PhD Thesis Demeter Zoltan

4.5.3 Amplifications................................................................................................42
4.5.3.1 RT-PCR assays .......................................................................................42
4.5.3.2 Classical PCR assays...............................................................................43
4.5.4 RFLP-based techniques..................................................................................43
4.5.4.1 Differentiation of vaccine and wild-type strains of CDV .......................43
4.5.4.2 Identification of type 2c CPVs................................................................43
4.5.5 Nucleic acid sequencing and phylogenetic analysis ......................................44
5. Results.........................................................................................................................45
5.1 Macroscopic findings............................................................................................45
5.1.1 CDV infections ..............................................................................................45
5.1.2 FPV infections................................................................................................47
5.1.3 CPV2 infection...............................................................................................48
5.2 Histopathology......................................................................................................48
5.2.1 CDV infection ................................................................................................49
5.2.2 FPV infection .................................................................................................49
5.2.3 CPV2 infection...............................................................................................49
5.3 Electron microscopy..............................................................................................51
5.4 Genetic investigations ...........................................................................................51
5.4.1 Amplifications................................................................................................51
5.4.2 RFLP-based investigations ............................................................................52
5.4.2.1 Differentiation of wild-type CDVs from vaccine strains........................52
5.4.2.2 Identification of type 2c CPVs................................................................54
5.4.3 Sequence analysis and phylogeny..................................................................55
5.4.3.1 CDV strains.............................................................................................55
5.4.3.2 FPV strains..............................................................................................65
5.4.3.3. CPV2 strains ..........................................................................................66
6. Discussion ...................................................................................................................68
7. New Scientific Results ................................................................................................79
8. References...................................................................................................................80
9. Scientific Publications of the Thesis...........................................................................94
10. Other Publications in Peer Reviewed Journals .........................................................95
11. Congress Abstracts....................................................................................................96
12. Acknowledgements...................................................................................................98
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