PhD Thesis Demeter Zoltan

many of them were type 2c CPVs. Based on the subsequent nucleic acid sequencing, all
Hungarian CPV2 strains turned out to be type 2a CPVs. The explanation of the initial
misleading results was a point mutation that occurred at the other end of the amplicons
used for the enzymatic digestion, emphasizing the need to constantly improve fast
genotyping techniques and the fact that initial results can be misleading and they have to
be double-checked using a more reliable technique.
The work also presents the first direct demonstration of a feline parvovirus (FPV)
infection in an Asia palm civet (Paradoxurus hermaphroditus). The pathological changes
induced by the infection were similar to those described in other species, but the result
emphasizes the fact that the host range of FPV is even wider than it was thought, and it
includes even members of the Viverridae family. FPV infection was also demonstrated in
a lion (Panthera leo) that belonged to a Hungarian lion tamer. The subsequent genetic
analysis of the Hungarian FPV strains from the Asian palm civet, lion and two cats
(Felis catus) revealed that there is relatively heterogenic group of FPV strains currently
circulating in Hungary.
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