1D and 2D Protein Electrophoresis - Institut de Cancérologie et d ...

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D<strong>et</strong>ection of low abundant phoshorylated protein Actin pp38 Relative intensity (x 10 -5 ) 9 8 7 6 5 4 3 2 1 0 0 2.5 5 15 0 2,5 5 15 TGF-β stimulation time (minutes) Cy3 Increase in p38 activation upon TGF- stimulation for 0, 2,5, 5 or 15 minutes in 293Tcells Cy5
Mr. Delaive Biochimie Cellulaire <strong>de</strong>pt. FUNDP Namur , Belgium
Page 1 and 2: - temperature + + + + + + + + + + +
Page 3 and 4: SDS Polyacrylamide Gel Electrophore
Page 5 and 6: Practical hints Always use Tris bas
Page 7 and 8: Disc electrophoresis in readymade g
Page 9 and 10: Pre-cast PhastGel run in 30 minutes
Page 11 and 12: SDS electrophoresis SDS is an anion
Page 13 and 14: Blotting and Specific Detection
Page 15 and 16: 100ug Gel staining versus blotting
Page 17 and 18: Chemiluminescent Detection - ECL Pr
Page 19 and 20: Protein bound to membrane blocked w
Page 21 and 22: ECL Plex - relative quantitative We
Page 23 and 24: GE Healthcare Life Sciences Imaging
Page 25 and 26: CyDye TM Properties Very bright •
Page 27 and 28: The power of relating to house-keep
Page 29: Detection of proteins from rat brai
Page 33 and 34: Blue Native PAGE For the analysis o
Page 35 and 36: Nonionic detergents for solibilizat
Page 37 and 38: Steps after solubilization Incubati
Page 39 and 40: Buffer system for BN PAGE Blue load
Page 41 and 42: Blue Native- PAGE of Protein Comple
Page 43 and 44: 2D Blue Native/SDS-Electrophoresis
Page 45 and 46: Resolution of mitochondrial superco
Page 47 and 48: 8 th Blue Native Electrophoresis Co
Page 49 and 50: Cationic Detergent PAGE For the ana
Page 51 and 52: Cationic detergent acidic gel PAGE
Page 53 and 54: DIGE: 16-BAC / SDS PAGE gels for hy
Page 55 and 56: Isoelectric Focusing Separation acc
Page 57 and 58: IEF with carrier ampholytes Pharmal
Page 59 and 60: Immobilized pH Gradients (IPG) Acry
Page 61 and 62: Two-dimensional Electrophoresis For
Page 63 and 64: Sample preparation Avoiding protein
Page 65 and 66: SDS in 2-D sample preparation Best
Page 67 and 68: Protein precipitation Clean-up from
Page 69 and 70: -Rat tongue tissue (26ug) -pH 3-10,
Page 71 and 72: Resolubilization possibilities •P
Page 73 and 74: The Trap platform Scale down from m
Page 75 and 76: Protein Enrichment products • NHS
Page 77 and 78: Make your own protein enrichment to
Page 79 and 80: Repeatability study: enrichment of
Page 81 and 82:
Isoelectric Focusing Separation acc
Page 83 and 84:
Principle of 2-D electrophoresis 1.
Page 85 and 86:
Sample loading on pre-rehydrated IP
Page 87 and 88:
IPGphor run recording graphs
Page 89 and 90:
Streaking in basic pH region DTT an
Page 91 and 92:
Micropreparative focusing with DeSt
Page 93 and 94:
Basic gels: pH 7-11 NL New propriet
Page 95 and 96:
SDS PAGE chambers for Proteomics 25
Page 97 and 98:
Casting SDS gels and overlay gel ed
Page 99 and 100:
Wide pH gradient: 3 - 11 NL Mouse l
Page 102 and 103:
Protein Detection Methods Coomassie
Page 104 and 105:
Deep Purple Staining - fluorescent
Page 106 and 107:
Deep Purple - applications 2D gel 2
Page 108 and 109:
Carbamylation trains Impurities in
Page 110 and 111:
Salt in the gel Cell washing with P
Page 112 and 113:
Equilibration with SDS buffer 15 mi
Page 114 and 115:
2D Electrophoresis GE Healthcare Li
Page 116:
Amersham Biosciences AB, a General
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