- Page 1 and 2: - temperature + + + + + + + + + + +
- Page 3 and 4: SDS Polyacrylamide Gel Electrophore
- Page 5 and 6: Practical hints Always use Tris bas
- Page 7: Disc electrophoresis in readymade g
- Page 11 and 12: SDS electrophoresis SDS is an anion
- Page 13 and 14: Blotting and Specific Detection
- Page 15 and 16: 100ug Gel staining versus blotting
- Page 17 and 18: Chemiluminescent Detection - ECL Pr
- Page 19 and 20: Protein bound to membrane blocked w
- Page 21 and 22: ECL Plex - relative quantitative We
- Page 23 and 24: GE Healthcare Life Sciences Imaging
- Page 25 and 26: CyDye TM Properties Very bright •
- Page 27 and 28: The power of relating to house-keep
- Page 29 and 30: Detection of proteins from rat brai
- Page 31 and 32: Mr. Delaive Biochimie Cellulaire de
- Page 33 and 34: Blue Native PAGE For the analysis o
- Page 35 and 36: Nonionic detergents for solibilizat
- Page 37 and 38: Steps after solubilization Incubati
- Page 39 and 40: Buffer system for BN PAGE Blue load
- Page 41 and 42: Blue Native- PAGE of Protein Comple
- Page 43 and 44: 2D Blue Native/SDS-Electrophoresis
- Page 45 and 46: Resolution of mitochondrial superco
- Page 47 and 48: 8 th Blue Native Electrophoresis Co
- Page 49 and 50: Cationic Detergent PAGE For the ana
- Page 51 and 52: Cationic detergent acidic gel PAGE
- Page 53 and 54: DIGE: 16-BAC / SDS PAGE gels for hy
- Page 55 and 56: Isoelectric Focusing Separation acc
- Page 57 and 58: IEF with carrier ampholytes Pharmal
- Page 59 and 60:
Immobilized pH Gradients (IPG) Acry
- Page 61 and 62:
Two-dimensional Electrophoresis For
- Page 63 and 64:
Sample preparation Avoiding protein
- Page 65 and 66:
SDS in 2-D sample preparation Best
- Page 67 and 68:
Protein precipitation Clean-up from
- Page 69 and 70:
-Rat tongue tissue (26ug) -pH 3-10,
- Page 71 and 72:
Resolubilization possibilities •P
- Page 73 and 74:
The Trap platform Scale down from m
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Protein Enrichment products • NHS
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Make your own protein enrichment to
- Page 79 and 80:
Repeatability study: enrichment of
- Page 81 and 82:
Isoelectric Focusing Separation acc
- Page 83 and 84:
Principle of 2-D electrophoresis 1.
- Page 85 and 86:
Sample loading on pre-rehydrated IP
- Page 87 and 88:
IPGphor run recording graphs
- Page 89 and 90:
Streaking in basic pH region DTT an
- Page 91 and 92:
Micropreparative focusing with DeSt
- Page 93 and 94:
Basic gels: pH 7-11 NL New propriet
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SDS PAGE chambers for Proteomics 25
- Page 97 and 98:
Casting SDS gels and overlay gel ed
- Page 99 and 100:
Wide pH gradient: 3 - 11 NL Mouse l
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Protein Detection Methods Coomassie
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Deep Purple Staining - fluorescent
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Deep Purple - applications 2D gel 2
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Carbamylation trains Impurities in
- Page 110 and 111:
Salt in the gel Cell washing with P
- Page 112 and 113:
Equilibration with SDS buffer 15 mi
- Page 114 and 115:
2D Electrophoresis GE Healthcare Li
- Page 116:
Amersham Biosciences AB, a General