DNA & RNA TRANSFECTION REAGENTS - BioNova

More documents

Recommendations

Info

Plasmid Transfection: Broad Spectrum & Cell Line Specific Plasmid transfection into mammalian cells is a crucial step in many experiements and requires transfection reagents that deliver plasmids without perturbing cell physiology. To meet these needs, Mirus Bio developed two different types of plasmid transfection reagents. TransIT ® -LT1 Reagent is a broad spectrum plasmid transfection reagent that enables high efficiency transfection into a wide variety of cells. The other TransIT Cell Line Specific Reagents are optimized to produce increased transfection efficiency compared to TransIT-LT1 Reagent in popular or hard-to-transfect cell lines. Benefits of the TransIT Transfection Reagents Include: Ease-of-Use: Simple protocols with easy optimization mean you spend less time optimizing and more time doing the important experiments. Low Cellular Toxicity: Maintains healthy cell density while reducing potential experimental biases due to toxicity. High Efficiency Transfection: High percentage of the cells expressing transfected plasmid ensures experimental success. Transfects Single or Multiple Plasmids: Enables use of reagents in a variety of applications including viral production, transcriptional regulation, and siRNA/shRNA expression. Easy-to-Use and Serum Compatible 1. Dilute TransIT ® Transfection Reagent in serumfree media, mix Protocol Overview for TransIT Transfection Reagents. TransIT Transfection Kits contain two components and require an additional mixing step when using these kits. Low Cellular Toxicity 2. Add purified nucleic acid, mix and incubate. 3. Add complexes directly to cells in serum containing media. 4. Incubate. and incubate. 24-48 hours Perform Assay Comparative Toxicity of TransIT-LT1 Transfection Reagent and Another Leading Transfection Reagent in COS-7 Cells. In a transfection experiment, cells that are rounding up and peeling off the surface of the plate characterize cellular toxicity. The photos compare toxicity among nontransfected cells, cells transfected with Nontransfected TransIT TransIT-LT1 Reagent, and cells transfected with another leading transfection reagent. Each plate was treated following the manufacturers’ recommended protocol: the only variable was the type of transfection reagent used. TransIT-LT1 Reagent achieves high transfection efficiency while maintaining healthy cells. ® -LT1 Reagent Competitor www.mirusbio.com BROAD SPECTRUM & CELL LINE SPECIFIC REAGENTS TRANSFECTION
RNA Transfection: The Broad Spectrum TransIT-mRNA Kit The transfection of RNA molecules into mammalian cells can be used for a variety of experimental purposes such as rapid, short-term protein expression after mRNA transfection or production of infectious RNA virus after transfection of the viral genomic RNA. Both applications require a transfection reagent that delivers RNA molecules with high efficiency and low cellular toxicity. Mirus Bio developed the TransIT-mRNA Transfection Kit for the high efficiency, low toxicity transfection of mRNA and viral RNAs. Benefits of TransIT-mRNA Transfection Kit Over Other Commercially Available Reagents: Ease-of-Use: A simple protocol with no required media changes saves valuable time. Low Cellular Toxicity: Maintains cell density and reduces the introduction of experimental biases. High Efficiency Transfection: Transfection of a large percentage of the cell population ensures experimental success. Serum Compatible: Transfections are performed in the presence of serum, which eliminates media changes and enhances cell viability. Transfects Various Sizes of RNA: Ideal for applications such as RNA virus production and short-term protein expression from transfected mRNA. High Efficiency Transfection Using the TransIT-mRNA Kit Percent EGFP Expressing Cells 100 80 60 40 20 0 CHO-K1 COS-7 HEK 293 HeLa The TransIT-mRNA Transfection Kit Efficiently Delivers mRNA to a Variety of Cell Lines. The indicated cell lines were transfected with 0.5 µg of a capped, polyadenylated in vitro transcript encoding EGFP using the TransIT-mRNA Transfection Kit. Eighteen hours post-transfection, the cells were analyzed by flow cytometry to identify the EGFP expressing cells. Nontransfected lacZ mRNA Transfected The TransIT-mRNA Transfection Kit Efficiently Delivers lacZ mRNA to CHO-K1 Cells. Using the TransIT-mRNA Transfection Kit, CHO-K1 cells were mock transfected (A) or transfected with a capped and polyadenylated lacZ encoding mRNA (B). Approximately 18 hrs post-transfection the cells were stained using Mirus Bio’s Beta-gal Staining Kit to identify the lacZ expressing cells. www.mirusbio.com TRANSFECTION BROAD SPECTRUM & CELL LINE SPECIFIC REAGENTS
Page 1: DNA & RNA TRANSFECTION REAGENTS Bro
Page 5 and 6: Cell Lines Transfected with TransIT

DNA & RNA TRANSFECTION REAGENTS - BioNova

Create successful ePaper yourself

Delete template?

Save as template?