DNA & RNA TRANSFECTION REAGENTS - BioNova

TransIT-LT1 Transfects a Wide Array of Cell Lines with High Efficiency
Percentage of EGFP Expressing Cells
100
80
60
40
20
0
A549
CHO-K1
COS-7
HEK 293
HeLa
HepG2
Keratinocytes
LNCaP
Neuro-2a
NIH 3T3
PC-3
Maximum Transfection Efficiency in Popular or Hard-to-Transfect Cells
Using the TransIT Cell Line Specific Reagents and Kits
Ideal for a Variety of Applications
TransIT-LT1 Transfection Reagent Efficiency on Various
Cell Lines. Various cell lines were transfected using-
TransIT-LT1 Reagent and the pEGFP expression vector
in complete growth media with no media changes.
Cell Line(s) Cell Line Specific Reagent Transfection Efficiency
HEK 293 TransIT-293 Transfection Reagent 75-85%
NIH 3T3 TransIT-3T3 Transfection Kit 55-65%
HeLa TransIT-HelaMONSTER Transfection Kit 50-60%
CHO-K1 TransIT-CHO Transfection Kit 50-60%
COS-1 and COS-7 TransIT-COS Transfection Kit 90-95%
Jurkat TransIT-Jurkat Transfection Reagent 10-25%
DU 145, LNCaP, PC-3 TransIT-Prostate Transfection Kit 50%
Neuro-2a TransIT-Neural Transfection Reagent 75%
Sf9 TransIT-Insecta Transfection Reagent 40-60%
Keratinocytes TransIT-Keratinocyte Transfection Reagent 20-30%
Gene Expression
Protein expression for phenotype analysis or purification
RNA processing studies
Transcriptional Regulation
Promoter analysis
Transcription factor analysis
Viral Production/Rescue
Creation of viruses or virus-like particles by plasmid transfection
Retrovirus, lentivirus, influenza, ebola, HDV, SV40, VSV, and Hepatitis B
RNAi Target Gene Knockdown
Induction of RNAi pathway by transfection of siRNA, shRNA, or pri-miRNA expression vectors
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