Micropropagation and medicinal properties of Barleria greenii

Figure 3.6: Effects of pulsing with different IBA concentrations on ex vitro
acclimatization of regenerated shoots .............................................. 66
Chapter 4
Figure 4.1: In vitro propagation of Huernia hystrix. (A) Stock plant. (B) Control
with root production (MS medium without plant growth regulators).
(C) Multiple shoot production accompanied with root formation on MS
medium supplemented with 5.37 µM NAA and 22.19 µM BA. (D)
Two-month-old fully acclimatized plants (E) Green callus growth with
root hairs on MS medium supplemented with 5.37 µM NAA. (F) Root
regeneration from callus on MS medium supplemented with 2.69 µM
NAA and 2.22 µM BA. Scale bar = 10 mm. ...................................... 73
Figure 4.2: Frequencies of explants decontamination in different mercuric
chloride solution treatments. ............................................................ 74
Figure 4.3: Nocturnal titratable acidity in Huernia hystrix shoots cultured at
different temperatures. 22:00, 02:00 and 06:00 h are the beginning,
middle and end of 8 h dark period respectively. Bars with different
letters are significantly different (Ρ = 0.05) according to DMRT. ...... 84
Figure 4.4: Huernia hystrix adventitious shoot production from explants cultured
in different culture vessels. (A) Screw cap jar [300 ml]. (B) Culture
tube [40 ml]. Scale bar = 10 mm. ..................................................... 86
Figure 4.5: Effects of combinations of NAA and BA concentrations on callus
growth. Bars with different letters are significantly different (Ρ = 0.05)
according to DMRT. ........................................................................... 87
Chapter 5
Figure 5.1: Anti-inflammatory activity of extracts from different parts of three
Barleria species in COX-1 (on the left side) and COX-2 (on the right
side) assays. B.p, B.a, and B.g are Barleria prionitis, B. albostellata
and B. greenii, respectively. (A) and (B) are PE extracts. (C) and (D)
are DCM extracts. (E) and (F) are EtOH extracts. Bars in the same
graph bearing different letters are significantly different (P = 0.05)
according to DMRT. Percentage inhibition by indomethacin in COX-1
was 63.4 ± 1.98 and COX-2 was 73.6 ± 1.47. ................................ 111
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