong> Transfection Kit Efficiently Delivers lacZ m<strong>RNA</strong> to<br />
CHO-K1 Cells. Using the TransIT-m<strong>RNA</strong> Transfection Kit, CHO-K1 cells<br />
were mock transfected (A) or transfected with a capped and polyadenylated<br />
lacZ encoding m<strong>RNA</strong> (B). Approximately 18 hrs post-transfection the<br />
cells were stained using Mirus Bio’s Beta-gal Staining Kit to identify the<br />
lacZ expressing cells.<br />
www.mirusbio.com<br />
<strong>TRANSFECTION</strong> BROAD SPECTRUM & CELL LINE SPECIFIC <strong>REAGENTS</strong>
TransIT-LT1 Transfects a Wide Array of Cell Lines with High Efficiency<br />
Percentage of EGFP Expressing Cells<br />
100<br />
80<br />
60<br />
40<br />
20<br />
0<br />
A549<br />
CHO-K1<br />
COS-7<br />
HEK 293<br />
HeLa<br />
HepG2<br />
Keratinocytes<br />
LNCaP<br />
Neuro-2a<br />
NIH 3T3<br />
PC-3<br />
Maximum Transfection Efficiency in Popular or Hard-to-Transfect Cells<br />
Using the TransIT Cell Line Specific Reagents and Kits<br />
Ideal for a Variety of Applications<br />
TransIT-LT1 Transfection Reagent Efficiency on Various<br />
Cell Lines. Various cell lines were transfected using-<br />
TransIT-LT1 Reagent and the pEGFP expression vector<br />
in complete growth media with no media changes.<br />
Cell Line(s) Cell Line Specific Reagent Transfection Efficiency<br />
HEK 293 TransIT-293 Transfection Reagent 75-85%<br />
NIH 3T3 TransIT-3T3 Transfection Kit 55-65%<br />
HeLa TransIT-HelaMONSTER Transfection Kit 50-60%<br />
CHO-K1 TransIT-CHO Transfection Kit 50-60%<br />
COS-1 and COS-7 TransIT-COS Transfection Kit 90-95%<br />
Jurkat TransIT-Jurkat Transfection Reagent 10-25%<br />
DU 145, LNCaP, PC-3 TransIT-Prostate Transfection Kit 50%<br />
Neuro-2a TransIT-Neural Transfection Reagent 75%<br />
Sf9 TransIT-Insecta Transfection Reagent 40-60%<br />
Keratinocytes TransIT-Keratinocyte Transfection Reagent 20-30%<br />
Gene Expression<br />
Protein expression for phenotype analysis or purification<br />
<strong>RNA</strong> processing studies<br />
Transcriptional Regulation<br />
Promoter analysis<br />
Transcription factor analysis<br />
Viral Production/Rescue<br />
Creation of viruses or virus-like particles by plasmid transfection<br />
Retrovirus, lentivirus, influenza, ebola, HDV, SV40, VSV, and Hepatitis B<br />
<strong>RNA</strong>i Target Gene Knockdown<br />
Induction of <strong>RNA</strong>i pathway by transfection of si<strong>RNA</strong>, sh<strong>RNA</strong>, or pri-mi<strong>RNA</strong> expression vectors<br />
www.mirusbio.com
Cell Lines Transfected with TransIT-LT1 Reagent at Mirus Bio<br />
A549, BHK-21, BNL.CL2, BRL-3A, C2C12, C6*, CHO-K1*, Clone 9, COS-1*,<br />
