1D and 2D Protein Electrophoresis - Institut de Cancérologie et d ...

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Solubilization of mitochondrial proteins by Dodecyl maltoside (DDM) and Digitonin I+III 2 I I* V III2 HSP60 V* FDH I+III 2 prohibitin I V III2 HSP60 DDM (1.5 g/g) Digitonin (5 g/g) IVb IVb TOM II FDH From Prof. H.-P. Braun, Abteilung für angewandte Genetik,Universität Hannover
Resolution of mitochondrial supercomplexes from Arabidopsis by 2D Blue-native / Blue-native PAGE I III 2 BN-PAGE (digitonin) I+III 2 I V III 2 BN-PAGE (dodecylmaltoside) I III 2 BN-PAGE (digitonin) I+III 2 I V III 2 Eubel et al. 2003, Plant Physiol. 133, pp 274-286. BN-PAGE (dodecylmaltoside)
Page 1 and 2: - temperature + + + + + + + + + + +
Page 3 and 4: SDS Polyacrylamide Gel Electrophore
Page 5 and 6: Practical hints Always use Tris bas
Page 7 and 8: Disc electrophoresis in readymade g
Page 9 and 10: Pre-cast PhastGel run in 30 minutes
Page 11 and 12: SDS electrophoresis SDS is an anion
Page 13 and 14: Blotting and Specific Detection
Page 15 and 16: 100ug Gel staining versus blotting
Page 17 and 18: Chemiluminescent Detection - ECL Pr
Page 19 and 20: Protein bound to membrane blocked w
Page 21 and 22: ECL Plex - relative quantitative We
Page 23 and 24: GE Healthcare Life Sciences Imaging
Page 25 and 26: CyDye TM Properties Very bright •
Page 27 and 28: The power of relating to house-keep
Page 29 and 30: Detection of proteins from rat brai
Page 31 and 32: Mr. Delaive Biochimie Cellulaire de
Page 33 and 34: Blue Native PAGE For the analysis o
Page 35 and 36: Nonionic detergents for solibilizat
Page 37 and 38: Steps after solubilization Incubati
Page 39 and 40: Buffer system for BN PAGE Blue load
Page 41 and 42: Blue Native- PAGE of Protein Comple
Page 43: 2D Blue Native/SDS-Electrophoresis
Page 47 and 48: 8 th Blue Native Electrophoresis Co
Page 49 and 50: Cationic Detergent PAGE For the ana
Page 51 and 52: Cationic detergent acidic gel PAGE
Page 53 and 54: DIGE: 16-BAC / SDS PAGE gels for hy
Page 55 and 56: Isoelectric Focusing Separation acc
Page 57 and 58: IEF with carrier ampholytes Pharmal
Page 59 and 60: Immobilized pH Gradients (IPG) Acry
Page 61 and 62: Two-dimensional Electrophoresis For
Page 63 and 64: Sample preparation Avoiding protein
Page 65 and 66: SDS in 2-D sample preparation Best
Page 67 and 68: Protein precipitation Clean-up from
Page 69 and 70: -Rat tongue tissue (26ug) -pH 3-10,
Page 71 and 72: Resolubilization possibilities •P
Page 73 and 74: The Trap platform Scale down from m
Page 75 and 76: Protein Enrichment products • NHS
Page 77 and 78: Make your own protein enrichment to
Page 79 and 80: Repeatability study: enrichment of
Page 81 and 82: Isoelectric Focusing Separation acc
Page 83 and 84: Principle of 2-D electrophoresis 1.
Page 85 and 86: Sample loading on pre-rehydrated IP
Page 87 and 88: IPGphor run recording graphs
Page 89 and 90: Streaking in basic pH region DTT an
Page 91 and 92: Micropreparative focusing with DeSt
Page 93 and 94: Basic gels: pH 7-11 NL New propriet
Page 95 and 96:
SDS PAGE chambers for Proteomics 25
Page 97 and 98:
Casting SDS gels and overlay gel ed
Page 99 and 100:
Wide pH gradient: 3 - 11 NL Mouse l
Page 102 and 103:
Protein Detection Methods Coomassie
Page 104 and 105:
Deep Purple Staining - fluorescent
Page 106 and 107:
Deep Purple - applications 2D gel 2
Page 108 and 109:
Carbamylation trains Impurities in
Page 110 and 111:
Salt in the gel Cell washing with P
Page 112 and 113:
Equilibration with SDS buffer 15 mi
Page 114 and 115:
2D Electrophoresis GE Healthcare Li
Page 116:
Amersham Biosciences AB, a General
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