Downloaded by Louisiana State University on 19 September 2011 Published on 19 September 2011 on http://pubs.rsc.org | doi:10.1039/C1CC14578A Fig. 5 Kinetic plot for recombinant human NQO1 (1 10 5 g) towards 1 in pH 7.4, 0.1 M PBS, and 0.007% bovine serum albumin. Solid blue line indicates best fit to Michaelis<strong>–</strong>Menten equation. fluorophore 1 under physiological solution conditions is sensitive to the presence <strong>of</strong> hNQO1, while the de-cloaking is selective with regard to the presence <strong>of</strong> potential interferents. Importantly, 1 was designed to utilize ‘‘single-hit’’ enzyme activation; in other words, only a single stimulus is required to cause signal transduction (the release <strong>of</strong> 2). In addition, the signal revealing process for the activated species is efficient, as it requires only one step (hydroquinone lactonization). 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