COS-7*, Daoy*, DB-TRG-05MG, DI-TNC1*, DU 145*, HEK 293*, HeLa*, Hepa 1-6, Hepa-<br />
1cLc, HepG2, HLF-a, Huh-7, HUVEC*, Jurkat*, K562*, KB, KLN 205, LL/2 (LLC1),<br />
LNCaP*, MCF-7, Neuro-2a*, NIH3T3*, OVCAR3, PC3*, PC-12*, RAW 264.7*,<br />
SK-N-MC*, SKOV3, SVGp12*, SW900, THP-1*, Vero, and WRL-68<br />
Primary Cell Types<br />
Human astrocytes*, human chondrocytes*, human keratinocytes*, mouse and rat<br />
hepatocytes*<br />
*TransIT Cell Line Specific Transfection Reagents are recommended for these cell types.<br />
Cell Lines Transfected with TransIT-LT1 Reagent and<br />
Referenced in Published Citations<br />
5637, A2780, A549, ACHN, BAEC, BALB/3T3, BC-2, BC-3, BeWo, BHK-21, BPAE, C2C12,<br />
C3H/10T1/2, CF2, Cf2Th, Chicken Embryo Chondrocytes, Chicken Gizzard SMC, CHO-K1, COS-<br />
7, CV-1, DG-75, DLD-1, EA.hy926, EL2, Embryonic Rat Lung Fibroblasts, ES2, Extravillous Cytotrophoblast,<br />
Fibroblasts, GH4, GIP-Ins, H19-7, HaCat, HAß18, HCT 116, HEK 293, HEK 293E,<br />
HEK 293T, HeLa, HeLa S2, HeLa S3, Hep3C, HepG2, HFF, HM3, Hs578T, HT-1080, HuH-7,<br />
Human Bone Marrow EC Line, HUVEC, IF5, IG10, INS-1, JEG-3, K562, KMCH-1, L6, LC319,<br />
LMH, MA-10, MC3T3, MCF-7, MDA-MB-231, MDA-MB-468, MDCK, Mesenchymal Chondrorogenitor<br />
Cells, MIN6, Monocytes, Mouse Brain Endothelial Cells, Mouse Embryo Fibroblasts,<br />
Mouse Mesangial Cells, Mouse Myoblasts, MOVCAR, Mv1Lu, Myometrial, Mz-ChA-1, NCI-H23,<br />
NCM460, Neuro-2a, Neuroepithelial, NG108-15, NIH3T12, NIH3T3, NRK, NS20Y, NuTu26,<br />
Osteoblasts, OVCAR10, OVCAR3, P19, P388D1 ,PANC-1, PC-12, PC3, PH5CH8, PlateE, Primary<br />
Bovine Luteal Cells, Primary Human Keratinocytes, Primary Human Lung Fibroblasts, Primary<br />
Mouse Myotubes, Primary Rat Aortic Cells-SMCs, PT67, PtK2, QT6, Rabbit Corneal Epithelial<br />
Cells, Raji, Rat Hepatocyte Clone 9, Rat ß Cells, Rat-1, RAW 264.7, RCJ3.IC5.18, RD, RFL-6,<br />
RLMEC, ROS-17/2.8, RXF631L, Saos, SK-N-SH, SR40, TE14, tEnd.1, Tera-2, TIG-7, U266,<br />
U2OS, UMR-106, Vero, Y1<br />
www.mirusbio.com<br />
<strong>TRANSFECTION</strong> BROAD SPECTRUM & CELL LINE SPECIFIC <strong>REAGENTS</strong>
Order Information<br />
Cell Line Specific Plasmid Transfection<br />
Product Name Product # Quantity<br />
TransIT ® -293 MIR 2704 0.4ml<br />
Transfection Reagent MIR 2700 1 ml<br />
MIR 2705 5 X 1 ml<br />
MIR 2706 10 X 1 ml<br />
TransIT ® -3T3 MIR 2184 0.4 ml<br />
Transfection Kit* MIR 2180 1 ml<br />
MIR 2185 5 X 1 ml<br />
MIR 2186 10 X 1 ml<br />
TransIT ® -CHO MIR 2174 0.4 ml<br />
Transfection Kit* MIR 2170 1 ml<br />
MIR 2175 5 X 1 ml<br />
MIR 2176 10 X 1 ml<br />
TransIT ® -COS MIR 2194 0.4 ml<br />
Transfection Kit* MIR 2190 1 ml<br />
MIR 2195 5 X 1 ml<br />
MIR 2196 10 X 1 ml<br />
TransIT ® -HeLaMONSTER ® MIR 2904 0.4 ml<br />
Transfection Kit* MIR 2900 1 ml<br />
MIR 2905 5 X 1 ml<br />
MIR 2906 10 X 1 ml<br />
TransIT ® -Insecta MIR 2204 0.4 ml<br />
Transfection Reagent MIR 2200 1 ml<br />
MIR 2205 5 X 1 ml<br />
MIR 2206 10 X 1 ml<br />
TransIT ® -Jurkat MIR 2124 0.4 ml<br />
Transfection Reagent MIR 2120 1 ml<br />
MIR 2125 5 X 1 ml<br />
MIR 2126 10 X 1 ml<br />
TransIT ® -Keratinocyte MIR 2804 0.4 ml<br />
Transfection Reagent MIR 2800 1 ml<br />
MIR 2805 5 X 1 ml<br />
MIR 2806 10 X 1 ml<br />
TransIT-Neural ® MIR 2144 0.4 ml<br />
Transfection Reagent MIR 2140 1 ml<br />
MIR 2145 5 X 1 ml<br />
MIR 2146 10 X 1 ml<br />
TransIT ® -Prostate MIR 2134 0.4 ml<br />
Transfection Kit* MIR 2130 1 ml<br />
MIR 2135 5 X 1 ml<br />
MIR 2136 10 X 1 ml<br />
TransIT ® Transfection Kits are supplied with a transfection reagent and a booster reagent.<br />
www.mirusbio.com<br />
Broad Spectrum Plasmid Transfection<br />
Product Name Product # Quantity<br />
TransIT ® -LT1 MIR 2304 0.4 ml<br />
Transfection Reagent MIR 2300 1 ml<br />
MIR 2305 5 X 1 ml<br />
MIR 2306 10 X 1 ml<br />
TransIT ® -LT2 MIR 2404 0.4 ml<br />
Transfection Reagent MIR 2400 1 ml<br />
MIR 2405 5 X 1 ml<br />
MIR 2406 10 X 1 ml<br />
TransIT ® -Express MIR 2004 0.4 ml<br />
Transfection Reagent MIR 2000 1 ml<br />
MIR 2005 5 X 1 ml<br />
MIR 2006 10 X 1 ml<br />
m<strong>RNA</strong> Transfection<br />
Product Name Product # Quantity<br />
TransIT ® -m<strong>RNA</strong> MIR 2225 0.25 ml<br />
Transfection Kit* MIR 2250 0.5 ml<br />
MIR 2255 5 X 0.5 ml<br />
MIR 2256 10 X 0.5 ml<br />
Oligonucleotide Transfection<br />
Product Name Product # Quantity<br />
TransIT ® -Oligo MIR 2164 0.4 ml<br />
Transfection Reagent MIR 2160 1 ml<br />
MIR 2165 5 X 1 ml<br />
MIR 2166 10 X 1 ml<br />
Labeled Plasmid Delivery Control<br />
Product Name Product # Quantity<br />
Label IT ® Plasmid MIR 7904 10 g<br />
Delivery Control, Cy3<br />
Label IT<br />
MIR 7905 100 g<br />
® Plasmid<br />
Delivery Control, MIR 7906 10 g<br />
Fluorescein MIR 7907 100 g<br />
TFXFL06 7/2006<br />
© 2006, Mirus Bio Corporation. HelaMONSTER, HybQUEST, Label IT, MiraCLEAN,<br />
siQUEST, siXpress, TransIT, TransIT-Neural and TransIT-TKO are registered trademarks<br />
of Mirus Bio Corporation. Label IT Tracker is a trademark of Mirus Bio Corporation.<br />
Cy3 and Cy5 are trakemarks of GE Healthcare. The Label IT ® Cy3 and<br />
Cy5 products or portions there of are manufactured under license from Carnegie<br />
Mellon University and are protected by U.S. patent #5,268,486 and other patents pending.<br />
<strong>TRANSFECTION</strong> BROAD SPECTRUM & CELL LINE SPECIFIC <strong>REAGENTS</strong>
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